Comparison of Collagen Cross-Linking and Amniotic Membrane Transplantation in an Experimental Alkali Burn Rabbit Model

Subaşı, Sevgi; Altıntaş, Özgül; Yardımoğlu, Melda; Yazır, Yusufhan; Karaman, Suleyman Korhan; Rençber, Selenay Furat; Kavram, Kubra

doi:10.1097/ico.0000000000001276

Cited by 15 publications

(9 citation statements)

References 33 publications

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“…fibronectin, EGF, retinoic acid). They have all been shown to promote re-epithelialisation of the ocular surface in animal models of alkali chemical burns [62,[75][76][77]. These therapies are still awaiting clinical trials in humans to determine their efficacy.…”

Section: Sutureless Amniotic Membranementioning

confidence: 99%

An update on chemical eye burns

Bizrah

Yusuf²,

Ahmad

2019

Eye

106

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Ocular chemical injuries vary in severity, with the more severe end of the spectrum having profound visual consequences and medicolegal implications. Grading of ocular injuries is critical for determining acute treatment and visual prognosis. Poor immediate management results in more challenging treatment of acute disease. Similarly, poorly controlled acute disease results in more treatment-resistant chronic ocular disease. Despite several decades of research and public health initiatives, simple and effective interventions such as wearing protective eyewear and immediate irrigation of eyes remain as key challenges. Education and prevention are therefore important public health messages. Hurdles in the acute management of disease include poor evidence-base for commonly used treatments (e.g. based on experimental animal studies), reduced treatment adherence rates and high clinic non-attendance rates. The evolution of treatment strategies, particularly limbal stem cell transplantation, has revolutionised the visual and cosmetic outcomes in chronic phases of disease. It is therefore increasingly important to consider tertiary referral for patients with limbal stem cell failure or vision-limiting corneal scarring.

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Section: Sutureless Amniotic Membranementioning

confidence: 99%

An update on chemical eye burns

Bizrah

Yusuf²,

Ahmad

2019

Eye

106

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“…Rabbits are exclusively used as keratectomy model ( Figure 2 ), where corneal ablation is performed by placing a NaOH soaked disk over the cornea ( Guo et al, 2011 ; Reza et al, 2011 ; Zeng et al, 2014 ; Subasi et al, 2017 ; Navas et al, 2018 ; Yamashita et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding corneal diseases, we identified 12 studies using PnD: five aiming to treat corneal infections, four evaluating corneal epithelialization in keratectomy models and three evaluating its effect in alkali burns. The majority ( n = 8) was treated with hAM as an over tissue, three with subconjunctival injections of hUC-MSC or hAMSC and one with an intra-cameral injection of hAMSC ( Heiligenhaus et al, 2005 ; Bauer et al, 2007 , 2009 ; Barequet et al, 2008 ; Guo et al, 2011 ; Reza et al, 2011 ; Kiilgaard et al, 2012 ; Subasi et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Insights and future directions for the application of perinatal derivatives in eye diseases: A critical review of preclinical and clinical studies

Norte-Muñoz

Botelho

Schoeberlein

et al. 2022

Front. Bioeng. Biotechnol.

