Comparison of Biodistribution and Biocompatibility of Gelatin-Coated Copper Nanoparticles and Naked Copper Oxide Nanoparticles

Abstract

In this study, we investigated the biocompatibility of gelatin-coated copper nanoparticles. To estimate their cytotoxicity, the coated copper nanoparticles were exposed to osteoblastic cells. The cell proliferation remained above 80% even when the particles concentration increased. When uncoated copper oxide nanoparticles were exposed to the cells, the proliferation ratio rapidly decreased with the concentration reaching 20% under the same conditions. To determine their biodistribution, the nanoparticles were administered to mice through their tail veins. The particles were subsequently found in some organs using an energy-dispersed X-ray spectrometer and inductively coupled plasma-atomic emission spectroscopy. The polymer-coated nanoparticles were observed in the lung, liver and spleen. They were also detected in the urine at higher concentrations than the copper oxide nanoparticles. Thus, the polymer coating is expected to improve biocompatibility by virtue of the excellent cytocompatibility and acceleration of the excretion process.

Biocompatibility and Biodistribution of Several Nano-Sized Ceramics Particles

“…In our previous study, cupper nanoparticles coated by gelatin was synthesized and then exposed to MC3T3-E1 cells. Compared with CuO nanoparticles, the viability increased drastically [12]. Recently, we succeeded in time-lapse observation of cell dynamic behavior under nanoparticles exposure.…”

Copper–Ruthenium Composite as Perspective Material for Bioelectrodes: Laser-Assisted Synthesis, Biocompatibility Study, and an Impedance-Based Cellular Biosensor as Proof of Concept

“…Copper electrodes are often used to manufacture electrochemical sensing devices [48][49][50][51] (e.g., glucose sensors) and as a base for skin electrodes [52][53][54]. However, due to the high toxicity of copper and copper oxide [55][56][57], copper electrodes can not replace expensive noble metal electrodes in many important fields of bioelectronics such as impedance-based in vitro and in vivo cell and tissue studies (for example, using the ECIS method [6]), neuronal electrophysiological investigation with multi-electrode arrays [34], etc.…”

Effects of Surface Properties of Montmorillonite for their Cytocompatibility

“…Surface modification has been used as a strategy for improving cytocompatibility of materials, with several reports of cytocompatibility improvement because of surface modification. For example, Liu et al [10] reported that surface modification of carbon nanotube with carboxyl group improved its biocompatibility, while Abe et al [11] reported that biocompatibility of copper nanoparticles was improved using a gelatin coating. Yang et al [12] reported that poly(dimethylsiloxane) modified with polysaccharides showed higher cytocompatibility than the unmodified poly(dimethylsiloxane).…”