Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods

Tanaka, Keiko; Iwasaki, Kengo; Feghali, Karine; Komaki, Motohiro; Ishikawa, Isao; Izumi, Yuichi

doi:10.1016/j.archoralbio.2010.10.013

Cited by 45 publications

(41 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While it is less expensive, the outgrowth method is more time consuming, and the proliferation of the cells it yields is slower than those obtained through enzymatic disaggregation. 24,44 In addition, outgrowth-isolated cells have lower stem-cell-marker expression than enzymatic disaggregated cells, and thus have lower multilineage differentiation potential. 23,24 One limitation of the present study is that different pulp tissues were used for the enzymatic disaggregation and outgrowth protocols.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 Previous studies have isolated multipotent stem cells from teeth using two methods: enzymatic disaggregation and outgrowth. [23][24][25][26] Deciduous teeth stem cells isolated by enzymatic disaggregation were found to be superior to those isolated using the outgrowth method, as assessed by measuring the expression of mesenchymal stem-cell markers and the osteogenic differentiation potential. 23 However, most of these studies have only evaluated the in vitro characteristics of the isolated stem cells; few studies have compared their in vivo characteristics when using different isolation methods.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

Jeon¹,

Song²,

Choi³

et al. 2014

Archives of Oral Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

Jeon¹,

Song²,

Choi³

et al. 2014

Archives of Oral Biology

View full text Add to dashboard Cite

“…Notably, the PDL fibroblasts used in this study are known to harbor stem cells (22)(23)(24)(25)(26). Indeed, stem cells derived from the periodontal membrane have been found to possess higher osteogenic potential than stem Fig.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

2017

View full text Add to dashboard Cite

Residual periodontal ligament (PDL)and cement mass on the roots of extracted teeth are factors that considerably affect tooth transplantation. Therefore, when normal extracted teeth are used for autologous transplantation, it is necessary to regenerate the PDL of the root surface. Here we describe a method to examine human PDL cell adhesion on sterilized root surfaces. Sample teeth were extracted during orthodontic treatment. PDL cells were obtained from healthy periodontal tissue explants from teeth extracted for orthodontic reasons. We developed a method for adhering PDL cells to sterile root surfaces using three-dimensional culture for 3 weeks. We evaluated the adhesion of human PDL cells to the sterilized root surfaces biochemically and histologically. The adherent PDL cells presented new projections on the sterile root surfaces. Therefore, PDL cells can adhere to sterile root surfaces.

show abstract

“…A high-voltage power supply was used at 17 kV. The distance between the tip-blunt capillary and the Human PDLCs were retrieved, using a method adopted from Tanaka et al, 24 from sound premolars extracted for orthodontic reasons from five individuals (16-18 years old, three males and two females). All procedures in the study in both in vitro cell culture and in vivo animal model followed the approved ethical guidelines set by the Ethics Committee of Nanjing Stomatological Hospital.…”

Section: Fabrication Of Electrospun Multiphasic Scaffoldmentioning

confidence: 99%

Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration

Chen

Liu

Miao

et al. 2016

IJN

View full text Add to dashboard Cite

Periodontitis is a major cause for tooth loss, which affects about 15% of the adult population. Cementum regeneration has been the crux of constructing the periodontal complex. Cementum protein 1 (CEMP1) is a cementum-specific protein that can induce cementogenic differentiation. In this study, poly(ethylene glycol) (PEG)-stabilized amorphous calcium phosphate (ACP) nanoparticles were prepared by wet-chemical method and then loaded with recombinant human CEMP1 (rhCEMP1) for controlled release. An electrospun multiphasic scaffold constituted of poly(ε-caprolactone) (PCL), type I collagen (COL), and rhCEMP1/ACP was fabricated. The effects of rhCEMP1/ACP/PCL/COL scaffold on the attachment proliferation, osteogenic, and cementogenic differentiations of human periodontal ligament cells, (PDLCs) were systematically investigated. A critical size defect rat model was introduced to evaluate the effect of tissue regeneration of the scaffolds in vivo. The results showed that PEG-stabilized ACP nanoparticles formed a core-shell structure with sustained release of rhCEMP1 for up to 4 weeks. rhCEMP1/ACP/PCL/COL scaffold could suppress PDLCs proliferation behavior and upregulate the expression of cementoblastic markers including CEMP1 and cementum attachment protein while downregulating osteoblastic markers including osteocalcin and osteopontin when it was cocultured with PDLCs in vitro for 7 days. Histology analysis of cementum after being implanted with the scaffold in rats for 8 weeks showed that there was cementum-like tissue formation but little bone formation. These results indicated the potential of using electrospun multiphasic scaffolds for controlled release of rhCEMP1 for promoting cementum regeneration in reconstruction of the periodontal complex.

show abstract

Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods

Cited by 45 publications

References 38 publications

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration

Contact Info

Product

Resources

About