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Perinatal derivatives (PnD) are gaining interest as a source for cell-based therapies. Since the eye is easily accessible to local administration, eye diseases may be excellent candidates to evaluate novel therapeutic approaches. With this work, we performed a systematic review of published preclinical and clinical studies addressing PnD in the treatment of ocular diseases. We have set two specific objectives: (i) to investigate the current level of standardization in applied technical procedures in preclinical studies and (ii) to assess clinical efficacy in clinical trials. Hereto, we selected studies that applied amniotic membrane (hAM) and mesenchymal stromal cells derived from amniotic membrane (hAMSC), placenta (hPMSC), umbilical cord (hUC-MSC) and Wharton’s Jelly (hUC-WJ-MSC), excluding those where cells were not transplanted individually, following a systematic PubMed search for preclinical studies and consultation of clinical studies on https://clinicaltrials.gov and https://www.clinicaltrialsregister.eu/. Our bibliographic search retrieved 26 pre-clinical studies and 27 clinical trials. There was a considerable overlap regarding targeted ocular structures. Another common feature is the marked tendency towards (i) locally administered treatments and (ii) the PnD type. In the cornea/ocular surface, hAM was preferred and usually applied directly covering the ocular surface. For neuroretinal disorders, intra-ocular injection of umbilical or placental-derived cells was preferred. In general, basic research reported favourable outcomes. However, due to lack of standardization between different studies, until now there is no clear consensus regarding the fate of administered PnD or their mode of action. This might be accountable for the low index of clinical translation. Regarding clinical trials, only a minority provided results and a considerable proportion is in “unknown status”. Nevertheless, from the limited clinical evidence available, hAM proved beneficial in the symptomatic relief of bullous keratopathy, treating dry eye disease and preventing glaucoma drainage device tube exposure. Regarding neuroretinal diseases, application of Wharton’s Jelly MSC seems to become a promising future approach. In conclusion, PnD-based therapies seem to be beneficial in the treatment of several ocular diseases. However, much is yet to be done both in the pre-clinical and in the clinical setting before they can be included in the daily ophthalmic practice.

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“…These findings were assessed as that usage of AMN + A or PRP would not provide efficient contribution to the regression of opacity. And when looked dissenting investigations, some researchers stated that the amniotic membrane transplantation is ineffective, even exacerbating, in reducing inflammation, opacity and neovascularization especially in the early phases of the corneal alkali burn lesion [33] . Our results might be interpreted as that combining the amniotic membrane with propolis might accelerate the reducing of the opacity but might not affect the final outcome.…”

Section: Discussionmentioning

confidence: 99%

Tavşanlarda Korneanın Subakut Alkali Yanıklarında Amniyotik Membran Transplantasyonu, Topikal Su Bazlı Propolis Ekstraktı, Kortikosteriod ve Antibiyotiğin Farklı Kombinasyonlarda Kullanımının Etkinliğinin Karşılaştırılması

Bozkan¹,

Belge²,

Sarıerler³

et al. 2019

Kafkas Univ Vet Fak Derg

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Investigation of the superiority of the usage of propolis extract, amniotic membrane transplantation, corticosteroid and antibiotic both separately and in different combinations was aimed. A total of 40 (20 male, 20 female) New Zealand breed rabbits weighing 2.5-3.0 kg were used in the study. The rabbits were divided into 5 groups on day 7 of alkali burn injury. Amniotic membrane transplantation was applied to 3 groups and each one of these 3 groups had different local medications as 1% water-based propolis extract (AMN+PRP), dexamethasone and tobramycin (AMN+AC) and only tobramycin (AMN+A). The other two groups, which were not performed amniotic membran transplantation, were either treated with propolis (PRP) or left as a control. The defect area results obtained from AMN+PRP group were lower than AMN+A group, and both AMN+PRP and control group at the day 9 and day 14, respectively (P≤0.05). In the measurements made after the second week, AMN+PRP group showed significantly better results in all weeks in terms of defect area (P≤0.05). Corneal thickness was found to be lower in all groups treated with amniotic membrane than in the other groups (P<0.05). This study aimed to find treatment effectiveness of combining the amniotic membrane with propolis, especially as an anti-inflammatory agent comparing to corticosteroids, and it was decided the outcomes can be improved on the further studies..

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Comparison of Collagen Cross-Linking and Amniotic Membrane Transplantation in an Experimental Alkali Burn Rabbit Model

Abstract: CXL treatment is an effective adjuvant treatment for promoting reepithelialization, reducing inflammation and neovascularization, and preventing ulceration in acute alkali burns. Providing AMT after suppressing inflammation may be a more effective treatment.

Cited by 15 publications

References 33 publications

An update on chemical eye burns

An update on chemical eye burns

Insights and future directions for the application of perinatal derivatives in eye diseases: A critical review of preclinical and clinical studies

Tavşanlarda Korneanın Subakut Alkali Yanıklarında Amniyotik Membran Transplantasyonu, Topikal Su Bazlı Propolis Ekstraktı, Kortikosteriod ve Antibiyotiğin Farklı Kombinasyonlarda Kullanımının Etkinliğinin Karşılaştırılması

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