Comparative morphological evaluation of domestic animal cornea

Nautscher, Nadine; Bauer, Achim; Šteffl, Martin; Amselgruber, Werner

doi:10.1111/vop.12298

Cited by 43 publications

(87 citation statements)

References 25 publications

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“…The anterior epitheliumstratum superficiale consisted of four to five rows of cells. This is in accordance with the findings of Nautscher et al (2016), who found that the corneal stroma in domestic animals was also the thickest layers and consisted of a uniform collagen fibril matrix. In carnivores, this epithelium consisted of six to nine cell rows; in horses 12-15 cell rows; in cattle 11-15 cells rows; in pigs and sheep 8-12 rows of squamous cells; and in the dromedary camel two to three cell rows.…”

Section: Discussionsupporting

confidence: 93%

“…The large differences in the number of cell layers were observed in the stratum superficiale in the corneal epithelium (Meridano et al, 1998;Nautscher et al, 2016). In our study, the anterior basement membrane was weakly PAS positive in the Sulawesi bear cuscus and consisted of thin collagen fibres, while in domestic animals (Nautscher et al, 2016) the anterior basement membrane was PAS positive. The anterior epitheliumstratum superficiale consisted of four to five rows of cells.…”

Section: Discussionsupporting

confidence: 41%

“…Studies carried out in domestic animals revealed various numbers of rows of cells (Nautscher et al, 2016;Saadatlou, 2017). The study by Nautscher et al (2016) and Saadatlou (2017) reported a varying thickness of Descemet's membrane that ranged from 18.2 (±2.7 μm) in pigs to 109.1 (±8.5 μm) in horses and 15-20 μm in dromedary camels. In our study, the anterior basement membrane was weakly PAS positive in the Sulawesi bear cuscus and consisted of thin collagen fibres, while in domestic animals (Nautscher et al, 2016) the anterior basement membrane was PAS positive.…”

Section: Discussionmentioning

confidence: 98%

“…In mammals, it consists of the anterior non-keratinising stratified squamous epithelium underlain by a Bowman's layers, the corneal stroma, Descemet's membrane and posterior corneal endothelium (König & Liebich, 2006;Maurice, 1957;Nautscher, Bauer, Steffl, & Amselgruber, 2016;Nickel et al, 2004;Pedler, 1962). In mammals, it consists of the anterior non-keratinising stratified squamous epithelium underlain by a Bowman's layers, the corneal stroma, Descemet's membrane and posterior corneal endothelium (König & Liebich, 2006;Maurice, 1957;Nautscher, Bauer, Steffl, & Amselgruber, 2016;Nickel et al, 2004;Pedler, 1962).…”

Section: Discussionmentioning

confidence: 99%

“…In mammals, it consists of the anterior non-keratinising stratified squamous epithelium underlain by a Bowman's layers, the corneal stroma, Descemet's membrane and posterior corneal endothelium (König & Liebich, 2006;Maurice, 1957;Nautscher, Bauer, Steffl, & Amselgruber, 2016;Nickel et al, 2004;Pedler, 1962). Interestingly, there are morphological differences in the size and number of cells in the corneal layers among various animal species and with regard to the common human corneal structure (Hayashi et al, 2002;Nautscher et al, 2016). Interestingly, there are morphological differences in the size and number of cells in the corneal layers among various animal species and with regard to the common human corneal structure (Hayashi et al, 2002;Nautscher et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Microstructure of the eye tunics, eyelids and ocular glands of the Sulawesi bear cuscus (Ailurops ursinusTemminck, 1824) (Phalangeridae: Marsupialia) based on anatomical, histological and histochemical studies

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Darska

et al. 2018

Acta Zoologica

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This study described the anatomy, histology and the histochemical analysis of the eye tunics, the upper and lower eyelid, the third eyelid, the lacrimal gland and the superficial gland of the third eyelid in adult Sulawesi bear cuscus. The eyeball and the eyelids with the orbital glands were harvested immediately post‐mortem. The eyeball in the Sulawesi bear cuscus had a sphere‐like shape. The pupil was round, and the lens was a circular biconvex body. There was neither tapetum lucidum nor Harderian gland. Similarly, there were no eyelashes in the lower eyelid. The lymphoid follicles and the high endothelial venules (HEV) were found in the lymphoid region only in the third eyelid and in the connective tissue of the superficial gland of the third eyelid. The third eyelid in the bear cuscus resembled the letter “T.” The lacrimal gland and superficial gland of the third eyelid were multilobar tubuloacinar glands. The histological analysis and histochemical studies showed that the lacrimal gland in the Sulawesi bear cuscus produced a mucoserous secretion with predominantly serous cells. In contrast, the superficial gland of the third eyelid produced a serous secretion with a single acinus mucous in character.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 41%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

