Comparative Proteomics of Whey and Milk Fat Globule Membrane Proteins of Guanzhong Goat and Holstein Cow Mature Milk

Sun, Yuxue; Wang, Cuina; Sun, Xiaomeng; Guo, Mingruo

doi:10.1111/1750-3841.14428

Cited by 42 publications

(47 citation statements)

References 39 publications

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“…Final digested samples were filtered using a 10 kDa ultra‐centrifugal filter to obtain the peptide mixtures, followed by desalting using a C18 stage tip. The desalted peptides were separated by Thermo Fisher easy nano‐LC 1000 system (Thermo Scientific) and characterized on a Q‐Exactive (Thermo Scientific) as previously described . In brief, the desalted peptides were loaded on a pre‐column (100 μm × 2 cm, 5 μm, C18 particle) and followed by an analytical column (75 μm × 100 nm, 3 μm, C18 particle).…”

Section: Methodsmentioning

confidence: 99%

“…The desalted peptides were separated by Thermo Fisher easy nano-LC 1000 system (Thermo Scientific) and characterized on a Q-Exactive (Thermo Scientific) as previously described. 18 In brief, the desalted peptides were loaded on a pre-column (100 μm × 2 cm, 5 μm, C18 particle) and followed by an analytical column (75 μm × 100 nm, 3 μm, C18 particle). Mobile phase A and B consisted of 0.1% formic acid in water and in 80% acetonitrile, respectively.…”

Section: Peptides Identification By Easy-nlc-orbitrap Ms/msmentioning

confidence: 99%

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Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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BACKGROUND Xinong Saanen goat milk is a raw material for goat milk‐based infant formula production. This study aims to analyze digestion properties of Xinong Saanen goat colostrum and mature milk by simulating infant gastrointestinal digestion. Zeta potential, particles size, protein profile and peptides composition of these two kinds of milk during the digestion process were studied. RESULTS Zeta‐potential values of the digested colostrum were lower than those of mature milk through the whole digestion. Absolute zeta potential of colostrum duodenal digestion samples showed a decrease from 16.63 ± 2.08 to 11.80 ± 2.03 mV while that of mature milk decreased sharply and then increased (P < 0.05). Colostrum had a larger particle size than mature milk and both milks showed decreased particle size with increasing digestion time but an increase for the last 30 min. Colostrum showed more high molecular weight (MW) proteins which cannot be hydrolyzed completely compared with mature milk. Digested peptides (< 10 kDa) were characterized using liquid chromatography combined with tandem mass spectrometry (LC–MS/MS). The casein‐derived peptides identified in digested colostrum and mature milk accounted for 76.67% and 59.53%, respectively. β‐Casein was the most abundant in colostrum while that in mature milk was αs1‐casein. Enterotoxin‐binding glycoprotein PP20K, butyrophilin subfamily 1 member A1 (BTN1A1) and perilipin (PLIN) were only detected in digested mature milk. CONCLUSION Differences in digestion properties between goat colostrum and mature milk were mainly shown in duodenal digestion phase. Data may provide useful information about utilization of goat milk for infant formula formulation. © 2019 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Peptides Identification By Easy-nlc-orbitrap Ms/msmentioning

confidence: 99%

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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“…This important information helped identify the main proteins with immunity-promoting properties for newborns [66]. These analyses have been performed to study and compare the proteome of various MFGMs, mainly from humans and cows [64][65][66], but also from other species such as the goat, yak, buffalo, horse, and donkey [61,62,[67][68][69][70].…”

Section: Comparison Of Mfgm Proteome Between Different Species and Lamentioning

confidence: 99%

“…In a 2019 study, a total of 776 MFGM proteins were identified: 427 and 183 that are unique for goat and cow milk, respectively, and 166 proteins shared between the two species. Most of the goat MFGM proteins were related to metabolic processes (about 21%), whereas most of the cow MFGM proteins were related to disease-associated pathways (about 49%) [62]. Subsequently, the same authors evaluated the variations between goat colostrum and mature MFGM proteome.…”

