Comparison between two multicomponent drug delivery systems based on PEGylated-poly (l-lactide-co-glycolide) and superparamagnetic nanoparticles: Nanoparticulate versus nanocluster systems

Antibody Conjugated PLGA Nanocarriers and Superparmagnetic Nanoparticles for Targeted Delivery of Oxaliplatin to Cells from Colorectal Carcinoma

“…The negative zeta potential value indicates the coating of IO with OA, which confers them good colloidal stability and it is in agreement with our previous study [33]. XRD analysis confirmed the superparamagnetic structure of IO-OA nanoparticles, i.e., Fe 3 O 4 (Figure S1), where X-ray peaks were identified similarly to previous work [33]. The superparamagnetic properties of the prepared nanoparticles were analyzed by VSM with a coercivity value of 3.91 Oe and a saturation magnetization (Ms) of 0.43 emu/g.…”

“…According to the DLS measurements, IO-OA displayed a mean diameter of 20 ± 4 nm and zeta potential of −36 ± 4 mV (Table 1). The negative zeta potential value indicates the coating of IO with OA, which confers them good colloidal stability and it is in agreement with our previous study [33]. XRD analysis confirmed the superparamagnetic structure of IO-OA nanoparticles, i.e., Fe 3 O 4 (Figure S1), where X-ray peaks were identified similarly to previous work [33].…”

“…The chemical shifts identified at 1.59 ppm, 3.61-3.64 ppm, 4.50-4.90 ppm, and 5.13-5.30 ppm correspond to the -CH 3 of the lactide, -CH 2 -of the ethylene glycol, -CH 2 -of the glycolide, and -CH-of the lactide, respectively. FTIR analysis further confirmed the formation of the diblock copolymer (Figure 2B), where the absorption band at 2883 cm −1 ascribed to the C-H stretching vibration of the methylene group of PEG chains was noticed in the PLGA-PEG-NH 2 spectrum, confirming the reproducibility of the synthesis and in agreement with our reported work [33].…”

“…The poly(lactide-co-glycolide)-poly(ethylene glycol) bearing amino end group (PLGA-PEG-NH2) diblock copolymer was synthesized as a step towards PEGylation of the PLGA nanoparticles to enhance their particle stability, prolong their circulation time when administered in the body and reduce their intermolecular aggregation through inter-particle steric repulsion [45][46][47]. Following the procedure reported in our previous work [33], the activation of the PLGA matrix was achieved by carbodiimide chemistry, using DCC/NHS and the subsequent attachment of the PEG diamine (NH2-PEG-NH2). The conjugation of the PEG diamine to the PLGA backbone was revealed by 1…”

“…Polymeric nanocarriers can also make it possibile to deliver therapeutic agents with different properties, within a multicomponent single particle, to substantially enhance the treatment efficacy [32][33][34][35]. Functionalities, such as drug delivery, targeting, magnetic hyperthermia, imaging, and radiotherapeutics may be combined in such nanocarriers.…”