Comparison of Basal Neuropeptide Y and Corticotropin Releasing Factor Levels Between the High Ethanol Drinking C57BL/6J and Low Ethanol Drinking DBA/2J Inbred Mouse Strains

Hayes, Dayna M.; Knapp, Darin J.; Breese, George R.; Thiele, Todd E.

doi:10.1097/01.alc.0000164375.16838.f3

Cited by 47 publications

(29 citation statements)

References 95 publications

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“…4G, 4H), with no overt species differences observed. Consistent with reports from other rodents, cellular immunoreactivity in BnST and CeA was likely obscured by high background from fiber staining [Asan, Yilmazer-Hanke, Eliava, Hantsch, Lesch, and Schmitt, 2005;Cummings, Elde, Ells, and Lindall, 1983;Hayes, Knapp, Breese, and Thiele, 2005;Sawchenko and Swanson, 1985]. CRF-immunoreactive fiber staining was also identified within the septal pole of the nucleus accumbens in both prairie and meadow voles (data not shown).…”

Section: Crf Immunohistochemistrysupporting

confidence: 87%

Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

et al. 2006

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Brain receptor patterns for the corticotropin-releasing factor (CRF) receptors, CRF1 and CRF2, are dramatically different between monogamous and promiscuous vole species, and CRF physiologically regulates pair bonding behavior in the monogamous prairie vole. However, little is known whether species differences also exist in the neuroanatomical distribution of the endogenous ligands for the CRF1 and CRF2 receptors, such as CRF and urocortin-1 (Ucn1). We compared the expression of CRF and Ucn1 in four vole species, monogamous prairie and pine voles, and promiscuous meadow and montane voles, using in situ hybridization of CRF and Ucn1 mRNA. Our results reveal that CRF mRNA expression patterns in all four vole species appear highly conserved throughout the brain, including the olfactory bulb, nucleus accumbens, bed nucleus of the stria terminalis, medial preoptic area, central amygdala, hippocampus, posterior thalamus, and cerebellum. Similarly, Ucn1 mRNA primarily localized to the Edinger-Westphal nucleus in all four vole species. Immunocytochemistry in prairie and meadow voles confirmed localization of CRF and Ucn1 protein to these previously identified brain regions. These data demonstrate a striking dichotomy between the extraordinary species diversity of brain receptor patterns when compared to the highly conserved brain distributions of their respective ligands. Our findings generate novel hypotheses regarding the evolutionary mechanisms underlying the neural circuitry of species-typical social behaviors. KeywordsCorticotropin-releasing hormone; CRF1; CRF2; median raphe; nucleus accumbens; EdingerWestphal nucleus List of AbbreviationsCRF Corticotropin-releasing factor; Ucn1 Urocortin 1; OB olfactory bulb; NAcc nucleus accumbens; BnST bed nucleus of stria terminalis; MPOA medial preoptic area; PVN paraventricular nucleus of the hypothalamus; hippo hippocampus; CeA central nucleus of the amygdala; thal posterior thalamus; MR median raphe nucleus; CBg cerebellum granular layer; EW Edinger-Westphal nucleus

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Section: Crf Immunohistochemistrysupporting

confidence: 87%

Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

et al. 2006

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“…Central neuropeptide systems have been hypothesized to acts as "alarm systems" that are preferentially activated under conditions of high neuronal activity, e.g., high fear or chronic stress (Hokfelt et al 1987;Karlsson and Holmes 2006). Also of note in this context is the finding that the genetic background used here, C57BL/6, has low levels of endogenous NPY in brain regions rich in Y1R (Hayes et al 2005;Parker and Herzog 1999). Therefore, the release of endogenous NPY under mild test provocation may be particularly low in this genetic background, leading to low Y1R recruitment and negligible consequences of Y1R deletion.…”

Section: Discussionmentioning

confidence: 94%

The neuropeptide Y Y1 receptor subtype is necessary for the anxiolytic-like effects of neuropeptide Y, but not the antidepressant-like effects of fluoxetine, in mice

et al. 2007

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“…Fluorescence was observed using a transmitted light microscope Olympus BX51 with a Plan Universal semi-apochromat Fluorite objective, UPlanFL 40X/0.75 Ph2, eyepieces WH10X/FN22, for a total magnification of ϫ400 and a resolution of 0.45 m. With the use of Magna Fire software (Magna Fire v2.1c), flat-field digital pictures were taken on Optronics camera connected to the microscope. The software ImageJ was used to process the background and to assess the colocalized fluorescence (18,34).…”

Section: Methodsmentioning

confidence: 99%

Effect of length oscillations on airway smooth muscle reactivity and cross-bridge cycling

Al‐Jumaily

Mbikou

Redey

2012

American Journal of Physiology-Lung Cellular and Molecular Phys

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Excessive airway narrowing due to airway smooth muscle (ASM) hyperconstriction is a major symptom in many respiratory diseases. In vitro imposition of length oscillations similar to those produced by tidal breathing on contracted ASM have shown to reduce muscle active forces, which is usually attributed to unconfirmed disruption of actomyosin cross-bridges. This research focuses on an in vitro investigation of the effect of mechanical oscillations on ASM reactivity and actomyosin cross-bridges. A computerized organ bath system was used to test maximally precontracted bovine ASM subjected to length oscillations at frequencies in the range of 10-100 Hz superimposed on tidal breathing oscillation. Using an immunofluorescence technique, two specific antibodies against the phospho-serine19 myosin light chain and the α-smooth muscle actin were used to analyze the colocalization between these two filaments. Data were processed using the plug-in "colocalization threshold" of ImageJ 1.43m software. The results demonstrate that both tidal and superimposed length oscillations reduce the active force in contracted ASM for a relatively long term and that the latter enhances the force reduction of the former. This reduction was also found to be frequency and time dependent. Additionally colocalization analysis indicates that length oscillations cause the detachment of the actomyosin connections and that this condition is sustained even after the cessation of the length oscillations.

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Comparison of Basal Neuropeptide Y and Corticotropin Releasing Factor Levels Between the High Ethanol Drinking C57BL/6J and Low Ethanol Drinking DBA/2J Inbred Mouse Strains

Abstract: These data suggest that low NPY levels in the amygdala and/or the shell of the NAc, which are not compensated for by similar changes in CRF levels, may contribute to the high ethanol consumption characteristic of C57BL/6J mice.

Cited by 47 publications

References 95 publications

Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

The neuropeptide Y Y1 receptor subtype is necessary for the anxiolytic-like effects of neuropeptide Y, but not the antidepressant-like effects of fluoxetine, in mice

Effect of length oscillations on airway smooth muscle reactivity and cross-bridge cycling

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