Comparison of a Fluorescence Polarization Immunoassay of Netilmicin in Plasma, Peritoneal Dialysate, and Urine with a High-Performance Liquid Chromatographic Method

Forest, Jean‐Marc; Mailhot, Claude; Cartier, P; Sirois, G

doi:10.1097/00007691-199702000-00013

Therapeutic Drug Monitoring

1997

DOI: 10.1097/00007691-199702000-00013

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Comparison of a Fluorescence Polarization Immunoassay of Netilmicin in Plasma, Peritoneal Dialysate, and Urine with a High-Performance Liquid Chromatographic Method

Jean‐Marc Forest¹,

Claude Mailhot²,

P Cartier³

et al.

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Cited by 3 publications

References 24 publications

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Adsorptive stripping voltammetric determination of netilmicin in the presence of formaldehyde

Sun

et al. 2006

Anal Bioanal Chem

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A linear sweep adsorptive stripping voltammetric method for the determination of netilmicin in the presence of formaldehyde has been proposed for the first time. In the presence of 3.0 x 10(-3) g ml(-1) formaldehyde, netilmicin exhibits a sensitive cathodic peak at -1.30 V (vs. the saturated calomel electrode, SCE) in a medium of Britton-Robinson buffer (pH 8.7) with a scan rate of 100 mV s(-1) after a preconcentration period of 120 s at -1.10 V (vs. SCE). The peak current showed a linear dependence on the netilmicin concentration over the range 4.2 x 10(-9)-1.0 x 10(-7) g ml(-1). The achieved limits of detection and quantitation were 1.0 x 10(-10) and 3.3 x 10(-10) g ml(-1) netilmicin, respectively. It was deduced from the experiments that the amine-aldehyde condensation product formed between netilmicin and formaldehyde is mainly responsible for the appearance of the peak. The electrochemical behavior of netilmicin in the presence of formaldehyde has been studied. The method was applied to the direct determination of netilmicin in injectable formulations and spiked human urine and serum samples.

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Adsorptive stripping voltammetric determination of netilmicin in the presence of formaldehyde

Sun

et al. 2006

Anal Bioanal Chem

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Current methodologies for the analysis of aminoglycosides

Stead¹

2000

Journal of Chromatography B: Biomedical Sciences and Applicatio

205

142

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Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

1998

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Comparison of a Fluorescence Polarization Immunoassay of Netilmicin in Plasma, Peritoneal Dialysate, and Urine with a High-Performance Liquid Chromatographic Method

Cited by 3 publications

References 24 publications

Adsorptive stripping voltammetric determination of netilmicin in the presence of formaldehyde

Adsorptive stripping voltammetric determination of netilmicin in the presence of formaldehyde

Current methodologies for the analysis of aminoglycosides

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

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