Comparative study of silanisation reactions for the biofunctionalisation of Ti-surfaces

Dubruel, Peter; Vanderleyden, Els; Bergada, M; Paepe, Ilse De; Chen, H; Kuypers, S.; Luyten, Jan; Schrooten, Jan; Hoorebeke, Luc Van; Schacht, Etienne

doi:10.1016/j.susc.2006.04.021

Cited by 39 publications

(25 citation statements)

References 32 publications

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“…The pre-treatment and silanisation of the Ti samples was performed as described previously [28]. In brief, the Ti samples were degreased and the native oxide layer was etched before treatment with an oxidizing agent.…”

Section: Development Of Gelatin-coated Titaniummentioning

confidence: 99%

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Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization

et al. 2011

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Abstract:In the present work, the immobilization of gelatin as biopolymer on two types of implantable biomaterials, polyimide and titanium, was compared. Both materials are known for their biocompatibility while lacking cell-interactive behavior. For both materials, a pre-functionalization step was required to enable gelatin immobilization. For the polyimide foils, a reactive succinimidyl ester was introduced first on the surface, followed by covalent grafting of gelatin. For the titanium material, methacrylate groups were first introduced on the Ti surface through a silanization reaction. The applied functionalities enabled the subsequent immobilization of methacrylamide modified gelatin. Both surface modified materials were characterized in depth using atomic force microscopy, static contact angle measurements, confocal fluorescence microscopy, attenuated total reflection infrared spectroscopy and X-ray photo-electron spectroscopy. The results indicated that the strategies elaborated for both material classes are suitable to apply stable gelatin coatings. Interestingly, depending on the material class studied, not all surface analysis techniques are applicable.

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Section: Development Of Gelatin-coated Titaniummentioning

confidence: 99%

“…Ti has gained large interest as a load-bearing scaffold material for bone tissue engineering [28][29][30]. In order to realize bone healing, osseointegration of the metal implant in the surrounding tissue has to be achieved.…”

Section: Introductionmentioning

confidence: 99%

Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization

et al. 2011

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“…[12][13][14][15][16] In a previous paper, we reported on the application of different silanisation procedures and silanisation reagents for the functionalisation of Ti surfaces. [17] We showed that a methacrylate modified Ti surface was an appropriate starting material for the subsequent bio-activation of Ti surfaces. In that study, we applied the covalent immobilisation of gelatin on the Ti surface as proof of concept.…”

Section: Introductionmentioning

confidence: 97%

Comparative Study of Collagen and Gelatin Coatings on Titanium Surfaces

Vanderleyden

Hoorebeke

Schacht

et al. 2011

Macromolecular Symposia

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To date, critical bone defects are mainly treated by implantation of a bio-inert metal such as titanium (Ti) or nickel-titanium alloys. The surface of such biomaterials is in direct contact with the host tissue and thus plays a critical role in determining the biocompatibility. There are several reports indicating that osteointegration of these implants is not optimal. In order to improve the integration of implants, it is desirable to control interfacial reactions such that tissue-healing phenomena can be controlled.1 Our goal is to apply natural polymers as stable bio-active coatings for porous Ti scaffolds. To achieve such a stable polymeric coating, we have applied a four-step-procedure: (1) cleaning, (2) oxidation and (3) silanisation of the titanium surface followed by (4) polymer immobilisation (figure 1).In the present work we compared the immobilisation of collagen type I with the immobilisation of its hydrolysed derivative, gelatin. Collagen is one of the most usefull biomaterials due to its excellent biocompatibility, biodegradability and weak antigenicity. Still, a more economical choice would be gelatin. Collagen was immobilised onto the Ti surface in an adsorptive and in a covalent manner. 2,3 Only the latter proved to lead to a stable coating. This method comprised of a silanisation with 3-aminopropyltriethoxysilane (APTES) to introduce amines to the Ti surface. In a next step, these amines can react with the carboxylic acid groups of collagen by using a coupling reagent. Without any doubt, there will also be intra-and intermolecular crosslinking between the collagen chains. In case of gelatin, we followed a different approach. Gelatin was modified with metacrylamide groups (Gel-mod) and the Ti surface was modified with methacrylate groups through silanisation using 3-(trimethoxysilyl)propyl methacrylate (TMSPMA). Crosslinking between both was achieved by e-beam treatment.

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“…To biologically modify metal surfaces, silane-based coupling methods have been conventionally employed to prepare an initial organic layer on the metal surface. [7][8][9][10] However, in addition to physicochemical modification, [11][12][13] recent biomimetic approaches inspired by underwater organisms for surface modification have been proposed by many studies.…”

Section: Introductionmentioning

confidence: 99%

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

Zhou¹,

Park²,

Mao³

et al. 2015

IJN

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Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31 P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

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Comparative study of silanisation reactions for the biofunctionalisation of Ti-surfaces

Cited by 39 publications

References 32 publications

Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization

Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization

Comparative Study of Collagen and Gelatin Coatings on Titanium Surfaces

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

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