Comparative Studies on Aluminum Tolerance Screening Techniques for Sorghum, Soybean and Maize in Simple Solution Culture

Akhter, Afrin; Wagatsuma, Tadao; Khan, Muhammad Shuaib; Tawaraya, Keitarou

doi:10.3923/ajpp.2009.1.8

Cited by 8 publications

(8 citation statements)

References 1 publication

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“…For the development of tolerant cultivars, genotypes with favorable characteristics for cultivation under Al stress must be identified. Considering that Al directly affects the root system, the hydroponic cultivation is advantageous for interaction studies of this element with plants, since it enables free access to the root system and the possibility of monitoring and controlling pH, Al concentrations and other ions that are relevant for the expression of sensitivity or tolerance responses [14,15]. To this end, the Al concentrations in nutrient solution at which the genotypes can be efficiently discriminated must be determined.…”

Section: Introductionmentioning

confidence: 99%

Response of Common Bean Cultivars and Lines to Aluminum Toxicity

et al. 2020

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The soils in the common bean-producing regions (Phaseolus vulgaris L.) of Brazil are usually acid and conta\y66\yin toxic levels of aluminum (Al) for plants. This ion causes yield losses by inhibiting root cell expansion, thus reducing water and nutrient uptake. This study investigates the optimal Al concentration for the screening of genotypes in hydroponics cultivation and tries to identify cultivars and lines for cultivation in Al-toxic soils. The study consisted of two series of experiments. In the first one, four cultivars were evaluated at five Al concentrations (0, 2.5, 5, 7.5 and 10 ppm) and in the second, four independent tests were carried out (1-carioca, 2-black, 3-red, and 4-white), each with seven genotypes and two Al concentrations (0 and 4 ppm). The optimized concentration of Al in the first stage was 4 ppm, which allowed the early identification of genotypes with less affected development under Al toxicity in the second stage. The common bean cultivars IPR Quero-Quero (carioca group), BRS Esplendor (black group), KID 44 (red group), and WLine 5 (white group) may be indicated for cultivation under Al toxicity.

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Section: Introductionmentioning

confidence: 99%

Response of Common Bean Cultivars and Lines to Aluminum Toxicity

et al. 2020

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“…Pea ( Pisum sativum L.), being a legume species, may be considered as a relatively Al-sensitive crop as compared to cereals [ 11 , 17 , 18 , 19 ]. A vulnerability of leguminous plants in terms of Al toxicity is a high sensitivity in the formation of symbiosis with microorganisms, particularly with nitrogen-fixing nodule bacteria [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

Belimov

Шапошников

Syrova

et al. 2020

Plants

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Aluminium being one of the most abundant elements is very toxic for plants causing inhibition of nutrient uptake and productivity. The aim of this study was to evaluate the potential of microbial consortium consisting of arbuscular mycorrhizal fungus (AMF), rhizobia and PGPR for counteracting negative effects of Al toxicity on four pea genotypes differing in Al tolerance. Pea plants were grown in acid soil supplemented with AlCl3 (pHKCl = 4.5) or neutralized with CaCO3 (pHKCl = 6.2). Inoculation increased shoot and/or seed biomass of plants grown in Al-supplemented soil. Nodule number and biomass were about twice on roots of Al-treated genotypes after inoculation. Inoculation decreased concentrations of water-soluble Al in the rhizosphere of all genotypes grown in Al-supplemented soil by about 30%, improved N2 fixation and uptake of fertilizer 15N and nutrients from soil, and increased concentrations of water-soluble nutrients in the rhizosphere. The structure of rhizospheric microbial communities varied to a greater extent depending on the plant genotype, as compared to soil conditions and inoculation. Thus, this study highlights the important role of symbiotic microorganisms and the plant genotype in complex interactions between the components of the soil-microorganism-plant continuum subjected to Al toxicity.

