Comparison of core needle biopsy and fine-needle aspiration of enlarged peripheral lymph nodes for antemortem diagnosis of enzootic bovine lymphosarcoma in cattle

Abstract

Results indicated that core needle biopsy and fine-needle aspiration can aid in the antemortem diagnosis of bovine enzootic lymphosarcoma. Results of fine-needle aspiration of enlarged peripheral lymph nodes were more specific and more predictive for a positive test result than were results of core needle biopsy.

Utility of Serum Thymidine Kinase Activity Measurements for Cases of Bovine Leukosis with Difficult Clinical Diagnoses

“…Clinical findings, including superficial lymph node swelling, lymphocytosis and detection of neoplastic lymphocytes in peripheral blood, are sufficient for suspicion of BL [10]. A definitive diagnosis is usually obtained by cytology of aspirates from tumors or tumorous nodes; however, the sensitivity and specificity of fine-needle aspiration (FNA) of enlarged peripheral lymph nodes are not always reliable [15]. Definitive diagnosis of BL is difficult in cattle without lymphadenopathy, even with evidence of lymphocytosis and atypical lymphocytes in the peripheral blood [12].…”

Ultrasonographic appearance of lymphomatous infiltration of the abomasum in cows with lymphoma

“…Subsequent testing confirmed the diagnosis in all patients. 6 However, false-negative results can occur with reactional lymphadenopathy. 9 Although the clinical signs of lymphoma may be obvious in typical patients examined with differing degrees of cachexia, polyadenomegaly, melena, congestive heart failure, and ataxia resulting from neurologic involvement, the diagnosis is more challenging when only internal organs are infiltrated by neoplastic tissue.…”

“…Although the incidence of gastrointestinal tumors is low in cattle, 8 lymphoma is the most common neoplastic disease in cattle in areas where BLV infection has not been eradicated. 6 Clinically, the challenge occurs when no specific clinical signs are noted on physical examination or when lymphadenomegaly or organomeg-aly is suspected on the basis of the results of transrectal examination, but the mass cannot be aspirated safely. The challenge for the clinician is the rapid establishment of an antemortem diagnosis while avoiding unnecessary treatments or expensive diagnostic procedures such as exploratory laparotomy.…”

“…Abomasal or duodenal obstruction was suspected on the basis of the abnormalities on the CBC and serum biochemical analysis. Additionally, percutaneous fine-needle (20-gauge) aspiration 6 of the abnormal pyloric wall revealed hemorrhagic content. 1 The abomasum was severely distended when compared with results of previous studies 2,4 in dairy cows, and its caudal margin was visible 20 cm caudal to the last rib.…”

Evaluation of Gene Expression in Peripheral Blood Cells as a Potential Biomarker for Enzootic Bovine Leukosis

“…Findings that lead to suspicions of EBL include lymphocytosis, enlargement of peripheral lymph nodes and the presence of neoplastic lymphocytes in peripheral blood [12]. In general, fine needle aspiration (FNA) cytology of primary neoplasms or neoplastic lymph nodes can lead to a definitive diagnosis of EBL, but the sensitivity and specificity of FNA are not confidence [30]. Furthermore, diagnosis can be difficult for EBL which lacks lymphocytosis and enlargement of lymph nodes [12].…”