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Air leakage is one of the major complications related to pulmonary surgeries. To reduce this complication, we developed a decyl group (C10)‐modified Alaska pollock gelatin (ApGltn) (C10‐ApGltn) sealant and evaluated its practical performance against commercially available sealants, Beriplast® and DuraSeal®. C10‐ApGltn was synthesized by reductive amination of the amino groups in ApGltn with decanal. C10‐ApGltn was crosslinked with a poly(ethylene glycol)‐based crosslinker to form a tissue sealant. The crosslinking time of the C10‐ApGltn sealant was fast enough for curing on tissue and application as a spray system. Although the percent swelling of C10‐ApGltn and DuraSeal was significantly greater than Beriplast, C10‐ApGltn and DuraSeal exhibited excellent tissue sealing properties on pleura tissue under a long‐term moist condition. Additionally, C10‐ApGltn and DuraSeal did not cause severe inflammatory responses in a rat subcutaneous example. Therefore, C10‐ApGltn sealant had comparable tissue sealing properties to DuraSeal under a moist condition, indicating the potential of C10‐ApGltn sealant for pulmonary surgeries.

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Adhesives/sealants are used after suturing to prevent leakage of cerebrospinal fluid from an anastomotic site. Commercial adhesives/sealants have been used to close the cerebral dura. However, swelling of the cured adhesives/sealants induces increased intracranial pressure and decreases the strength of the seal. In the present study, tissue adhesive hydrogels with improved swelling property using inclusion complex composed of 𝜶-cyclodextrin (𝜶CD) and decyl group (C10)-modified Alaska pollock-derived gelatin (C10-ApGltn) with a high degree of substitution (DS) (>20 mol%) are developed. Viscosity of C10-ApGltn with a high DS solution remarkably decreased by the addition of 𝜶CD. The resulting 𝜶CD/C10-ApGltn adhesive hydrogel composed of 𝜶CD/C10-ApGltn inclusion complexes and poly(ethylene glycol) (PEG)-based crosslinker showed improved swelling property after immersion in saline. Also, the resulting adhesive has a significantly higher burst strength than fibrin-based adhesives and is as strong as a PEG-based adhesive. Quantitative analysis of 𝜶CD revealed that the improved swelling property of the resulting adhesive hydrogels is induced by the release of 𝜶CD from cured adhesive, and the subsequent assembly of decyl groups in the saline. These results suggest that adhesives developed using the 𝜶CD/C10-ApGltn inclusion complex can be useful for closing the cerebral dura mater.