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“…Ranking according to the damaged tissue staining intensity in some samples was difficult, despite the fact that the length of the root re-growth zone was generally greater in the samples with lower hematoxylin staining intensity (data not shown). This is probably due to the pea species characteristics, as in other plant species hematoxylin was successfully used to differentiate varieties by aluminum resistance by assessing the root staining intensity, and this figure was correlated with aluminum resistance growth rates [4,19,20,22,36]. Root treatment with eriochrome cyanine R revealed high variability of pea samples in staining intensity, and the root re-growth zone was visually distinguishable.…”

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confidence: 91%

“…In stable samples, aluminum ions are either eliminated from tissues actively or bound in complexes that do not react with the dye, and probably do not have biological activity [20]. This approach has been successfully applied to assess the intra-specific aluminum tolerability variation in soybean [4], corn [4,19], wheat [21], and sorghum [4,22]. However, this method has been mentioned [5,23,24] to have significant disadvantages which include subjective assessment of resistance using a point system; uneven hematoxylin washout from roots; in each experiment, the need to include genotypes that are the standards of root coloration; no staining in most aluminum tolerable genotypes.…”

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confidence: 99%

“…According to the Ministry of Agriculture of the Russian Federation data as of January 1, 2011, acidic soils make up 30.2 million ha or 35.6 % of the examined 84.6 million hectares of tillage [1]. Crops vary considerably in their resistance to acidic soils, and many legumes (garden pea, lentils, and nut) are considered to be sensitive or moderately sensitive crops compared to cereals (rye, oats, triticale, rice, wheat, and barley) and corn [2][3][4][5]. Despite its sensitivity to toxic aluminum ions, pea plants, in some degree, can be resistant to soil acidity [3,6].…”

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confidence: 99%

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METHOD FOR RAPID ASSESSMENT OF ALUMINUM TOLERANCE OF PEA (Pisum sativum L.)

Vishnyakova¹,

Семенова²,

Kosareva³

et al. 2015

S-h. biol.

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A b s t r a c tCrops vary considerably in their resistance to acidic soils, and many legumes, including pea (Pisum sativum L.), considered to be sensitive or moderately sensitive crops compared to cereals. The main factor determining the phytotoxicity of acidic soils is the increased concentration of mobile aluminum ions in the soil solution. Accumulation of aluminum in root tissues interferes with cell division, initiation of growth of lateral roots and uptake of minerals and water by plants. Under laboratory conditions the resistance of plants to aluminum is estimated by the degree of damage to the roots by aluminum using dyes (hematoxylin, eriochrome cyanine R) and the ability of roots to restore growth after toxic effect of this metal. This work dedicated to the development of rapid assessment of aluminum tolerance especially for peas, which is as follows: the seeds were germinated in the growth chamber in the nutrient solution for 3 days (7000 lx illumination, temperature of 19 С at night and 21 С during the day, photoperiod 16 h), treated with a toxic concentration of aluminum chloride (3 mg Al/l) for 24 hours, incubated in fresh nutrient solution without aluminum for 2 days and stained with 0.1 % eriochrome cyanine R for 10 min. Zone of root tissue damage by aluminum painted in the color purple. Plant resistance to aluminum was determined by the length of the root re-growth area after exposure to the toxicant. Using 19 varieties of pea from the N.I. Vavilov Research Institute of Plant Industry collection (VIR collection) it was shown that pea has high variability in tolerance to aluminum. Varieties with a minimum (1.01.5 mm) length of the root re-growth (k-2759, k-3654 and k-3283) were characterized by intense purple color of the root, but varieties with a maximum (14.014.5 mm) length of the root re-growth (k-4376, k-9504 and k-7307) had a faint but detectable staining. The proposed method makes it possible to identify genotypes contrasting in aluminum tolerance, is highly reproducible and can be used for screening and study of intraspecific variability in this trait of pea plants at very early developing stage.

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Comparative Studies on Aluminum Tolerance Screening Techniques for Sorghum, Soybean and Maize in Simple Solution Culture

Cited by 8 publications

References 1 publication

Response of Common Bean Cultivars and Lines to Aluminum Toxicity

Response of Common Bean Cultivars and Lines to Aluminum Toxicity

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

METHOD FOR RAPID ASSESSMENT OF ALUMINUM TOLERANCE OF PEA (Pisum sativum L.)

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