Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum

NITROGEN SOURCES ON TPOMW VALORIZATION THROUGH SOLID STATE FERMENTATION PERFORMED BY Yarrowia lipolytica

“…In the first 24 hours, the productivity reached its best range with 7.8 U/g*h, and after four days of the process only decreased about 35%. This promising result confirms the potential of our experiments since the lipase productivity was higher than most of the productivities reported in the literature (Imandi et al, 2010;Moftah et al, 2012;Coradi et al, 2012;Vaseghi et al, 2013;Salgado et al, 2013), which is very important for an economically viable process. Although the organic nitrogen sources are generally cited for lipase production, the use of inorganic ones has been studied due to their low cost and to facilitate the subsequent stages of enzyme purification.…”

“…Since wheat flour and wheat bran both favored lipase production, the authors decided to experiment different residue combinations, and after some tests the ratio 3/2 (w/w) of wheat flour and wheat bran was chosen as the base medium for subsequent experiments. Coradi et al (2012) also perceived that the combination of two residues was the most suitable for lipase production by T. harzianum. The highest enzyme activity of 4.0 U/g was obtained after 96 h of fermentation, in a medium containing castor bean and sugarcane bagasse.…”

Fungal Lipase Production by Solid-State Fermentation

“…SSF has many advantages over SmF for microbial enzyme production namely high yield and productivities, lower operating costs, low-cost fermentation media (raw biomass, agro industrial residues), higher oxygen distribution, fewer operational troubles, simpler equipment and control system [10,20].…”

“…Compared to the cost of culture medium in SmF, SSF of agro-industrial solid wastes is inexpensive, and therefore this process is industrially and economically attractive [16,21]. Coradi et al [20] reported a cost of lipase production by Trichoderma viride in SSF ten times lower than in SmF. For instance, total capital investment needed for a production scale of 100 m 3 of lipase concentrate per year is 78% higher for SmF compared to SSF [17].…”

Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

“…The use of cheap agro--industrial waste as substrate in SSF is gaining more and more attention, but SF is still being used mostly as the easiest method in industrial bioprocessing due to its up and downstream process simplicity (Coradi et al, 2013;Hölker and Lenz, 2005). Several investigations have been done into SF using agro waste such as corn stover, bagasse, and husk (Nema et al, 2015;Oguntimein et al, 1992); de--seeded sunflower head (Patil and Dayanand, 2006); residual pant biomass from industrial processing of Artemisia annua (Chandra et al, 2010); and wheat straw and barn (Gomathi et al, 2012;Singh et al, 2009) as a carbon source.…”