Comparative metabolomics and transcriptomics of plant response to Tomato yellow leaf curl virus infection in resistant and susceptible tomato cultivars

Sade, Dagan; Shriki, Oz; Cuadros-Inostroza, Álvaro; Tohge, Takayuki; Semel, Yaniv; Haviv, Yaron; Willmitzer, Lothar; Fernie, Alisdair R.; Czosnek, H.; Brotman, Yariv

doi:10.1007/s11306-014-0670-x

Cited by 87 publications

(50 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When faced with pathogen infection, a defense system that combines physical and chemical barriers to protect the plant from damage is induced (Sade et al, 2015). Plant diseases can cause considerable losses to agriculture worldwide (Anderson et al, 2004).…”

Section: Genes In Response To Tomato Yellow Leaf Curly Virus Infectionmentioning

confidence: 99%

AP2/ERF Transcription Factors Involved in Response to Tomato Yellow Leaf Curly Virus in Tomato

et al. 2016

View full text Add to dashboard Cite

Tomato yellow leaf curly virus (TYLCV), transmitted by the whitefly (Bemisia tabaci), causes leaf curling and yellowing, plant dwarfism, and growth inhibition in tomato (Solanum lycopersicum L.). The APETALA2 (AP2) and ethylene response factor (ERF) transcription factor (TF) family, the largest plant-specific TF family, was identified to function in plant development and pathogen defense. Our study aimed to analyze the mechanism underlying the function of S. lycopersicum ERF (SlERF) TFs in response to TYLCV infection and improve useful information to increase the resistance to TYLCV in tomato. A total of 22 tomato AP2/ERF TFs in response to TYLCV were identified according to transcriptome database. Five ERF-B3 TFs were identified in cultivars Hongbeibei (highly resistant), Zheza-301, Zhefen-702 (both resistant), Jinpeng-1, and Xianke-6 (both susceptible). Interaction network indicated that SlERF TFs could interact with mitogen-activated protein kinase (MAPK). Expression profiles of five ERF-B3 genes (Soly19, Soly36, Soly66, Soly67, and Soly106) were detected by quantitative real-time-polymerase chain reaction (qRT-PCR) after TYLCV infection in five tomato cultivars. Soly106 expression was upregulated in five tomato cultivars. The expressions of three genes (Soly19, Soly67, and Soly36) were upregulated in Zheza-301 and Zhefen-702. Soly66 and Soly36 expressions were downregulated in Hongbeibei and Xianke-6, respectively. Yeast one-hybrid showed that the GCC-box binding ability of ERF-B3 TFs differed in resistant and susceptible tomato cultivars. Expression profiles were related to the GCC-box binding ability of SlERF TFs in resistant and susceptible tomato cultivars. The defense mechanism underlying the tomato's response to TYLCV involved a complicated network, which provided important information for us in breeding and genetic analysis.

show abstract

Section: Genes In Response To Tomato Yellow Leaf Curly Virus Infectionmentioning

confidence: 99%

AP2/ERF Transcription Factors Involved in Response to Tomato Yellow Leaf Curly Virus in Tomato

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Besides, more metabolomic studies on solanaceous species of crops have been carried out under different abiotic and biotic stresses. Comparative metabolomics to assess susceptibility of tomato to biotic stresses, such as tomato yellow leaf curl virus infection and root‐knot nematode infestation, revealed change in metabolite levels. In addition, most studies have reported effects of drought stress on concentration of different metabolites, for example in tobacco, tomato, potato and eggplant .…”

Section: Introductionmentioning

confidence: 99%

Metabolomic analyses to evaluate the effect of drought stress on selected African Eggplant accessions

et al. 2017

View full text Add to dashboard Cite

BACKGROUND: Drought stress is one of the main abiotic stresses that affect crops. It leads to biochemical changes that can have adverse effects on plant growth, development and productivity. African eggplants are important vegetable and fruit crops reported to adapt and thrive well under drought stress. The diversified metabolites arising due to stress have not been well defined. A gas chromatographic-mass spectrometric metabolomic approach was applied to characterize the effect of drought stress on metabolites at different stages of growth. Nineteen accessions were selected for analysis and drought was imposed by withholding water until soil moisture reached 60% field capacity. Fresh leaf tissues were sampled before stress, 2 and 4 weeks after stress and metabolite profiling done.

show abstract

“…Given their key importance, these metabolite responses could also be used as "biomarkers" to indicate the health or disease status in plants and provide further insight into the pathogenicity of the disease agent. Using gas chromatography-coupled mass spectrometry (GC-MS), metabolite biomarkers have been identified for plant diseases such as Tomato yellow leaf curl virus (Sade et al, 2014), Ganoderma disease in oil palm (Nusaibah, Siti Nor Akmar, Idris, Sariah, & Mohamad, 2016), and Fusarium infection in maize (Sherif et al, 2016). For Armillaria disease, pine seedlings infected with Armillaria ostoyae exhibited distinct levels of certain metabolites 9-month post-inoculation despite there being no visible symptoms (Isidorov, Lech, Żółciak, Rusak, & Szczepaniak, 2008).…”

mentioning

confidence: 99%

Comparative metabolomics implicates threitol as a fungal signal supporting colonization of Armillaria luteobubalina on eucalypt roots

Wong-Bajracharya

Plett

Natera

et al. 2019

Plant Cell & Environment

View full text Add to dashboard Cite

Armillaria root rot is a fungal disease that affects a wide range of trees and crops around the world. Despite being a widespread disease, little is known about the plant molecular responses towards the pathogenic fungi at the early phase of their interaction. With recent research highlighting the vital roles of metabolites in plant root–microbe interactions, we sought to explore the presymbiotic metabolite responses of Eucalyptus grandis seedlings towards Armillaria luteobuablina, a necrotrophic pathogen native to Australia. Using a metabolite profiling approach, we have identified threitol as one of the key metabolite responses in E. grandis root tips specific to A. luteobubalina that were not induced by three other species of soil‐borne microbes of different lifestyle strategies (a mutualist, a commensalist, and a hemi‐biotrophic pathogen). Using isotope labelling, threitol detected in the Armillaria‐treated root tips was found to be largely derived from the fungal pathogen. Exogenous application of d‐threitol promoted microbial colonization of E. grandis and triggered hormonal responses in root cells. Together, our results support a role of threitol as an important metabolite signal during eucalypt‐Armillaria interaction prior to infection thus advancing our mechanistic understanding on the earliest stage of Armillaria disease development. Comparative metabolomics of eucalypt roots interacting with a range of fungal lifestyles identified threitol enrichment as a specific characteristic of Armillaria pathogenesis. Our findings suggest that threitol acts as one of the earliest fungal signals promoting Armillaria colonization of roots.

show abstract

Comparative metabolomics and transcriptomics of plant response to Tomato yellow leaf curl virus infection in resistant and susceptible tomato cultivars

Cited by 87 publications

References 86 publications

AP2/ERF Transcription Factors Involved in Response to Tomato Yellow Leaf Curly Virus in Tomato

AP2/ERF Transcription Factors Involved in Response to Tomato Yellow Leaf Curly Virus in Tomato

Metabolomic analyses to evaluate the effect of drought stress on selected African Eggplant accessions

Comparative metabolomics implicates threitol as a fungal signal supporting colonization of Armillaria luteobubalina on eucalypt roots

Contact Info

Product

Resources

About