Comparison of [5,5,5‐2H3]leucine and [ring‐2H5]phenylalanine tracers for the measurement of human apolipoprotein B100 kinetics

Maugeais, Cyrille; Ouguerram, Khadija; Maugeais, P.; Catherine, Simoneau; Gardette, J.; Magot, T.; Krempf, Michel

doi:10.1002/jms.1190300313

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…Albumin was isolated by differential solubility in trichloroacetic acid-absolute alcohol (32,50). The sample was dried by vacuum distillation and hydrolyzed by adding 1 ml of 4 N HCl into the dried albumin (40). Phenylalanine from albumin was enzymatically converted to phenylethylamine by L-tyrosine decarboxylase (9).…”

Section: Methodsmentioning

confidence: 99%

Protein anabolic effects of insulin and IGF-I in the ovine fetus

Shen

Wisniowski

Ahmed

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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We determined the effect of insulin and/or recombinant human (rh)IGF-I infusion on ovine fetal phenylalanine kinetics, protein synthesis, and phenylalanine accretion. The chronically catheterized fetal lamb model was used at 130 days gestation. All studies were performed while fetal glucose and amino acid concentrations were held constant. Experimental infusates were 1) saline, 2) rhIGF-I plus a replacement dose of insulin (40 nmol), 3) insulin (890 mIU/h), and 4) IGF-I plus insulin (40 nmol IGF-I/h and 890 mIU insulin/h). Both hormones increased glucose and amino acid utilization, with insulin having a greater effect. The major effect on phenylalanine kinetics was a pronounced fall in phenylalanine hydroxylation, again with insulin having the greatest effect. Whole body protein breakdown was not significantly altered by either hormone; whole body protein synthesis was significantly increased during the combined infusion. Protein accretion was increased by both hormones, with the greatest increase during combined infusion. The fractional synthetic rate (FSR) of circulating albumin was increased by IGF-I but not by insulin. Both hormones significantly increased skeletal muscle FSR without a synergistic effect. The anabolic effects of insulin and IGF-I were more pronounced in these studies than in previous studies where amino acid concentrations were not maintained. The present data also suggest that insulin and IGF-I promote fetal growth through distinct, organ-specific mechanisms.

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Section: Methodsmentioning

confidence: 99%

Protein anabolic effects of insulin and IGF-I in the ovine fetus

Shen

Wisniowski

Ahmed

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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“…It must be also pointed out that gentle hydrolysis must be used with deuterated tracers because of the possibility of hydrogen exchange when too harsh conditions are applied (17). This is a critical step because all other proteins are also hydrolyzed and then can dilute the tracer.…”

Section: Leucine Measurement Of Tracer/tracee Ratio In Apo B100mentioning

confidence: 99%

Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects

Maugeais

Ouguerram²,

Krempf³

et al. 1998

CCLM

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Kinetic disturbances of lipoprotein metabolism are important to know for a better understanding of lipid diseases or effects of drugs. These kinetic aspects were previously studied with radioactive tracers. The ethical concerns related to these tracers can be now overcome at a reasonable cost with the new development of small bench top mass spectrometers and the increased production of stable isotope tracers. In this review, we will discuss some methodological aspects related to stable isotope tracers and the analysis of the data with non-compartmental or compartmental models.

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A new labeling approach using stable isotopes to study in vivo plasma cholesterol metabolism in humans

et al. 2002

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Comparison of [5,5,5‐²H₃]leucine and [ring‐²H₅]phenylalanine tracers for the measurement of human apolipoprotein B₁₀₀ kinetics

Abstract: Incorporation of amino acids labeled with stable isotopes in apoliproproteins is

Cited by 5 publications

References 10 publications

Protein anabolic effects of insulin and IGF-I in the ovine fetus

Protein anabolic effects of insulin and IGF-I in the ovine fetus

Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects

A new labeling approach using stable isotopes to study in vivo plasma cholesterol metabolism in humans

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