2004
DOI: 10.1016/j.bios.2003.10.010
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Comparative study of electron mediators used in the electrochemical oxidation of NADH

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Cited by 93 publications
(68 citation statements)
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“…These low values indicate very slow kinetics of the NAD + reduction reaction on a glassy carbon electrode and reflect the over potential necessary for the redox reaction which is related to both redox kinetics and mass transport. One strategy employed to overcome these difficulties (fouling and overvoltage and side reactions) was the use of mediator-modified electrodes, where the mediators are used to shuttle electrons from NADH to the electrode surface and allow electron transport between them [5,[9][10][11][12][13][14][15][16]. Some mediators (electrocatalysts) were immobilized on the electrode surface by covalent attachment, electrochemical polymerization, incorporation in carbon paste, adsorption, self-assembly and via entrapment in polymeric matrices [4,[17][18][19].…”
Section: David Publishingmentioning
confidence: 99%
“…These low values indicate very slow kinetics of the NAD + reduction reaction on a glassy carbon electrode and reflect the over potential necessary for the redox reaction which is related to both redox kinetics and mass transport. One strategy employed to overcome these difficulties (fouling and overvoltage and side reactions) was the use of mediator-modified electrodes, where the mediators are used to shuttle electrons from NADH to the electrode surface and allow electron transport between them [5,[9][10][11][12][13][14][15][16]. Some mediators (electrocatalysts) were immobilized on the electrode surface by covalent attachment, electrochemical polymerization, incorporation in carbon paste, adsorption, self-assembly and via entrapment in polymeric matrices [4,[17][18][19].…”
Section: David Publishingmentioning
confidence: 99%
“…This approach reduces fouling problems which represents an important improvement compared to the entrapment strategy. However, this approach generates poor sensitivities and low reproducibility (Prieto-Simón and Fàbregas 2004). Finally, the mediator was immobilized by electropolymerization on epoxy-graphite electrode surfaces.…”
Section: Comparison Of Processes With Dissolved and Immobilized Mediamentioning
confidence: 99%
“…Finally, the mediator was immobilized by electropolymerization on epoxy-graphite electrode surfaces. Among all these strategies of including a mediator in the amperometric analysis of NADH, the electropolymerization showed the best results regarding the reproducibility of consecutive calibration curves (Prieto-Simón and Fàbregas 2004). Thus, this approach appeared to be the best solution to the problems They tested (a) the direct mixing of MB in the screen-printing ink, (b) incorporation of MB after precipitation with Reinecke salt (MBRS), and (c) electrodeposition of a MB-derived polymer (poly(MB)) on the surface of graphite electrodes.…”
Section: Comparison Of Processes With Dissolved and Immobilized Mediamentioning
confidence: 99%
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“…No sistema aqui proposto, o NADH produzido na reação será reoxidado usando o MBRS como um aceptor de elétrons, presente em um eletrodo de grafite (Figura 1). Essa estratégia tem sido utilizada em vários processos que empregam enzimas NAD + -dependentes [27][28][29] . O princípio de detecção do biossensor será baseado na inibição irreversível da enzima AldH, inibição esta proporcional à concentração dos DTC's.…”
Section: Introductionunclassified