Comparison between blood hemoglobin concentration determined by point-of-care device and complete blood count in adult patients with dengue

Wisanuvej, Kantasit; Boonyawat, Kochawan; Savetamornkul, Chutchaiwat; Virapongsiri, Sirapong; Krongvorakul, Jatupon; Sungkanuparph, Somnuek; Phuphuakrat, Angsana

doi:10.1371/journal.pntd.0009692

Cited by 5 publications

(5 citation statements)

References 20 publications

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“… 11 Thrombocytosis is a condition in which a high platelet count (>450,000/μL) causes inappropriate blood coagulation. 12 In this study, 33.33% of NS1- and dengue IgM-reactive patients had a platelet count of less than 100,000/μL, and the rest 66.66% had a platelet count of more than 100,000/μL. The mean platelet count in reactive patients was found to be 138,033/μL in male patients and 143,315.8/μL in female patients.…”

Section: Discussionmentioning

confidence: 49%

“…An inadequate platelet count can lead to two different conditions: thrombocytopenia: a low platelet count (<150,000/μL) can cause excessive bleeding and bruising . Thrombocytosis is a condition in which a high platelet count (>450,000/μL) causes inappropriate blood coagulation . In this study, 33.33% of NS1- and dengue IgM-reactive patients had a platelet count of less than 100,000/μL, and the rest 66.66% had a platelet count of more than 100,000/μL.…”

Section: Discussionmentioning

confidence: 62%

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Study of Hepatic Dysfunction Associated with Dengue Epidemiology in a Tertiary Care Hospital, Kolkata

et al. 2023

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Dengue is a common arthropod-borne life-threatening febrile illness. This disease affects liver functions with an imbalance of liver enzymes followed by other clinical manifestations. The dengue serotypes can cause asymptomatic infection to more severe versions of hemorrhagic fever and dengue shock syndrome in West Bengal and around the globe. The main aim of this study is to establish how different liver enzymes act in identifying markers for dengue prognosis for the early detection of severe dengue fever (DF). The diagnosis of dengue patients was confirmed by enzyme-linked immunosorbent assay, and associated clinical parameters [aspartate transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase, total bilirubin, total albumin, total protein, packed cell volume, and platelet count] were analyzed. Furthermore, the viral load estimation was also carried out by RT PCR analysis. The majority of these patients had elevated AST and ALT levels; ALT levels were higher than AST levels, which were partially observed in all non-structural protein 1 antigen- and dengue immunoglobulin M antibody-reactive patients. Almost 25% of patients had very low platelet count or thrombocytopenia. Furthermore, the viral load shows a significant association with all the clinical parameters with a p-value of <0.0001. All these liver enzymes are significantly correlated with an increased level of T.BIL, ALT, and AST. This study depicts that the intensity of hepatic involvement may play a critical role in the morbidity and mortality of DF patients. As a result, all of these liver parameters can be useful early markers for determining the severity of the disease, allowing for early detection of high-risk cases.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 62%

Study of Hepatic Dysfunction Associated with Dengue Epidemiology in a Tertiary Care Hospital, Kolkata

et al. 2023

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“…The hemoconcentration phenomenon is present in many clinical scenarios such as induced hemorrhage, 13 exercise, 14 and heat stress 15 among many others. However, in the specific context of hemodialysis, there is lack of a validated gold standard or substantial consensus regarding the proper strategy to deal with hemoconcentration, leading to the indistinct use of outdated correction factors in modern studies.…”

Section: Discussionmentioning

confidence: 99%

An extended kinetic model-based correction factor equation to account hemodialysis post-treatment hemoconcentration

2023

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The hemoconcentration effect for middle weight solutes in hemodialysis is corrected by oversimplified methods based on hematocrit changes or distribution volume variations. Here we implemented a variable volume dual pool kinetic model targeted at obtaining a precise correction factor equation for extracellularly distributed solutes based on relevant kinetic parameters such as the ultrafiltration to dry weight ratio UF/DW, the dialyzer clearance, Kd, the intercompartment mass-transfer coefficient, Kc, and the central compartment to extracellular volume ratio, α. More than 300,000 solutions of the model were computed, performing a sweep among physiological values of the proposed kinetic parameters, resulting in a linear regression denoted by the expression fcorr = 1.0707 − 5.2246 (UF/DW) − 0.0005 Kd − 0.0004 Kc − 0.0007 α, with an excellent coefficient of determination R2 = 0.983. The presented fcorr provides a substantial extension of the currently implemented methods to estimate the hemoconcentration factor for middle and high molecular weight extracellular distributed solutes in hemodialysis.

