Comparison of Aspiration, Touch and Scrape Preparations Simultaneously Obtained from Surgically Excised Specimens

Blumenfeld, Walter; Hashmi, Nighat; Sagerman, Paul

doi:10.1159/000332177

Cited by 16 publications

(14 citation statements)

References 3 publications

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“…Logically, scrape preparatives would be expected to contain more cells and therefore to be more appropriate than touch preparatives, as actually indicated by our results. This agrees with literature data showing that fresh tissue scrape preparatives are more efficient than simple touch preparatives when evaluated by cytological methods for preoperative diagnosis (10,23). The amount of DNA which can be extracted must therefore be larger in this type of material.…”

Section: Discussionsupporting

confidence: 91%

“…As determined here, these cytologic methods are of low cost, easy to set up and execute, and can be utilized on a large-scale basis (16). These advantages are also taken into account when cells are to be obtained for cytologic analysis (10,36) or even for fluorescent in situ hybridization (FISH).…”

Section: Discussionmentioning

confidence: 99%

“…Ethanol at 14% concentration, i.e., a concentration much lower than the one used here, has been used in studies of DNA extraction from oral mucosa cells obtained by oral brushing or washing and the results obtained have shown that DNA is of sufficient quality and quantity for the determination of gene sequences of different sizes (16). Ethanol is also the fixative used for cytologic analysis (10,36) and even for FISH (37,38), showing potential for adequate preservation not only of the chemical characteristics of cell components, but also of their morphological aspect. Cells obtained in this manner can therefore be utilized for different types of analysis, a fact supporting the importance of the definition of a protocol such as the one used here.…”

Section: Discussionmentioning

confidence: 99%

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DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells

Ribeiro

Peres

Neto

2004

Braz J Med Biol Res

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The objective of the present study was to develop a simplified low cost method for the collection and fixation of pediatric autopsy cells and to determine the quantitative and qualitative adequacy of extracted DNA. Touch and scrape preparations of pediatric liver cells were obtained from 15 cadavers at autopsy and fixed in 95% ethanol or 3:1 methanol:acetic acid. Material prepared by each fixation procedure was submitted to DNA extraction with the Wizard ® genomic DNA purification kit for DNA quantification and five of the preparations were amplified by multiplex PCR (azoospermia factor genes). The amount of DNA extracted varied from 20 to 8,640 µg, with significant differences between fixation methods. Scrape preparation fixed in 95% ethanol provided larger amount of extracted DNA. However, the mean for all groups was higher than the quantity needed for PCR (50 ng) or Southern blot (500 ng). There were no qualitative differences among the different material and fixatives. The same results were also obtained for glass slides stored at room temperature for 6, 12, 18 and 24 months. We conclude that touch and scrape preparations fixed in 95% ethanol are a good source of DNA and present fewer limitations than cell culture, tissue paraffin embedding or freezing that require sterile material, culture medium, laboratory equipment and trained technicians. In addition, they are more practical and less labor intensive and can be obtained and stored for a long time at low cost.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells

Ribeiro

Peres

Neto

2004

Braz J Med Biol Res

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“…Comparison of aspiration, touch prepara- tions and scrape preparations yield similar results most of the time. 9 However, increased cellularity may be observed on a scrape preparation as compared to a touch preparation and FNA. 9 …”

Section: Acta Cytologicamentioning

confidence: 99%

Cytologic Characteristics of Endocrine Ductal Carcinoma in <i>Situ</i> of the Breast

Yin

Schinella²

2002

Acta Cytologica

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“…In addition, some of the metastatic foci, especially macrometastases, had a fibrous stroma, which apparently makes detachment of carcinoma cells more difficult. Blumenfeld et al 25 compared the cellularity of material obtained by aspiration, touch and scraping. Scrape preparations yielded the most cellular specimens.…”

mentioning

confidence: 99%

Imprint Cytology of Sentinel Lymph Nodes in Breast Cancer

Sauer

Engh²,

Holck³

et al. 2003

Acta Cytologica

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Comparison of Aspiration, Touch and Scrape Preparations Simultaneously Obtained from Surgically Excised Specimens

Cited by 16 publications

References 3 publications

DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells

DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells

Cytologic Characteristics of Endocrine Ductal Carcinoma in <i>Situ</i> of the Breast

Imprint Cytology of Sentinel Lymph Nodes in Breast Cancer

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