Comparative physiology and transcriptome analysis reveals the regulatory mechanism of genome duplication enhancing cold resistance in Fragaria nilgerrensis

Liu, Jie; Li, Junmin; Fu, Chengxin

doi:10.1016/j.envexpbot.2021.104509

Cited by 9 publications

(14 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the regulation of a number of other key genes involved in CIS is unclear [ 29 ]. Polyploid plants are often associated with better tolerance to abiotic stresses over their diploid progenitors [ 3 ], such as cold [ 30 ]. Thus, understanding the expression and epigenetic regulation of CIS-related genes at different ploidy levels may provide clues for improving resistance to CIS in potato.…”

Section: Introductionmentioning

confidence: 99%

Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers

Guo

Zhou

Zhang

et al. 2023

Horticulture Research

View full text Add to dashboard Cite

Summary Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts. Extensive studies have shown genetic, transcriptomic and epigenetic dynamics upon polyploidization in multiple plant species. However, few studies are dedicated to unveil those alternations imposed only by ploidy level, without any interference from heterozygosity. Cultivated potato is highly heterozygous. Thus, in this study, we developed two homozygous autotetraploid lines and one homozygous diploid line in parallel from a homozygous diploid potato. We confirmed their ploidy levels using chloroplast counting and karyotyping. Oligo-FISH and genome re-sequencing validated that these potato lines are nearly homozygous. We investigated variations in phenotypes, transcription and histone modifications between two ploidies. Both autotetraploid lines produced larger but fewer tubers than the diploid line. Interestingly, each autotetraploid line displayed ploidy-related differential expression for various genes. We also discovered a genome-wide enrichment of H3K27ac in genic regions upon whole-genome doubling (WGD). However, such enrichment was not associated with the differential gene expression between two ploidies. The tetraploid lines may exhibit better resistance to cold-induced sweetening (CIS) than the diploid line in tubers, potentially regulated through the expression of CIS-related key genes, which seems to be associated with the levels of H3K4me3 in cold-stored tubers. These findings will help to understand the impacts of autotetraploidization on dynamics of phenotypes, transcription and histone modifications, as well as on CIS-related genes in response to cold storage.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers

Guo

Zhou

Zhang

et al. 2023

Horticulture Research

View full text Add to dashboard Cite

show abstract

“…Ca 2+ is a key signal in the cold response network of plant cells ( Guo et al., 2018 ). The tetraploid cold-tolerant strawberry has a higher expression level of the calcium channel protein so as to ensure the normal transmission of cold stress signals with the fluctuation of calcium ion concentration ( Liu et al., 2021a ). Calcium-binding protein senses the temperature variation through the change of intracellular calcium level, decodes it as a stress signal, and transmits it downstream as a master switch to control various stress genes ( Mahajan and Tuteja, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

“…Calcium-binding protein senses the temperature variation through the change of intracellular calcium level, decodes it as a stress signal, and transmits it downstream as a master switch to control various stress genes ( Mahajan and Tuteja, 2005 ). Therefore, P. crymophila may rely on calcium-related proteins to utilize calcium ions as cold stress signals to regulate the expression of downstream stress-responsive genes such as CBFs and CORs and finally produce physiological and biochemical responses of cold stress defense such as cell wall reinforcement, hypersensitive response, and stomatal closure ( Liu et al., 2021a ).…”

Section: Discussionmentioning

confidence: 99%

“…Weighted gene co-expression network analysis (WGCNA) is an efficient and powerful method for constructing a co-expression network and digging into core genes. In this way, four pivotal genes of CAD , POD , CCoAMT , and CML were found in strawberries ( Fragaria nilgerrensis ) under cold stress, which has the key function of controlling high cold resistance ( Liu et al., 2021a ). Similarly, by comparing with the transcriptome of cold-tolerant and cold-sensitive varieties of Chinese prickly ash ( Zanthoxylum bungeanum Maxim), the top 150 hub genes were identified and the mechanism of cold stress was discussed ( Tian et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Flexible response and rapid recovery strategies of the plateau forage Poa crymophila to cold and drought

