Comparative profiling of human saliva by intact protein LC/ESI-TOF mass spectrometry

Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study

“…This was done in light of future potential clinical applications in which an objective measurement of acute stress may be performed directly in the workplace or in a clinic to provide rapid results and to attempt to mitigate the stressor or any negative consequences of performing delicate tasks (e.g., medical and military tasks) when possible. It was noted previously that it is more practical to use equal volumes of saliva for analysis as many clinical environments do not routinely perform protein assays before analysis (Millea et al ., 2007). …”

“…Saliva can be analyzed for biomarkers as it reflects many local and systemic physiological and biochemical processes, and its secretion is regulated by the autonomic nervous system (Amado et al ., 2005, Al-Tarawneh et al ., 2011, Brandão et al ., 2014). Overall, saliva is a mixture of electrolytes, proteins, small organic compounds, and nucleic acids (Vitorino et al ., 2004, Millea et al ., 2007, Brandão et al ., 2014). The predominant salivary protein families include amylases, mucins, cystatins, histatins, acidic proline-rich proteins, and basic proline-rich proteins (Denny et al ., 2008, Scarano et al ., 2010, Castagnola et al ., 2011, Castagnola et al ., 2012, Amado et al ., 2013, Wu et al ., 2014).…”

The Denture-Associated Oral Microbiome in Health and Stomatitis

“…One important host factor involved in this phenomenon could be saliva, which coats available natural, as well as artificial, oral surfaces with a so-called pellicle (33). Saliva can display large variability between individuals (34, 35) and provides important adhesion proteins for bacterial attachment (36). In addition, our discovery that intrapatient factors could be stronger determinants of bacterial biofilm community composition than different surfaces emphasizes that grouping and pooling of samples from different people can influence analysis outcome.…”

Quantitative mass spectrometry as a tool for nutritional proteomics

“…This means that running technical replicates can verify results for abundant proteins but is unlikely to provide confirmation of results for less abundant proteins, as was observed in an analysis of human saliva. The abundance of the 20 most abundant proteins varied by only 10% between replicate runs, whereas less abundant proteins were sampled sporadically and had greater variation (45). It is interesting that the study by Millea et al also found significant variation between persons, which highlights the difficulty of interpreting highly specific and sensitive MS data, given the wide ranges observed in the general population (45,46).…”