“…In mammals, it consists of the anterior non-keratinising stratified squamous epithelium underlain by a Bowman's layers, the corneal stroma, Descemet's membrane and posterior corneal endothelium (König & Liebich, 2006;Maurice, 1957;Nautscher, Bauer, Steffl, & Amselgruber, 2016;Nickel et al, 2004;Pedler, 1962). Interestingly, there are morphological differences in the size and number of cells in the corneal layers among various animal species and with regard to the common human corneal structure (Hayashi et al, 2002;Nautscher et al, 2016). Interestingly, there are morphological differences in the size and number of cells in the corneal layers among various animal species and with regard to the common human corneal structure (Hayashi et al, 2002;Nautscher et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Microstructure of the eye tunics, eyelids and ocular glands of the Sulawesi bear cuscus (Ailurops ursinusTemminck, 1824) (Phalangeridae: Marsupialia) based on anatomical, histological and histochemical studies

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Darska

et al. 2018

Acta Zoologica

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A new perspective on the corneo‐scleral junction with three types of microscopy techniques

Kose

Orhan

Alan

et al. 2023

Microscopy Res & Technique

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The conjunctions of the cornea and sclera in the eyes of donkeys, cattle, dogs, sheep, pigs and rabbits, regardless of gender, were examined in this study. No animals were specifically sacrificed for this investigation. Scanning electron microscopy, light microscopy, and dissecting microscopy were used in this research. In the limbus of all the animals investigated, the cornea and sclera fused in accordance with a pattern. At the corneo‐scleral junction, the sclera was situated anteriorly and the cornea posteriorly in the dorsal and ventral sections of the bulbus oculi. In the medial and lateral parts of the eyeball, the cornea and sclera were facing each other and interlaced. Pigmentation and the sulcus scleralis externus could be used to identify the macro‐and micro‐anatomical boundaries of the limbus. In addition, the cytoplasm of basal epithelial cells shrank, signaling the end of the corneal epithelium and the start of the conjunctival epithelium. The presence of Bowman's membrane in cattle and sheep eyes was definitely determined in histological examinations. Bowman's membrane in these animals came to an end at the limbus, which is where the conjunctival epithelium starts and the corneal epithelium ends. In all areas of the cornea, Bowman's membrane revealed irregular, abrupt thickening and thinning. The corneal epithelium was thick in the vertex and thinner towards the limbus, whereas Descemet's membrane was thin in the center (vertex) and thick in the periphery (near the limbus). In this study, pictures and diagrams were used to illustrate the general anatomical, histological, and morphometric characteristics of the limbus in the species under investigation. The data from our study showed that the limbus region of the bulbus oculi was narrow in the lateral and medial parts and wide in the dorsal and ventral parts. This was confirmed in the studied animals as a general rule. The width value will undoubtedly affect the number of cells covered by the regions. It is conceivable that these cells will play a significant role in the decision of where to perform surgical procedures in order to promote wound healing, giving doctors an advantage. In this circumstances, we think that the limbus should be studied in terms of clinical methods because it has different shapes depending on the species and the position of the bulbus.

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An eye on the dog as the scientist's best friend for translational research in ophthalmology: Focus on the ocular surface

Sebbag

Mochel

2020

Medicinal Research Reviews

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Preclinical animal studies provide valuable opportunities to better understand human diseases and contribute to major advances in medicine. This review provides a comprehensive overview of ocular parameters in humans and selected animals, with a focus on the ocular surface, detailing species differences in ocular surface anatomy, physiology, tear film dynamics and tear film composition. We describe major pitfalls that tremendously limit the translational potential of traditional laboratory animals (i.e., rabbits, mice, and rats) in ophthalmic research, and highlight the benefits of integrating companion dogs with clinical analogues to human diseases into preclinical pharmacology studies. This One Health approach can help accelerate and improve the framework in which ophthalmic research is translated to the human clinic. Studies can be conducted in canine subjects with naturally occurring or noninvasively induced ocular surface disorders (e.g., dry eye disease, conjunctivitis), reviewed herein, and tear fluid can be easily retrieved from canine eyes for various bioanalytical purposes. In this review, we discuss common tear collection methods, including capillary tubes and Schirmer tear strips, and provide guidelines for tear sampling and extraction to improve the reliability of analyte quantification (drugs, proteins, others).

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Comparative morphological evaluation of domestic animal cornea

Abstract: Differences in the distribution of CKs and AQPs in various species suggest species differences in the maintenance of structural integrity and fluid balance.

Cited by 43 publications

References 25 publications

Microstructure of the eye tunics, eyelids and ocular glands of the Sulawesi bear cuscus (Ailurops ursinusTemminck, 1824) (Phalangeridae: Marsupialia) based on anatomical, histological and histochemical studies

Microstructure of the eye tunics, eyelids and ocular glands of the Sulawesi bear cuscus (Ailurops ursinusTemminck, 1824) (Phalangeridae: Marsupialia) based on anatomical, histological and histochemical studies

A new perspective on the corneo‐scleral junction with three types of microscopy techniques

An eye on the dog as the scientist's best friend for translational research in ophthalmology: Focus on the ocular surface

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