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“…The alterations in platelet count and HCT values are primary manifestations in dengue patients and are associated with the severity of the disease 29 . The HCT elevation is a feature associated with systemic capillary leak and is also considered an essential diagnostic parameter for the determination of plasma leakage prompted by cytokine‐mediated alterations in vascular permeability before the onset of severe DHF/DSS 30,31 . Several pieces of evidence have reported that the decreased platelet count, raised HCT, and altered coagulation profile significantly influenced the dengue severity.…”

Section: Discussionmentioning

confidence: 99%

“…29 The HCT elevation is a feature associated with systemic capillary leak and is also considered an essential diagnostic parameter for the determination of plasma leakage prompted by cytokine-mediated alterations in vascular permeability before the onset of severe DHF/DSS. 30,31 Several pieces of evidence have reported that the decreased platelet count, raised HCT, and altered coagulation profile significantly influenced the dengue severity. In an experimental study involving 105 dengue-affected pediatric population, it was reported that DHF/DSS condition was associated with a severe decline in platelet count (<20,000/mm 3 ) in comparison to DF subjects who had mild to moderate thrombocytopenia.…”

Section: Discussionmentioning

confidence: 99%

Elevated serum PAR‐1 levels as an emerging biomarker of inflammation to predict the dengue infection severity

Sibia

Sood

Subedi

et al. 2022

Journal of Medical Virology

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The present study was designed to check the serum levels of protease‐activated receptor (PAR‐1) in patients during different phases of dengue severity. Moreover, a correlation between serum PAR‐1 levels and hematological parameters, inflammatory cytokine levels, and liver functional changes was also determined. Based on the World Health Organization criteria, the study population was divided into: nonsevere dengue fever (DF; n = 30), severe dengue hemorrhagic fever (DHF; n = 19), and severe dengue shock syndrome (DSS; n = 11). The platelet count (PLT) and hematocrit (HCT) were analyzed using an automated hematology analyzer and liver function enzymes aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphate (ALP), bilirubin were checked by auto‐analyzer using diagnostic kits. Moreover, the levels of inflammatory mediators C‐reactive protein (CRP), tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), interleukin‐17 (IL‐17), and PAR‐1 were determined using respective ELISA kits. The HCT levels were elevated and platelet count decreased significantly during dengue complications (DHF and DSS) compared to the DF patients, while the levels of liver functional biomarkers AST, ALT, ALP, and bilirubin remained elevated in DHF and DSS groups than in the corresponding DF group. Similarly, the inflammatory cytokine levels of CRP, TNF‐α, IL‐6, and IL‐17 in DHF and DSS subjects were markedly increased when observed against DF subjects. Notably, the PAR‐1 levels were significantly elevated in DHF and DSS groups than in the DF group and positively correlated with changes in HCT levels, inflammatory biomarkers, and liver enzymes. Our findings conclude that PAR‐1 levels persistently increased with the severity of the dengue infection and are strongly associated with various clinical manifestations. Thus, PAR‐1 levels can be used as a diagnostic marker for assessing dengue severity.

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Comparison between blood hemoglobin concentration determined by point-of-care device and complete blood count in adult patients with dengue

Cited by 5 publications

References 20 publications

Study of Hepatic Dysfunction Associated with Dengue Epidemiology in a Tertiary Care Hospital, Kolkata

Study of Hepatic Dysfunction Associated with Dengue Epidemiology in a Tertiary Care Hospital, Kolkata

An extended kinetic model-based correction factor equation to account hemodialysis post-treatment hemoconcentration

Elevated serum PAR‐1 levels as an emerging biomarker of inflammation to predict the dengue infection severity

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