Comparison of Different PCR Tests to Detect Bovine Lentivirus in Cell Culture and Experimentally and Naturally Infected Cattle

Evidence of bovine immunodeficiency virus (BIV) infection: Serological survey in Argentina

“…This is supported by our negative PCR analysis and previous results (Gonzalez et al, 2001;Lew et al, 2004). On the other hand, Suarez and Whettone (1997) demonstrated variability (from 0 to 10 out of 12) in the classification of infection status of samples from experimentally infected bovines using the PCR assay.…”

Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

“…There was a significant increase in the CD4/CD8 ratio in BIV-infected cows due to a significant decrease in CD8 counts. Previous studies have shown there are no significant changes in lymphocyte counts and CD4/CD8 ratios [3,7,9,28,29] and increased [9] CD8 counts. It is unclear why such differences occur, but immune dysfunction and/or some changes in leukocyte proportions might be present only temporarily after BIV infection [9] and concurrent BIV and BLV infection may play an important role.…”

Evaluation of alternative methods for the detection of bovine leukaemia virus in cattle

“…In the present study, however, the numbers ofserologically positive yet PCR-negative animals was disproportionally greater than the number of serologically negative, PCR-positive ones. Recent PCR results for a related virus (bovine immunodeficiency virus (BIV)) point to similar problems in detecting virus in field samples, as compared to cell culture or experimentally infected animals (Suarez and Whetstone, 1997). The unsatisfactory performance ofthe PCR compared to the serological tests suggests, however, that using blood samples for PCR assays is not appropriate for large scale application in an eradication campaign, but may in selected cases be ofuse as a confirmatory test where the AGID fails to give a positive result.…”