Wang²,

Hou³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Cold and drought stress are the two most severe abiotic stresses in alpine regions. Poa crymophila is widely grown in the Qinghai–Tibet Plateau with strong tolerance. Here, by profiling gene expression patterns and metabolomics-associated transcriptomics co-expression network, the acclimation of Poa crymophila to the two stresses was characterized. (1) The genes and metabolites with stress tolerance were induced by cold and drought, while those related with growth were inhibited, and most of them were restored faster after stresses disappeared. In particular, the genes for the photosynthesis system had strong resilience. (2) Additionally, cold and drought activated hypoxia and UV-B adaptation genes, indicating long-term life on the plateau could produce special adaptations. (3) Phenolamines, polyamines, and amino acids, especially N′,N″,N′″-p-coumaroyl-cinnamoyl-caffeoyl spermidine, putrescine, and arginine, play key roles in harsh environments. Flexible response and quick recovery are strategies for adaptation to drought and cold in P. crymophila, accounting for its robust tolerance and resilience. In this study, we presented a comprehensive stress response profile of P. crymophila and provided many candidate genes or metabolites for future forage improvement.

show abstract

“…Studies have shown that the CCoAOMT gene and the CAD gene are not only involved in lignin and flavonoid synthesis in the phenylpropanoid biosynthesis pathway but also related to plant resistance [ 10 , 11 ]. When plants are under the conditions of drought [ 12 ], low temperature [ 12 , 13 ], salt stress [ 14 , 15 ], hormone induction [ 15 ] and pathogen infection [ 16 , 17 ], CCoAOMT and CAD participate in the mechanisms of plant stress responses. However, research on the CCoAOMT gene and the CAD gene in bamboo generally focuses on gene cloning and expression analysis.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Phenylpropane-Synthesis-Related Genes Regulating the Shoot Blight Resistance of Bambusa pervariabilis × Dendrocalamopsis grandis

Luo

Peng

Xie

et al. 2022

IJMS

View full text Add to dashboard Cite

Bambusa pervariabilis × Dendrocalamopsis grandis shoot blight caused by Arthrinium phaeospermum is a fungal disease that has affected a large area in China in recent years. However, it is not clear which genes are responsible for the disease resistance of B. pervariabilis × D. grandis. Based on the analysis of transcriptome and proteome data, two genes, CCoAOMT2 and CAD5, which may be involved in disease resistance, were screened. Two gene expression-interfering varieties, COF RNAi and CAD RNAi were successfully obtained using RNAi technology. Quantitative real-time fluorescence (qRT-PCR) results showed that CCoAOMT2 gene, CAD5 gene and seven related genes expression was down-regulated in the transformed varieties. After inoculating pathogen spore suspension, the incidence and disease index of cof-RNAi and cad-RNAi transformed plants increased significantly. At the same time, it was found that the content of total lignin and flavonoids in the two transformed varieties were significantly lower than that of the wild-type. The subcellular localization results showed that both CCoAOMT2 and CAD5 were localized in the nucleus and cytoplasm. The above results confirm that the CCoAOMT2 and CAD5 genes are involved in the resistance of B. pervariabilis × D.grandis to shoot blight through regulating the synthesis of lignin and flavonoids.

show abstract

Comparative physiology and transcriptome analysis reveals the regulatory mechanism of genome duplication enhancing cold resistance in Fragaria nilgerrensis

Cited by 9 publications

References 95 publications

Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers

Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers

Flexible response and rapid recovery strategies of the plateau forage Poa crymophila to cold and drought

Molecular Mechanisms of Phenylpropane-Synthesis-Related Genes Regulating the Shoot Blight Resistance of Bambusa pervariabilis × Dendrocalamopsis grandis

Contact Info

Product

Resources

About