Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC–MS/MS

Dai, Longjun; Kang, Guijuan; Nie, Zhiyi; Li, Yu; Zeng, Rizhong

doi:10.1016/j.jprot.2015.11.012

Cited by 31 publications

(23 citation statements)

References 34 publications

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“…Recent studies in rubber tree revealed that this protein causes laticifer plugging, as it may assist in the aggregation of rubber particles. Moreover, abundances of both the sieve element occlusion protein and its mRNA were reduced after etephon treatment, which facilitated and may have a direct relationship with latex flow [ 36 ]. Although different versions of the rubber tree genome have recently been published [ 23 – 25 ], a genome annotation is not publicly available.…”

Section: Discussionmentioning

confidence: 99%

QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions

et al. 2018

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BackgroundRubber tree is cultivated in mainly Southeast Asia and is by far the most significant source of natural rubber production worldwide. However, the genetic architecture underlying the primary agronomic traits of this crop has not been widely characterized. This study aimed to identify quantitative trait loci (QTLs) associated with growth and latex production using a biparental population established in suboptimal growth conditions in Brazil.ResultsA full-sib population composed of 251 individuals was developed from crossing two high-producing Asiatic rubber tree cultivars, PR 255 and PB 217. This mapping population was genotyped with microsatellite markers from enriched genomic libraries or transcriptome datasets and single-nucleotide polymorphism (SNP) markers, leading to construction of a saturated multipoint integrated genetic map containing 354 microsatellite and 151 SNP markers. Height and circumference measurements repeated over a six-year period and registration of cumulative latex production during six consecutive months on the same individuals allowed in-depth characterization of the genetic values of several growth traits and precocious latex production. Growth traits, circumference and height, were overall positively correlated, whereas latex production was not correlated or even negatively correlated with growth traits. A total of 86 distinct QTLs were identified, most of which were detected for only one trait. Among these QTLs, 15 were linked to more than one phenotypic trait (up to 4 traits simultaneously). Latex production and circumference increments during the last wintering period were associated with the highest numbers of identified QTLs (eleven and nine, respectively), jointly explaining the most significantly observed phenotypic variances (44.1% and 44.4%, respectively). The most important QTL for latex production, located on linkage group 16, had an additive effect of the male parent PB 217 and corresponded to a QTL at the same position detected in a previous study carried out in Thailand for the biparental population RRIM 600 x PB 217.ConclusionsOur results identified a set of significant QTLs for rubber tree, showing that the performance of modern Asiatic cultivars can still be improved and paving the way for further marker-assisted selection, which could accelerate breeding programs.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1450-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions

et al. 2018

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“…Recently, many proteins had been identified by proteomics-based technologies from different Hevea latex components, such as total latex ( D’Amato et al, 2010 ; Li et al, 2011 ; Wang et al, 2015 ; Dai et al, 2016 ; Habib et al, 2017 ), rubber particles ( Rojruthai et al, 2010 ; Xiang et al, 2012 ; Dai et al, 2013 , 2016 ; Wang et al, 2016 ; Yamashita et al, 2016 ), C-serum ( Wagner et al, 2007 ; Wang et al, 2010 ; Havanapan et al, 2016 ), and lutoids ( Wang et al, 2010 , 2013 ; Habib et al, 2017 ). Proteins involved in mevalonate (MVA) pathway are crucial for NRB ( Wang et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex

Gao

Sun

et al. 2018

Front. Plant Sci.

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Molybdenum (Mo) is an essential micronutrient in many plants. In the rubber tree Hevea brasiliensis, Mo application can reduce the shrinkage of the tapping line, decrease tapping panel dryness, and finally increase rubber latex yield. After combined Mo with ethylene (Eth), these effects become more obvious. However, the molecular mechanism remains unclear. Here, we compared the changed patterns of physiological parameters and protein accumulation in rubber latex after treated with Mo and/or Eth. Our results demonstrated that both Eth and Mo can improve the contents of thiol, sucrose, and dry yield in rubber latex. However, lutoid bursting is significantly inhibited by Mo. Comparative proteomics identified 169 differentially expressed proteins, including 114 unique proteins, which are mainly involved in posttranslational modification, carbohydrate metabolism, and energy production. The abundances of several proteins involved in rubber particle aggregation are decreased upon Mo stimulation, while many enzymes related to natural rubber biosynthesis are increased. Comparison of the accumulation patterns of 25 proteins revealed that a large portion of proteins have different changed patterns with their gene expression levels. Activity assays of six enzymes revealed that Mo stimulation can increase latex yield by improving the activity of some Mo-responsive enzymes. These results not only deepen our understanding of the rubber latex proteome but also provide new insights into the molecular mechanism of Mo-stimulated rubber latex yield.

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“…These differentially expressed seed proteins were involved in primary and secondary metabolism, nucleic acid and amino acid metabolism, and abscisic acid-related responses, corroborating the physiological effects of AGG3 overexpression in providing protection against oxidative and heavy metal stress conditions. Recent investigations of the proteome of the latex of the allotetraploid rubber tree (Hevea brasiliensis) [132,133] found that the proteomes of rubber trees susceptible to or tolerant of Phytophthora sp. had comparable protein counts [132].…”

Section: Additive and Non-additive Protein Expression In Allopolyploidsmentioning

confidence: 99%

Polyploidy and the proteome

Soltis

Misra

Shan

et al. 2016

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Although major advances have been made during the past 20 years in our understanding of the genetic and genomic consequences of polyploidy, our knowledge of polyploidy and the proteome is in its infancy. One of our goals is to stimulate additional study, particularly broad-scale proteomic analyses of polyploids and their progenitors. Although it may be too early to generalize regarding the extent to which transcriptomic data are predictive of the proteome of polyploids, it is clear that the proteome does not always reflect the transcriptome. Despite limited data, important observations on the proteomes of polyploids are emerging. In some cases, proteomic profiles show qualitatively and/or quantitatively non-additive patterns, and proteomic novelty has been observed. Allopolyploids generally combine the parental contributions, but there is evidence of parental dominance of one contributing genome in some allopolyploids. Autopolyploids are typically qualitatively identical to but quantitatively different from their parents. There is also evidence of parental legacy at the proteomic level. Proteomes clearly provide insights into the consequences of genomic merger and doubling beyond what is obtained from genomic and/or transcriptomic data. Translating proteomic changes in polyploids to differences in morphology and physiology remains the holy grail of polyploidy--this daunting task of linking genotype to proteome to phenotype should emerge as a focus of polyploidy research in the next decade. This article is part of a Special Issue entitled: Plant Proteomics--a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock.

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Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC–MS/MS

Cited by 31 publications

References 34 publications

QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions

QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions

Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex

Polyploidy and the proteome

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