1995
DOI: 10.1002/bip.360360305
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Comparison of base inclination in ribo‐AU and deoxyribo‐AT polymers

Abstract: The inclination angle between the base normal and the helix axis is measured for ribo-AU polymers by using flow linear dichroism (LD), and compared to measurements for deoxyribo-AT polymers under dehydrating conditions. The CD of the DNA polymers under the dehydrating conditions is not the same as the corresponding RNA polymers, which are presumed to be in the A form. However, the LD indicates that poly(dAdT)-poly(dAdT) can assume the A form in 80% 2,2,2-trifluoroethanol, although poly(dA)-poly(dT) retains B f… Show more

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Cited by 3 publications
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“…However, it is important to note that the presence of deoxyribonucleotides did not affect the spectroscopic signals either of both fluorescence and far-UV CD, because DNA is spectroscopically silent in fluorescence, and in the far-UV region of CD spectra between 210–240 nm, deoxyribonucleotides do not absorb [ 44 , 45 ]. It is also interesting to note that deoxyoligonucleotides in aqueous solution or intact DNA show a small or slightly positive ellipticity around 222 nm [ 45 , 46 , 47 , 48 , 49 ] where we have carried out the study of the CD biophysical properties of the protein (see Results section).…”
Section: Methodsmentioning
confidence: 99%
“…However, it is important to note that the presence of deoxyribonucleotides did not affect the spectroscopic signals either of both fluorescence and far-UV CD, because DNA is spectroscopically silent in fluorescence, and in the far-UV region of CD spectra between 210–240 nm, deoxyribonucleotides do not absorb [ 44 , 45 ]. It is also interesting to note that deoxyoligonucleotides in aqueous solution or intact DNA show a small or slightly positive ellipticity around 222 nm [ 45 , 46 , 47 , 48 , 49 ] where we have carried out the study of the CD biophysical properties of the protein (see Results section).…”
Section: Methodsmentioning
confidence: 99%