Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Li, Peijun; Xia, Jin-lan; Shan, Yang; Nie, Zhen-yuan

doi:10.1007/s00449-015-1442-3

Cited by 29 publications

(13 citation statements)

References 43 publications

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“…In particular, depending on the studied microorganism ( Aspergillus spp., Trichoderma sp.) and fermentation substrates (flax seed fibers, wheat bran), as well as the parameters of sonication, the enzyme activities in the extracts obtained were increased up to four times .…”

Section: Introductionmentioning

confidence: 99%

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and α-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity (ࣙ7.5 IU).

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Section: Introductionmentioning

confidence: 99%

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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“…In a previous report [ 7 ], A. japonicus PJ01 was used for multi-enzyme complexes (MEC) production under solid-state fermentation (SSF). In the extracellular extracts, pectinase, CMCase, and xylanase activities were 48.3 ± 0.2, 2.9 ± 0.1, and 50.2 ± 0.2 U/mL, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…According to our previous report [ 7 ], the main organic matter in the extracellular extracts was composed of soluble proteins, reducing sugars, and polysaccharides. The soluble proteins stem from the fungal metabolites (enzymes and proteins) and wheat bran.…”

Section: Discussionmentioning

confidence: 99%

“…Fungal biomass or metabolites is usually harvested in submerged fermentation (SmF). Compared to SmF, solid-state fermentation (SSF) presents unparalleled advantages, such as higher productivity per reactor volume, lower capital and operating costs, lower space requirements, simpler equipment, and easier downstream processing [ 7 ]. However, most fungal extracellular extracts used for biogenic synthesis of AgNPs were derived from SmF; few reports focused on SSF.…”

Section: Introductionmentioning

confidence: 99%

“…Except for microbial enzymes, reducing sugars and polysaccharides are also the main components of fungal extracellular extracts, which can be used as reducing agents or stabilizers in the synthesis of metal nanoparticles. In our previous report [ 7 ], A. japonicus PJ01 was used for multi-enzyme complexes (MEC) production under SSF, and the MEC contained pectinase, CMCase, and xylanase. As far as we know, there were few reports on the preparation of AgNPs by the fungal multi-enzyme complexes, and the reaction mechanism remained to be solved.…”

Section: Introductionmentioning

confidence: 99%

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Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Pan

Tao

et al. 2021

Molecules

Self Cite

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The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.

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Multi‐enzymatic recovery of fungal cellulases (Aspergillus niger) through solid‐state fermentation of sugarcane bagasse

et al. 2021

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In this study, the optimum conditions for the multi‐enzymatic recovery of cellulases produced by Aspergillus niger were investigated using sugarcane bagasse. Two extraction methods were investigated: the two‐stage solid–liquid extraction (SLE) followed by ultrasound‐assisted extraction (UAE), and the single‐stage SLE. The ultrasound effects were evaluated using a Doehlert design, in which the pH (5.0–9.0) and sonication power (0.8–2.0 W ml−1) were independent variables. For the single‐stage SLE, temperature (25–45°C), time (10–60 min), and pH (5.0–9.0) were analyzed using the Box–Behnken design. Both processes were monitored to evaluate FPase, CMCase, and β‐glucosidase (U ml−1) activities. The maximum enzymatic activities (EA) obtained for FPase, CMCase, and β‐glucosidase in the SLE–UAE were 0.352, 0.321, and 1.412 U ml−1, respectively. Unlike in most previous studies, sonication was insignificant (p < 0.05) with respect to the enzymatic complex within the evaluated ranges. Moreover, sonication changed the EA when lower than 1.2 and higher than 1.6 W ml−1, mainly inhibiting the EA of β‐glucosidase. The single‐stage SLE was more effective than the two‐stage SLE–UAE, and the maximum EA values for FPase, CMCase, and β‐glucosidase were 0.354, 0.303, and 3.135 U ml−1, respectively. The single‐stage process was better because it consumed less energy, required simpler equipment, and provided higher efficiency in a shorter time. This study will improve diversified enzyme extraction from sugarcane bagasse, reduce enzyme production costs, and enhance bagasse utilization.

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Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Cited by 29 publications

References 43 publications

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Multi‐enzymatic recovery of fungal cellulases (Aspergillus niger) through solid‐state fermentation of sugarcane bagasse

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