Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

Abstract

Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.

Proline-based solution maintains cell viability and stemness of canine adipose-derived mesenchymal stem cells after hypothermic storage

“…In the present study, newly derived cAD-MSCs exhibited fibroblast-like shapes, culture-plate-adhesion capacity, and trilineage differentiation, which were similar to characteristics of human MSCs [ 14 , 15 , 18 ]. Besides, cAD-MSCs in our study had CD44 and CD90 expression of more than 95% but were deprived of CD34 and MHC class II expression, similar to a number of the previous reports [ 19 – 21 ].…”

Use of Magnetic Resonance Imaging to Assess the Regenerative Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in a Rabbit Cartilaginous Laryngeal Defect Model

“…The logarithmic growth phase cells were assessed by calculating the cell doubling time and counting using a hemocytometer. 23 All cells were suspended and then frozen in a solution containing 10% dimethyl sulfoxide (MP Biomedicals, Santa Ana, California) and 90% PBS. The cell suspension was aliquoted into sterile plastic cryo-vials, and the date, passage number, and freezing serial number were recorded.…”

Recent Trends in Multipotent Human Mesenchymal Stem/Stromal Cells: Learning from History and Advancing Clinical Applications

“…In addition, the authors also demonstrated that umbilical cord blood MSCs showed no adipogenic differentiation abilities compared to those from the bone marrow and adipose tissue [97]. Several studies including our own have demonstrated that indeed, adipose-derived MSCs can undergo multi-lineage differentiation under different conditions [9,[98][99][100].…”