Comparative Studies on the Oxidative Processes During Phagocytosis Measured by Luminol-Dependent Chemiluminescence

Bruchelt, Gernot; Schmidt, Karl‐Heinz

doi:10.1515/cclm.1984.22.1.1

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…The increased CL in the Percoll preparations seems more likely to be due to non-specific priming by the Percoll beads. It is possible that the polyvinylpyrrolidonecoated silica particles from the Percoll initiate perturbation of the cell membrane as noted by Bruchelt and Schmidt (1984). However, the reason for using Percoll gradients is that this reagent seemed to have the mildest effect, giving the lowest CL in our unpublished studies comparing Ficoll-Hypaque (Pharmacia, Uppsala, Sweden) and Mono-Poly (Flow Laboratories, North Ryde, Australia), which is in agreement with the findings of Venaille et al (1994).…”

Section: Discussionsupporting

confidence: 85%

Periodontitis increases chemiluminescence of the peripheral neutrophils independently of priming by the preparation method

et al. 1999

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OBJECTIVE: The aim was to investigate whether increased Fcγ‐receptor (FcγR)‐induced chemiluminescence (CL) from peripheral neutrophils in periodontitis could be due to increased sensitivity to non‐specific priming by the cell preparation. DESIGN: Two methods of preparation were used: (1) cells purified by density centrifugation and lysis of erythrocytes; and (2) cells in a leukocyte suspension obtained by lysis of erythrocytes alone. METHODS: Neutrophils from 17 subjects with periodontitis and pair‐matched healthy controls were studied by CL and flow cytometry (CD16, CD15 and CD11b). RESULTS: Higher CL was found in the purified neutrophils, indicating higher non‐specific priming by the Percoll density centrifugation. Priming with TNFα and lipo‐polysaccharide abolished this difference. The net effect of specific priming was significantly lower in the purified neutrophils, indicating a depletion of the priming sensitivity by the density centrifugation. Both preparations reduced CD15 in relation to whole blood, while there was no difference between the two methods in any of the membrane markers. Thus, in both preparations, lysis alone changed the membranes to the same extent. Both methods gave nearly the same relative CL increase in the periodontitis patients as in the controls. CONCLUSION: There was no difference between the neutrophils from patients and controls in sensitivity to non‐specific priming by the preparation method. The higher FcγR‐induced CL in patients with periodontitis is not related to such a non‐specific priming by density centrifugation.

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Section: Discussionsupporting

confidence: 85%

Periodontitis increases chemiluminescence of the peripheral neutrophils independently of priming by the preparation method

et al. 1999

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“…We measured CL responsiveness of arterial blood from catheterized dogs at a maximum dilution of 1:2.5 by applying our routine method for detection of CL in isolated human leukocytes (Wilhelms, 1987). We did not observe a marked suppression of CL in relation to the number of accompanying erythrocytes in the samples (Bruchelt, 1984) instead we found CL increased almost linearly and inversely to blood dilution (Figs 1 and 2).…”

Section: Resultscontrasting

confidence: 51%

“…Chemiluminescence (CL) has been studied as an indicator for (1) leukocyte activation (LA) and (2) the formation of pro-inflammatory 'excited oxygen species'. Isolated leukocytes, and diluted blood samples have been used for such investigations (Allen et al, 1985;Bruchelt and Schmidt, 1984;Kato et al, 1981. The aim of this study was to establish suitable conditions for a reproducible test utilizing zymosan (C,-Z) stimulated, luminol-amplified CL of minimally diluted blood samples from beagle *Author for correspondence. 0884-3996/90/030197-06$0.5 .OO @ 1990 by John Wiley & Sons, Ltd dogs.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence (CL) in blood samples reflects leukocyte activation (LA) during anaphylactoid reactions in dogs

Wilhelms

1990

J. Biolumin. Chemilumin.

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Inflammation is accompanied by leukocyte activation (LA). We describe a simple ex vivo technique for studying LA that might help to find new LA inhibitors for the treatment of pathologic events related to LA. Arterial and venous blood samples obtained from six permanently catheterized beagle dogs -60, 0, +15 min and +23 h after i.v. challenge with C 48/80, and also blood samples from six normal beagles, were minimally diluted 1:2.5 with buffer. Total leukocyte counts (LC), and luminol amplified CL, induced by opsonized zymosan (C3-Z), were estimated. Blood samples from dogs elicited CL responses of almost 1/10 the magnitude of erythrocyte-free human leukocytes, whereas blood samples from rats reacted three orders of magnitude less. Obviously quenching of CL by accompanying erythrocytes in blood samples from dogs is not important, for CL correlated almost linearly with the CL in differently diluted samples. In arterial, but not in venous samples from catheterized dogs, absolute CL and LC, both were significantly depressed (p less than or equal to 0.05) 15 min after C 48/80 challenge. CL/10(6) leukocytes was augmented twofold. All leukocyte deviations returned to pre-values 23 h post-challenge.

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“…Zymosan (Merck, Darmstadt, FRG) was treated with plasma as described previously (1). In short, 0.2 g zymosan were homogenized and suspended in 20 mL NaCl solution (1 50 mmol/L), then stirred for 20 h. After spinning, the pellet was resuspended in lOmL plasma freshly prepared from human blood and incubated for 30min at 37°C.…”

Section: Opsonization Of Zymosanmentioning

confidence: 99%

Luminol‐enhanced chemiluminescence after reaction of hydroperoxides with opsonized zymosan

Heinle¹,

Dessouki²

1995

J. Biolumin. Chemilumin.

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Luminol-enhanced chemiluminescence (LEC) is very sensitive in detecting free radicals but relatively insensitive for hydroperoxides (hydrogen peroxide, tert-butyl hydroperoxide). However, in the presence of opsonized zymosan (often used for stimulation of phagocytic cells) hydroperoxides also induce LEC, suggesting that free radicals are produced under these conditions. Therefore careful interpretation with respect to the nature of the reactive species is necessary when LEC is used for characterization of zymosan-induced phagocytosis. We studied the properties of zymosan-induced LEC under different test conditions and with various inhibitors. Typical radical scavengers, e.g. nordihydroguaiaretic acid and superoxide dismutase, are strong inhibitors, indicating the importance of the superoxide anion. This system is useful for drug testing with respect to antioxidative or radical scavenging activity.

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Comparative Studies on the Oxidative Processes During Phagocytosis Measured by Luminol-Dependent Chemiluminescence

Cited by 10 publications

References 14 publications

Periodontitis increases chemiluminescence of the peripheral neutrophils independently of priming by the preparation method

Periodontitis increases chemiluminescence of the peripheral neutrophils independently of priming by the preparation method

Chemiluminescence (CL) in blood samples reflects leukocyte activation (LA) during anaphylactoid reactions in dogs

Luminol‐enhanced chemiluminescence after reaction of hydroperoxides with opsonized zymosan

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