Comparison of bovine rumen liquor and bovine faeces as inoculum for an in vitro gas production technique for evaluating forages

Maurício, Rogério Martins; Owen, E.; Mould, F.L.; Givens, D.I.; Theodorou, Michael K.; Davies, David R.; Dhanoa, M. S.

doi:10.1016/s0377-8401(00)00234-0

Cited by 83 publications

(90 citation statements)

References 31 publications

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“…Difference in bacterial flora of faeces may explain discrepancies between studies. Lower activity of the microbial population present in faeces than in ruminal contents has been observed by Mauricio et al (2001), which could contribute to lower net production of EL by faecal than ruminal microbiota.…”

Section: Discussionmentioning

confidence: 91%

In vitrometabolism of flax lignans by ruminal and faecal microbiota of dairy cows

Côrtes

Gagnon

Benchaar

et al. 2008

Journal of Applied Microbiology

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Aims: To determine the in vitro conversion of plant lignans from two flax products (hull and seed) into the mammalian lignans, enterolactone and enterodiol, by bovine ruminal and faecal microbiota. Methods and Results: Flax seeds and hulls were incubated in vitro over a 96‐h time course with ruminal or faecal inoculum. Plant lignans in flax seeds and hulls averaged 9·2 and 32·0 nmol mg−1, respectively. The highest net production of enterodiol at 72 and 96 h of incubation was obtained with flax hulls incubated with faecal microbiota. There was no difference in net production of enterodiol between flax products within the first 24 h of incubation. In general, net production of enterolactone over the 96‐h time course was significantly higher for flax products incubated with ruminal than with faecal microbiota. Net production of enterolactone at 72 and 96 h of incubation was greater for flax hulls than flax seeds. Conclusions: Results of the present experiment suggest that, of the metabolites studied, the main mammalian lignan metabolite produced from flax hulls and seeds by ruminal microbiota is enterolactone while faecal microbiota leads mainly to the net production of enterodiol. Significance and Impact of the Study: This research will improve the understanding of the metabolic pathway of mammalian lignans in dairy cows, in order to enable targeted manipulation of their quantities in milk.

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Section: Discussionmentioning

confidence: 91%

In vitrometabolism of flax lignans by ruminal and faecal microbiota of dairy cows

Côrtes

Gagnon

Benchaar

et al. 2008

Journal of Applied Microbiology

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“…For the middle stage of in vitro fermentation, half-life, the time to reach half of asymptotic gas volume, can be a good choice and has been used in many in vitro studies (Mauricio et al, 2001;Calabrò et al, 2005;Jalilvand et al, 2008). For example, half-life ranged from 10 to 20 h for in situ degradation of 87 ruminant feeds (Ló pez et al, 1999) and from 8 to 50 h for in vitro gas production kinetics of 24 feeds (Mauricio et al, 2001;Calabrò et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…For example, half-life ranged from 10 to 20 h for in situ degradation of 87 ruminant feeds (Ló pez et al, 1999) and from 8 to 50 h for in vitro gas production kinetics of 24 feeds (Mauricio et al, 2001;Calabrò et al, 2005). For the late stage of in vitro fermentation, the time to produce 90% or 95% of total gas production can be a good choice for a rate parameter system (Murray et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Deriving fractional rate of degradation of logistic-exponential (LE) model to evaluate early in vitro fermentation

Wang

Sun

Tang

et al. 2013

Animal

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Water-soluble components of feedstuffs are mainly utilized during the early phase of microbial fermentation, which could be deemed an important determinant of gas production behavior in vitro. Many studies proposed that the fractional rate of degradation (FRD) estimated by fitting gas production curves to mathematical models might be used to characterize the early incubation for in vitro systems. In this study, the mathematical concept of FRD was developed on the basis of the Logistic-Exponential (LE) model, with initial gas volume being zero (LE 0 ). The FRD of the LE 0 model exhibits a continuous increase from initial (FRD 0 ) toward final asymptotic value (FRD F ) with longer incubation time. The relationships between the FRD and gas production at incubation times 2, 4, 6, 8, 12 and 24 h were compared for four models, in addition to LE 0 , Generalization of the Mitscherlich (GM), c th order Michaelis-Menten (MM) and Exponential with a discrete LAG (EXP LAG ). A total of 94 in vitro gas curves from four subsets with a wide range of feedstuffs from different laboratories and incubation periods were used for model testing. Results indicated that compared with the GM, MM and EXP LAG models, the FRD of LE 0 model consistently had stronger correlations with gas production across the four subsets, especially at incubation times 2, 4, 6, 8 and 12 h. Thus, the LE 0 model was deemed to provide a better representation of the early fermentation rates. Furthermore, the FRD 0 also exhibited strong correlations (P , 0.05) with gas production at early incubation times 2, 4, 6 and 8 h across all four subsets. In summary, the FRD of LE 0 model provides an alternative to quantify the rate of early stage incubation, and its initial value could be an important starting parameter of rate.Keywords: mathematical models, rate of gas production, logistic-exponential model, in vitro fermentation ImplicationsThe fractional rate of degradation (FRD) of LE 0 model generally had high correlation with gas production at 2, 4, 6 and 8 h across all four subsets tested, and thus provides a good alternative to reveal the rate of early stage in vitro fermentation. The FRD of LE 0 model would have an implication in quantifying the rate of fermentation and evaluating digestion efficiency of feedstuffs in the rumen, as ruminal retention time of feedstuffs is short. However, further studies are needed to testify whether a higher FRD leads to a higher nutritive value (e.g. volatile fatty acid profile, microbial protein synthesis and feedstuff digestibility) for ruminants.

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“…Cultures of ruminal or faecal microorganisms appear to result in similar fermentation processes (El-Meadaway et al, 1998). However, fewer microorganisms in faecal inocula may result in lower degradation capacity and decreased gas production (Cone et al, 2002;Vàradyovà et al, 2005), a longer lag phase and a slower rate of degradation at the outset (Mauricio et al, 2001). Furthermore, for poor-quality forages, there is only a weak relationship between gas production during incubations with inocula sourced from faeces and rumen fluid (El-Meadaway et al, 1998;Vàradyovà et al, 2005).…”

Section: Sampling Proceduresmentioning

confidence: 99%

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

136

120

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a b s t r a c tIn vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH 4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH 4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH 4 production.

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Comparison of bovine rumen liquor and bovine faeces as inoculum for an in vitro gas production technique for evaluating forages

Cited by 83 publications

References 31 publications

In vitrometabolism of flax lignans by ruminal and faecal microbiota of dairy cows

In vitrometabolism of flax lignans by ruminal and faecal microbiota of dairy cows

Deriving fractional rate of degradation of logistic-exponential (LE) model to evaluate early in vitro fermentation

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

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