Section: Comparison Of Mfgm Proteome Between Different Species and Lamentioning

confidence: 99%

Comparative Proteomics of Milk Fat Globule Membrane (MFGM) Proteome across Species and Lactation Stages and the Potentials of MFGM Fractions in Infant Formula Preparation

Manoni

Lorenzo

Ottoboni

et al. 2020

Foods

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Milk is a lipid-in-water emulsion with a primary role in the nutrition of newborns. Milk fat globules (MFGs) are a mixture of proteins and lipids with nutraceutical properties related to the milk fat globule membrane (MFGM), which protects them, thus preventing their coalescence. Human and bovine MFGM proteomes have been extensively characterized in terms of their formation, maturation, and composition. Here, we review the most recent comparative proteomic analyses of MFGM proteome, above all from humans and bovines, but also from other species. The major MFGM proteins are found in all the MFGM proteomes of the different species, although there are variations in protein expression levels and molecular functions across species and lactation stages. Given the similarities between the human and bovine MFGM and the bioactive properties of MFGM components, several attempts have been made to supplement infant formulas (IFs), mainly with polar lipid fractions of bovine MFGM and to a lesser extent with protein fractions. The aim is thus to narrow the gap between human breast milk and cow-based IFs. Despite the few attempts made to date, supplementation with MFGM proteins seems promising as MFGM lipid supplementation. A deeper understanding of MFGM proteomes should lead to better results.

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“…Many knowledge domains have successfully implemented and benefitted from ontologies for knowledge management including Genes analysis [9], chemical assessment framework [10], dairy industry [11], healthcare [12,13], crises management [14], the music industry [15], agriculture [16], and so forth.…”

Section: A Ontology: State Of the Artmentioning

confidence: 99%

A Semantic Ontology for Disaster Trail Management System

Ahmad¹,

Othman²,

Fauzan³

et al. 2019

IJACSA

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Disasters, whether natural or human-made, leave a lasting impact on human lives and require mitigation measures. In the past, millions of human beings lost their lives and properties in disasters. Information and Communication Technology provides many solutions. The issue of so far developed disaster management systems is their inefficiency in semantics that causes failure in producing dynamic inferences. Here comes the role of semantic web technology that helps to retrieve useful information. Semantic web-based intelligent and self-administered framework utilizes XML, RDF, and ontologies for a semantic presentation of data. The ontology establishes fundamental rules for data searching from the unstructured world, i.e., the World Wide Web. Afterward, these rules are utilized for data extraction and reasoning purposes. Many disaster-related ontologies have been studied; however, none conceptualizes the domain comprehensively. Some of the domain ontologies intend for the precise end goal like the disaster plans. Others have been developed for the emergency operation center or the recognition and characterization of the objects in a calamity scene. A few ontologies depend on upper ontologies that are excessively abstract and are exceptionally difficult to grasp by the individuals who are not conversant with theories of the upper ontologies. The present developed semantic web-based disaster trail management ontology almost covers all vital facets of disasters like disaster type, disaster location, disaster time, misfortunes including the causalities and the infrastructure loss, services, service providers, relief items, and so forth. The objectives of this research were to identify the requirements of a disaster ontology, to construct the ontology, and to evaluate the ontology developed for Disaster Trail Management. The ontology was assessed efficaciously via competency questions; externally by the domain experts and internally with the help of SPARQL queries.

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Comparative Proteomics of Whey and Milk Fat Globule Membrane Proteins of Guanzhong Goat and Holstein Cow Mature Milk

Cited by 42 publications

References 39 publications

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

Comparative Proteomics of Milk Fat Globule Membrane (MFGM) Proteome across Species and Lactation Stages and the Potentials of MFGM Fractions in Infant Formula Preparation

A Semantic Ontology for Disaster Trail Management System

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