Comparative proteomics of respiratory exosomes in cystic fibrosis, primary ciliary dyskinesia and asthma

Rollet-Cohen, Virginie; Bourderioux, Matthieu; Lipecka, Joanna; Chhuon, Cérina; Jung, Vincent; Mesbahi, Myriam; Nguyen–Khoa, Thao; Guérin-Pfyffer, Sophie; Schmitt, Alain; Edelman, Aleksander; Sermet‐Gaudelus, Isabelle; Guerrera, Ida Chiara

doi:10.1016/j.jprot.2018.07.001

Cited by 39 publications

(38 citation statements)

References 44 publications

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“…micropatricles/ectosomes (100-500 nm) 6 and exomeres (30-70 nm) 7 have also been reported to be biologically significant in disease states. A recent study identified proinflammatory signatures in respiratory vesicles from a small number of PWCF 8 highlighting the potential of EVs in modulating inflammation in CF. 9 We hypothesised that EVs released from CF lung epithelial cells play a role in regulating leukocyte migration into the CF airways and may help understand disease pathogenesis in CF.…”

Section: Why Read On?mentioning

confidence: 99%

Increased extracellular vesicles mediate inflammatory signalling in cystic fibrosis

Useckaite

Ward

Trappe

et al. 2020

Thorax

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RationaleMutations in the cystic fibrosis transmembrane regulator (CFTR) gene form the basis of cystic fibrosis (CF). There remains an important knowledge gap in CF as to how diminished CFTR activity leads to the dominant inflammatory response within CF airways.ObjectivesTo investigate if extracellular vesicles (EVs) contribute to inflammatory signalling in CF.MethodsEVs released from CFBE41o-, CuFi-5, 16HBE14o- and NuLi-1 cells were characterised by nanoparticle tracking analysis (NTA). EVs isolated from bronchoalveolar lavage fluid (BALF) from 30 people with CF (PWCF) were analysed by NTA and mass spectrometry and compared with controls. Neutrophils were isolated from the blood of 8 PWCF to examine neutrophil migration in the presence of CFBE41o- EVs.ResultsA significantly higher level of EVs were released from CFBE41o- (p<0.0001) and CuFi-5 (p=0.0209) relative to control cell lines. A significantly higher level of EVs were detected in BALF of PWCF, in three different age groups relative to controls (p=0.01, 0.001, 0.002). A significantly lower level of EVs were released from CFBE41o- (p<0.001) and CuFi-5 (p=0.0002) cell lines treated with CFTR modulators. Significant changes in the protein expression of 126 unique proteins was determined in EVs obtained from the BALF of PWCF of different age groups (p<0.001–0.05). A significant increase in chemotaxis of neutrophils derived from PWCF was observed in the presence of CFBE41o EVs (p=0.0024) compared with controls.ConclusionThis study demonstrates that EVs are produced in CF airway cells, have differential protein expression at different ages and drive neutrophil recruitment in CF.

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Section: Why Read On?mentioning

confidence: 99%

Increased extracellular vesicles mediate inflammatory signalling in cystic fibrosis

Useckaite

Ward

Trappe

et al. 2020

Thorax

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“…Rollet-Cohen et al compared the proteomic content of respiratory exosomes from cystic fibrosis, primary ciliary dyskinesia and asthma patients. The results showed high expression of LCN2 in exosomes from cystic fibrosis patients (44). The above studies suggest that EVs may contain bonederived factors and play a regulatory role in physiological or pathological conditions.…”

Section: Bone-secreted Factors Transferred By Evsmentioning

confidence: 65%

Bone-Derived Extracellular Vesicles: Novel Players of Interorgan Crosstalk

Yin

Guo

et al. 2019

Front. Endocrinol.

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An increasing number of studies have shown that bone plays an active role in regulating glucose metabolism, affects renal, and cardiovascular diseases and even influences the development of offspring. These novel findings have indicated that bone plays a much more important role in the human body than only providing physical support. However, further investigations of the mechanisms underlying the effects of bone are needed. Recently, extracellular vesicles (EVs) have received increased attention because they can transfer functional proteins, mRNAs, and miRNAs between cells/organs. After reviewing the existing evidence, we hypothesized that bone may be involved in interorgan communication via EVs. Further research exploring bone-derived EVs may facilitate the understanding of bone as a multifunctional organ.

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“…Exosomes from asthma patients contained lower amounts of grancalcin and histones, contained Serpin A6, a protein involved in counter‐regulation of immune proteinases, and APOA4, a protein playing a role in lipid and surfactant metabolism. Most proteins identified are known activators of the NF‐κB pathway and can contribute to the pro‐inflammatory status of these respiratory diseases …”

Section: Resultsmentioning

confidence: 99%

“…Lipids such as ceramides, ceramide-phosphates, phosphatidylglycerol and sphingomyelins, which are mediators of inflammation in asthma, were found to be increased in EVs derived from asthma patients 74. A study by Rollet-Cohen et al75 used proteomics to compare respiratory exosomal contents isolated from BALF of patients suffering from cystic fibrosis (CF), primary ciliary dyskinesia (PCD) and asthma. Exosomes from asthma patients contained lower amounts of grancalcin and histones, contained Serpin A6, a protein involved in counter-regulation of immune proteinases, and APOA4, a protein playing a role in lipid and surfactant metabolism.…”

mentioning

confidence: 99%

Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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Asthma is a chronic, recurrent and incurable allergy‐related respiratory disease characterized by inflammation, bronchial hyperresponsiveness and narrowing of the airways. Extracellular vesicles (EVs) are a universal feature of cellular function and can be detected in different bodily fluids. Recent evidence has shown the possibility of using EVs in understanding the pathogenesis of asthma, including their potential as diagnostic and therapeutic tools. Studies have reported that EVs released from key cells involved in asthma can induce priming and activation of other asthma‐associated cells. A literature review was conducted on all current research regarding the role and function of EVs in the pathogenesis of asthma via the PRISMA statement method. An electronic search was performed using EMBASE and PubMed through to November 2018. The EMBASE search returned 76 papers, while the PubMed search returned 211 papers. Following duplicate removal, titles and abstracts were screened for eligibility with a total of 34 studies included in the final qualitative analysis. The review found evidence of association between the presence of EVs and physiological changes characteristic of asthma, suggesting that EVs are involved in the pathogenesis, with the weight of evidence presently favouring deleterious effects of EVs in asthma. Numerous studies highlighted differences in exosomal contents between EVs of healthy and asthmatic individuals, which could be employed as potential diagnostic markers. In some circumstances, EVs were also found to be suppressive to disease, but more often promote inflammation and airway remodelling. In conclusion, EVs hold immense potential in understanding the pathophysiology of asthma, and as diagnostic and therapeutic markers. While more research is needed for definitive conclusions and their application in medical practice, the literature presented in this review should encourage further research and discovery within the field of EVs and asthma.

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Comparative proteomics of respiratory exosomes in cystic fibrosis, primary ciliary dyskinesia and asthma

Cited by 39 publications

References 44 publications

Increased extracellular vesicles mediate inflammatory signalling in cystic fibrosis

Increased extracellular vesicles mediate inflammatory signalling in cystic fibrosis

Bone-Derived Extracellular Vesicles: Novel Players of Interorgan Crosstalk

Extracellular vesicles and asthma: A review of the literature

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