Comparing the growth and the development of mouse pre-antral follicle in medium with PL (Platelet Layset) and with FBS

Pazoki, H; Eimani, Hussein; Farokhi, Farhad; Shahverdi, Abdolhossein; Yazdi, Reza Salman; Tahaei, Leila Sadat

doi:10.1016/j.mefs.2015.01.006

Cited by 13 publications

(15 citation statements)

References 23 publications

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“…Furthermore, the obtained results revealed no significant differences between PRP, L-GF equina treated mares, and control mares regarding the diameter of mature Graafian follicles. Similarly, platelet lysate induced a higher follicular survival with no effect follicle development during the in-vitro culture of isolated mouse secondary follicles [ 27 ]. In contrast, PRP improved ovarian function in cows with ovary hypofunction; this effect may be as a result of a reduction in follicular atresia or from revitalization of the dormant oocytes [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Dawod

Miró

Elbaz

et al. 2021

Animals

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This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium thickness increased significantly in the L-GFequina and PRP groups ahead of the non-treated group. Intrauterine L-GFequina or PRP administration shortened the estrus cycle length. A higher pregnancy rate was recorded in the L-GFequina and PRP treated mares. In conclusion, the intrauterine infusion of L-GFequina or PRP increased the endometrial thickness and pregnancy rate and could be used to improve fertility in Arabian purebred mares who experienced from repeat breeding.

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Section: Discussionmentioning

confidence: 99%

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Dawod

Miró

Elbaz

et al. 2021

Animals

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“…In addition, there is some evidence that growth factors, cytokines, IGF and EGF can affect maturation and follicle development [27,28]. In recent years, some studies have been conducted to improve the culture medium by adding different supplements like PL [22][23][24]. In this study, the maturation medium was enriched by different concentration of PL and maturation, fertilization and subsequent development were monitored.…”

Section: Discussionmentioning

confidence: 99%

“…Anna et al in 2007 and Lohmann et al in 2010 added PL to the cell culture media and the results showed that PL had good potential to support cell culturing and proliferation [22,23]. Pazoki et al in 2014 also used PL in pre-antral follicle culture medium for the first time and the result demonstrated that PL has an acceptable effect on oocyte growth [24]. The present study was carried out to consider whether PL has any positive effect on oocyte maturation at the GV stage.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies demonstrated that PL is replete with proteins and microelements along with growth factors such as fibroblast growth factor (FGF), TGFb, PDGF, IGF and EGF as well as attachment factors like fibrinogen and serotonin which are vital for cell culturing and folliculogenesis [20,21]. In recent years, some projects have been carried out using PL as a new additive supplement to improve media for cell culturing, and the results were impressive [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage

Pazoki

Eimani

Farokhi

et al. 2015

Reprod Medicine & Biology

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“…To obtain more training models, cells were cultured with different concentrations of FBS to establish cell viability gradients. FBS provides cells with basic nutrition, growth, and attachment factors (Pazoki et al, 2015). The Lack of FBS will lead to nonspecific adhesion which has no biological significance in studies in vitro, thus the microchannel of the microfluidic chip was coated by FN for specific adhesion.…”

Section: Cell Viability Heterogeneity Prediction Using Polynomial Regression Modelmentioning

confidence: 99%

Evaluating cell viability heterogeneity based on information fusion of multiple adhesion strengths

Wei

Zhang

et al. 2021

Biotech & Bioengineering

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Cell viability evaluation is significantly meaningful for cellular assays. Some cells with weak viability are easily killed in the detection of anticancer drugs, while others with strong viability survive and proliferate, ultimately leading to the treatment failure or the inaccuracy of biological assays. Accurately evaluating cell viability heterogeneity still remains difficult. This article proposed a multiphysical property information fusion method for evaluating cell viability heterogeneity based on polynomial regression in a single-channel integrated microfluidic chip. In this method, adhesion strengths τ N , that are defined as the magnitude of shear stress needed to detach (100 − N) % of cell population, were extracted as the independent variables of polynomial regression model by calculating the nonlinear fitting of the impedance-response curves for shear stress (cell detachment assay). Besides, by calculating the nonlinear fitting of the drug dose-response curves for cancer cells (IC 50 assay), the half-maximal inhibitory concentration (IC 50 ) was extracted as the dependent variables of polynomial regression model. The results show that the mean relative error of our fusion method averagely reduces by 6.04% and 62.79% compared with the multiple linear regression method and the cell counting method. Moreover, a simplified theoretical model used to describe the quantitative relationship between cell viability and its adhesion strengths was built to provide a theoretical basis for our fusion method.

show abstract

Comparing the growth and the development of mouse pre-antral follicle in medium with PL (Platelet Layset) and with FBS

Cited by 13 publications

References 23 publications

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage

Evaluating cell viability heterogeneity based on information fusion of multiple adhesion strengths

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