Comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts

Nicola, Gina Rosalinda De; Bagatta, Manuela; Pagnotta, Eleonora; Angelino, Donato; Gennari, Lorenzo; Ninfali, Paolino; Rollin, Patrick; Iori, Renato

doi:10.1016/j.foodchem.2013.02.102

Cited by 67 publications

(56 citation statements)

References 40 publications

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“…In this paper, we have focused on volatile phytochemicals in sprouts, which are present at significant levels in members of the Brassica family. There was also a report on comparison of bioactive phytochemical content and release of ITCs in selected Brassica sprouts [20] . Their overall protective effect has been generally attributed to the modulation of xenobiotic metabolilizing enzymes and enzymatic antioxidative defense system [21] .…”

Section: Resultsmentioning

confidence: 99%

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Chaudhary¹,

Choudhary²,

Sharma³

et al. 2016

IJPS

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Chaudhary, et al.: In vitro Evaluation of Brassica SproutsThe present study was conducted on sprouts (5 and 7 days) of turnip (Brassica rapa), cauliflower (Brassica oleracea) and mustard (Brassica juncea) for bioactivity. Their antioxidant potential was assessed by 2,2-diphenyl-1-picrylhydrazyl, superoxide anion radical scavenging assays at 0.125-2 mg/ml concentration. The extract of turnip, cauliflower and mustard (5 and 7 days) showed a potent antioxidant effect and significant cytotoxic effect at 100 µg/ml concentration of extract. The antiproliferative potential was also evaluated by applying cell cycle and intracellular reactive oxygen species generation assay at IC 50 value. Various phytochemicals and hydrolytic products of glucosinolates were observed in a different extract of turnip, cauliflower, and mustard sprouts. Flow cytometric analysis showed that all the extracts caused an increase in a G 0 population of the PC-3 cells in cell cycle analysis and an increase in intracellular reactive oxygen species generation as compared to untreated cells. Confocal imaging of the cells stained with 4',6-diamidino-2-phenylindole and 2,7-dichlorodihydrofluorescein diacetate showed DNA fragmentation and increase of fluorescence which supports apoptosis and intracellular generation of the reactive oxygen species as the possible cause of cell death. The reported activity was correlated with the presence of different organosulfur compounds identified by gas chromatography-mass spectroscopy. Key words: Brassica sprout, antioxidant assay, cell cycle, ROS, confocal microscopy, MTT assayIn recent years, health protection by natural products or plant derived foods has received a considerable attention [1] . Specific groups of vegetables are particularly rich in potentially protective phytochemicals, especially Brassica contains a high concentration of constituents with antioxidant properties (e.g. carotenoids, vitamin C and folates) as well as glucosinolates precursors of isothiocyanates (ITCs) and indoles that modulate the activity of xenobiotic biotransformation [2] . A diet rich in fruits, cruciferous vegetables has been linked to reduce the risk of many chronic diseases, including cancer. The importance of this family for food crops has led to its selective breeding throughout the history. Cruciferous sprouts of broccoli, alfalfa, buckwheat and bean etc. have received considerable attention due to their rich content of health-promoting phytochemical constituents such as glucosinolates phenolic compounds and ascorbic acid related to cancer prevention as well as having antioxidant properties [3][4][5][6][7] .During sprouting generally, phytonutrient content increases as compared to seeds and consumption of these sprouts is the best way to gain all the health benefits [8,9] . Naturally occurring ITCs, found abundantly in cruciferous vegetables, inhibit tumorigenesis by inducing apoptosis and arresting cell cycle progression. In addition, induction of detoxification enzymes suggested the mechanism through which cruciferous v...

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Section: Resultsmentioning

confidence: 99%

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Chaudhary¹,

Choudhary²,

Sharma³

et al. 2016

IJPS

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“…SFEN's observed induction of G2/M phase cell cycle arrest and apoptosis can be in part explained by its ability to increase CDK1 phosphorylation at Thr 14 and Tyr 15 . SFEN is found in high concentrations in radish seeds and radish sprouts [31,32]. As radish sprouts are more common as a food ingredient, we analyzed the content of SFEN in radish sprouts at room temperature and at higher temperatures to mimic cooking conditions.…”

Section: Discussionmentioning

confidence: 99%

Sulforaphene suppresses growth of colon cancer‐derived tumors via induction of glutathione depletion and microtubule depolymerization

Byun

Shin

Park

et al. 2016

Molecular Nutrition Food Res

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“…This organic compound in its intact form has low biological activity whereas its hydrolytic products formed by the action of myrosinase enzyme exert high biological activities (Arora et al 2014a(Arora et al , b, 2016aBongoni et al 2014). Among the variety of hydrolytic products formed, isothiocyanates (ITCs) emerges with unsurpassed activities (De Nicola et al 2013;Arora et al 2014c). They act as an external and important factor in maintaining cellular homeostasis in oxidative stress conditions (Ghasemzadeh and Ghasemzadeh 2011;Cabello-Hurtado et al 2012).…”

Section: Introductionmentioning

confidence: 99%

3-Butenyl isothiocyanate: a hydrolytic product of glucosinolate as a potential cytotoxic agent against human cancer cell lines

et al. 2016

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The present study envisages the cytotoxic potential of 3-butenyl isothiocyanate isolated from Brassica juncea L. Czern var. Pusa Jaikisan against the human cancer cell lines viz. prostate, bone osteosarcoma, cervical, liver, neuroblastoma and breast cancer. As the compound was observed to be more effective against prostate cancer cell line, therefore, this cell line was further used to study the mechanism of cell death using neutral red assay, reactive oxygen species assay, mitochondrial membrane potential assay, microscopic and cell cycle analysis. The mechanistic analysis indicated that it induced the cell death of prostate cancer cells via apoptosis and hence made it an excellent choice as an effective anticancer compound.

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Comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts

Cited by 67 publications

References 40 publications

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Sulforaphene suppresses growth of colon cancer‐derived tumors via induction of glutathione depletion and microtubule depolymerization

3-Butenyl isothiocyanate: a hydrolytic product of glucosinolate as a potential cytotoxic agent against human cancer cell lines

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