Comparative study of hydrophobically modified gelatin‐based sealant with commercially available sealants

Abstract

Air leakage is one of the major complications related to pulmonary surgeries. To reduce this complication, we developed a decyl group (C10)‐modified Alaska pollock gelatin (ApGltn) (C10‐ApGltn) sealant and evaluated its practical performance against commercially available sealants, Beriplast® and DuraSeal®. C10‐ApGltn was synthesized by reductive amination of the amino groups in ApGltn with decanal. C10‐ApGltn was crosslinked with a poly(ethylene glycol)‐based crosslinker to form a tissue sealant. The crosslinking time of the C10‐ApGltn sealant was fast enough for curing on tissue and application as a spray system. Although the percent swelling of C10‐ApGltn and DuraSeal was significantly greater than Beriplast, C10‐ApGltn and DuraSeal exhibited excellent tissue sealing properties on pleura tissue under a long‐term moist condition. Additionally, C10‐ApGltn and DuraSeal did not cause severe inflammatory responses in a rat subcutaneous example. Therefore, C10‐ApGltn sealant had comparable tissue sealing properties to DuraSeal under a moist condition, indicating the potential of C10‐ApGltn sealant for pulmonary surgeries.

Improved Swelling Property of Tissue Adhesive Hydrogels Based on α‐Cyclodextrin/Decyl Group‐Modified Alaska Pollock Gelatin Inclusion Complexes

“…We have developed a surgical adhesive consisting of alkyl groups-modified Alaska pollock-derived gelatin (ApGltn) and a PEG-based crosslinker, pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG). [15][16][17][18][19][20][21][22][23] Introduced alkyl groups effectively enhanced adhesion strength on the tissue surfaces when the degree of substitution (DS) of alkyl groups was ≈10 mol%. [15,21] However, cured adhesives swelled over 200% after immersion in saline.…”