Comparison of different strains of the yeastYarrowia lipolytica for citric acid production from glucose hydrol

Wojtatowicz, M.; Rymowicz, Waldemar; Kautola, Helena

doi:10.1007/bf02921787

Cited by 60 publications

(56 citation statements)

References 14 publications

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“…2 and 3). To the best of our knowledge, the highest Y Cit/S value for glucose or similarly metabolized compounds was reported by Wojtatowicz et al [7], who found a Y Cit/S of 0.93 g/g in experiments with a strain of Y. lipolytica grown on hydrolyzed potato starch containing 15% glucose. In contrast, in most reports lower yields were obtained compared with the present study (e.g., 0.45-0.60 g/g) [6, 11, 12, 16-18, 23, 28, 29, 33, 47].…”

Section: D912mentioning

confidence: 70%

“…However, during the last few years numerous Yarrowia (Candida) lipolytica strains (natural or mutant) have been shown to be capable of producing citric acid in significant quantities during cultivation on various hydrophilic or hydrophobic substrates and fermentation configurations [6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Due to the easier handling of yeast compared to mold culture and the potential of growing yeasts on various carbon substrates, citric acid production has became one of the most important biotechnological applications of Y. lipolytica yeast [20].…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose

Papanikolaou

Chatzifragkou

Fakas

et al. 2009

Euro J Lipid Sci & Tech

150

101

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The biochemical behavior of wild-type or genetically modified (presenting decreased expression of intracellular acyl-CoA oxidases) Yarrowia lipolytica strains cultivated on commercial glucose was studied. Flask nitrogen-limited cultures were performed favoring the production of organic acids (and potentially the accumulation of lipid). Nitrogen depletion induced secretion of citric acid, while intracellular lipid was not produced in high quantities. Maximum total citric acid up to 49 g/L (yield 0.85 g/g glucose) was produced. In some of the wild-type strains, an increase of glucose in the medium also induced noticeable production of acetic acid. Increasing the amount of added glucose led to an increase in the total lipid quantity (%) produced, although in the stationary growth phase the concentration of lipid declined, indicating lipid degradation even for the genetically modified strains. Total lipid amount did not exceed the value of 14%, while neutral fractions increased with increase in glucose concentration. In all cases, the total microbial lipids and major lipid fractions were composed of C 16 and C 18 (principally unsaturated) fatty acids. Finally, in several of the strains cultured in media containing a low glucose concentration, unicellular morphology was observed, while at high glucose concentrations mycelia were predominant.

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Section: D912mentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose

Papanikolaou

Chatzifragkou

Fakas

et al. 2009

Euro J Lipid Sci & Tech

150

101

View full text Add to dashboard Cite

show abstract

“…Therefore, citric acid is considered to be the major bioproduct of this engineered strain. Previous research has reported citric acid production at a yield of 0.93 g/g of glucose hydrol from this yeast [38]. Another study reported a mass yield of 0.90 g citric acid from each gram of glycerol containing waste [34].…”

Section: Lipid Productionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Abghari

Chen

2017

Fermentation

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Increasing demand for plant oil for food, feed, and fuel production has led to food-fuel competition, higher plant lipid cost, and more need for agricultural land. On the other hand, the growing global production of biodiesel has increased the production of glycerol as a by-product. Efficient utilization of this by-product can reduce biodiesel production costs. We engineered Yarrowia lipolytica (Y. lipolytica) at various metabolic levels of lipid biosynthesis, degradation, and regulation for enhanced lipid and citric acid production. We used a one-step double gene knock-in and site-specific gene knock-out strategy. The resulting final strain combines the overexpression of homologous DGA1 and DGA2 in a POX-deleted background, and deletion of the SNF1 lipid regulator. This increased lipid and citric acid production in the strain under nitrogen-limiting conditions (C/N molar ratio of 60). The engineered strain constitutively accumulated lipid at a titer of more than 4.8 g/L with a lipid content of 53% of dry cell weight (DCW). The secreted citric acid reached a yield of 0.75 g/g (up to~45 g/L) from pure glycerol in 3 days of batch fermentation using a 1-L bioreactor. This yeast cell factory was capable of simultaneous lipid accumulation and citric acid secretion. It can be used in fed-batch or continuous bioprocessing for citric acid recovery from the supernatant, along with lipid extraction from the harvested biomass.

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“…To carry out fermentation processes, in addition to very expensive food raw materials, such as glucose [4][5][6][7] and plant oils [8][9][10], much cheaper substrates which are waste products of various industries, such as glycerol-containing waste of the biodiesel industry [9][10][11][12][13][14][15][16][17], glucose-containing wood hydrolysates [18,19], olive mill waste-water [20], and inulin [21] are used. Ethanol, a water-soluble individual compound which ensures the formation of a pure product and facilitates the isolation process, is also of great importance [22,23].…”

Section: Introductionmentioning

confidence: 99%

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

2018

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Abstract:The world market of citric acid (CA) is one of the largest and fastest growing markets in the biotechnological industry. Microbiological processes for CA production have usually used the mycelial fungi Aspergillus niger as a producer and molasses as a carbon source. In this paper, we propose methods for CA production from renewable carbon substrates (rapeseed oil, glucose, glycerol, ethanol, glycerol-containing waste of biodiesel industry and glucose-containing aspen waste) by the mutant strain Yarrowia lipolytica NG40/UV5. It was revealed that Y. lipolytica grew and synthesized CA using all tested raw materials. The obtained results are sufficient for industrial use of most of the raw materials studied for CA production. Using rapeseed oil, ethanol and raw glycerol (which is an important feedstock of biodiesel production), a high CA production (100-140 g L −1 ) was achieved.

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Comparison of different strains of the yeastYarrowia lipolytica for citric acid production from glucose hydrol

Cited by 60 publications

References 14 publications

Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose

Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

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