Comparison of antimicrobial susceptibility patterns among coagulase-negative staphylococci

Price, Stuart; Flournoy, D. J.

doi:10.1128/aac.21.3.436

Cited by 38 publications

(21 citation statements)

References 11 publications

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“…The MICs of penicillin G for S. saprophyticus strains in this report are similar to those noted by previous investigators (9,12) and indicate intermediate susceptibility to this agent (14). Moreover, the MIC was independent of the ability of strains to produce ,3-lactamase.…”

Section: Resultssupporting

confidence: 80%

Staphylococcus saprophyticus beta-lactamase production and disk diffusion susceptibility testing for three beta-lactam antimicrobial agents

Latham¹,

Zeleznik²,

Minshew³

et al. 1984

Antimicrob Agents Chemother

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,B-Lactamase production and MIC determinations for penicillin, methicillin, and cephalothin were assessed for 67 strains of Staphylococcus saprophyticus and correlated with results of disk diffusion susceptibility testing. Fifty-five (82%) of the 67 strains produced 14-lactamase, and 40 (77%) of these ,B-lactamase-producing strains were susceptible (zone size, >29 mm) by disk diffusion techniques. Although the range of zone sizes for ,-lactamase producers was broad (26 to 36 mm), all 38 strains with a zone size of <31 mm by disk diffusion testing were 3-lactamase producers compared with 17 (59%) of 29 with larger zone sizes (P = 0.0000008). The median penicillin MIC for 12 S. saprophyticus strains was 0.25 ,g/ml and was not related to (-lactamase production. Although the methicillin MICs for 15 strains were in the susceptible range (4.0 ,Ig/ml), interpretation of disk diffusion testing for oxacillin varied greatly among laboratories using identically prepared media and standardized techniques. Criteria presently used to define susceptibility of Staphylococcus aureus to penicillin and oxacillin by disk diffusion are inappropriate for S. saprophyticus. The clinical significance of the l-lactamase produced by these strains needs further evaluation.Staphylococcus saprophyticus, a coagulase-negative staphylococcus, is a common cause of acute urinary infections in young women (3,6,15). Unlike most other coagulase-negative staphylococci, S. saprophyticus strains are reported to be uniformly susceptible to most antibiotics active against gram-positive organisms (3,9,15). Yet, in a recent study of urinary infections with this organism among college women (6), we found that the majority of strains were not susceptible to penicillin and methicillin by disk diffusion (DD) techniques, but were susceptible to cephalothin. To explain this observation, we assessed the prevalence of P-lactamase production among strains of S. saprophyticus, determined the MICs of penicillin, methicillin, and cephalothin for these strains, and correlated these results with susceptibility testing by DD. MATERIALS AND METHODS Sixty-seven S. saprophyticus strains isolated from ambulatory patients with acute urinary infections were evaluated.

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Section: Resultssupporting

confidence: 80%

Staphylococcus saprophyticus beta-lactamase production and disk diffusion susceptibility testing for three beta-lactam antimicrobial agents

Latham¹,

Zeleznik²,

Minshew³

et al. 1984

Antimicrob Agents Chemother

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“…Several investigators have reported that strains of Staph. haemolyticus are significantly more resistant to antimicrobial agents than other staphylococcal species (Price & Flournoy, 1982;Gill, Selepak & Williams, 1983;Hamilton-Miller & Illiffe, 1985;Davies et al 1986;Del Bene et al 1986). In the present study, strains of Staph.…”

Section: Discussionmentioning

confidence: 99%

Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

Deighton¹,

Franklin

Spicer

et al. 1988

Epidemiol. Infect.

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SUMMARYTwo hundred and seventy-five consecutive clinical isolates of coagulasenegative staphylococci, including strains associated with disease, contaminants and skin colonizers, were speciated, tested for slime production and for their sensitivity to a range of antibiotics. Staphylococcus epidermidis was the most commonly identified species, comprising 63 % of all isolates. Slime production was detected in half the strains of Staph. epidermidis, Staph. haemolyticus and Staph. saprophyticus but was rare in other species. Most Staph. haemolyticus strains and approximately half of the Staph. epidermidis strains were resistant to five or more antibiotics. A significant association was found between slime production and multiple antibiotic resistance. For catheter-associated strains, clinical relevance was predictable by species i.e. Staph. epidermidis. Multi-resistant slime-positive Staph. haemolyticus strains, although infrequently associated with disease, were common skin colonizers, presumably acquired from the hospital environment. We describe a practical and inexpensive scheme for the speciation of human coagulasenegative staphylococcal isolates.

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“…Among coagulase-negative staphylococci, tetracycline resistance, like other antibiotic resistances, is more common in S. epidermidis and S. haemolyticus than in the other species (18,19). However, tet(K) was detected in all the Tcr Mcs strains belonging to the various species studied ( (24) but also in 30% of methicillin-susceptible S. aureus strains and 5% of coagulasenegative Staphylococcus strains ( Table 1).…”

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confidence: 97%

Gene heterogeneity for tetracycline resistance in Staphylococcus spp

Bismuth

Zilhão

Sakamoto

et al. 1990

Antimicrob Agents Chemother

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Nucleotide sequences related to four tet genes were studied by hybridization in 183 clinical Staphylococcus isolates. tet(K) predominated in strains resistant only to tetracycline, while tet(M) was responsible for combined tetracycline and minocycline resistance. In strains harboring both genes, they contributed additively. tet(L) was detected in only five strains, and no hybridization was observed with tet(O).

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Comparison of antimicrobial susceptibility patterns among coagulase-negative staphylococci

Cited by 38 publications

References 11 publications

Staphylococcus saprophyticus beta-lactamase production and disk diffusion susceptibility testing for three beta-lactam antimicrobial agents

Staphylococcus saprophyticus beta-lactamase production and disk diffusion susceptibility testing for three beta-lactam antimicrobial agents

Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

Gene heterogeneity for tetracycline resistance in Staphylococcus spp

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