Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin

Abstract

Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max. Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed by 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay. Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in aging process. Results: ABTS-reducing activity assay showed that both compounds had great ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had low value of IC 50 ABTS-reducing activity around 14.39 µg/ml better than genistein with IC 50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC 50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin had higher antioxidant and anti-collagenase activity compared to Genistein.

Antioxidant Activities of Genistein: A Review

“…The formation of ROS can cause oxidative damage and tissue damage in the skin and cause loss of elasticity in the skin [18]. Genistein reported having acted as an antioxidant, which had a reduction activity value of IC 50, using the 2,2'-azinobis-3-ethylbenzo-thiazoline-6sulfonic acid (ABTS) method, which was around 43.17 µg/ mL [17].…”

Phenolic Compounds from Leaves and Flowers of Hibiscus roseus: Potential Skin Cosmetic Applications of an Under-Investigated Species

“…Therefore, these compounds might be responsible for the observed anti-collagenase activity. It is relevant to notice that different classes of phenolics, which are also present in our plant extracts, have already shown anti-aging activity via the inhibition of collagen degradation and contributing to skin humidification [ 78 ]. For example, ferulic acid and its derivatives have been proven to moisturize the skin and stimulate the synthesis of collagen fibers, being used in cosmetics such as anti-wrinkle creams [ 6 ].…”

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

“…Antimicrobial and antiviral (showed inhibition against Herpes simplex virus type 1 (HSV-1) (Musarra-Pizzo et al, 2019) Anti-oxidant, antitumor, antibacterial, antifungal, antidiabetic, anti-inflammatory, antiproliferative and antitumor (Cosarcã et al, 2019) Antioxidant, UV protective, anti-allergenic, anti-inflammatory, anti-microbial, anti-viral and anti-cancer (Bae et al, 2020) Antiviral (showed inhibition against pandemic influenza A (H1N1) (You et al, 2018) Epicatechin Antiviral (showed inhibitory effect against Mayaro (MAYV) virus and other alphavirus viruses (Ferreira et al, 2018) Antinociceptive and anti-inflammatory (Dias et al, 2018) Antibacterial (Ezhil and Lakshmi, 2017) Antidiabetic (Ibrahim et al, 2018) Anti-infective (Song, 2017) Anti-oxidant, antimicrobial, anti-inflammatory, antitumor, antidiabetic, anticancer and cardioprotective (Prakash et al, 2019) Antioxidant and anticollagenase (showed a high inhibitory power against the collagenase enzyme, responsible for the degradation of matrix collagen and the photo-aging process. (Geeta et al, 2019) UV protection (Prasanth et al, 2020)…”