Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model

Tsumanuma, Yuka; Iwata, Takanori; Washio, Kaoru; Yoshida, Toshiyuki; Yamada, Azusa; Takagi, Ryo; Ohno, Takahiro; Lin, Konghua; Yamato, Masayuki; Ishikawa, Isao; Okano, Teruo; Izumi, Yuichi

doi:10.1016/j.biomaterials.2011.04.071

Cited by 273 publications

(250 citation statements)

References 36 publications

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“…Such cell sheet integration methods may provide a possible solution for the clinical repair of various damaged organs, including impaired myocardium. (29) performed cell sheet transplantation using three types of mesenchymal tissue-derived cells (periodontal ligament, alveolar periosteum and bone marrow) to compare differences between cell sources in a canine severe defect (one-wall intrabony defect) model. Three-layered cell sheets from each cell source, supported with woven polyglycolic acid, were transplanted autologously to the denuded root surface.…”

Section: Myocardial Tissue Engineeringmentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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Section: Myocardial Tissue Engineeringmentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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“…Current tissue-engineering strategies to recover lost tissue typically involve a combination of autologous cells and growth factors with various biomaterials for guided tissue regeneration (GTR) and guided bone regeneration (GBR), or human PDL cell sheets (14)(15)(16)(17)(18). However, the outcomes of GTR, GBR, and the use of cell sheets depend on the state of the recipient.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

2017

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Residual periodontal ligament (PDL)and cement mass on the roots of extracted teeth are factors that considerably affect tooth transplantation. Therefore, when normal extracted teeth are used for autologous transplantation, it is necessary to regenerate the PDL of the root surface. Here we describe a method to examine human PDL cell adhesion on sterilized root surfaces. Sample teeth were extracted during orthodontic treatment. PDL cells were obtained from healthy periodontal tissue explants from teeth extracted for orthodontic reasons. We developed a method for adhering PDL cells to sterile root surfaces using three-dimensional culture for 3 weeks. We evaluated the adhesion of human PDL cells to the sterilized root surfaces biochemically and histologically. The adherent PDL cells presented new projections on the sterile root surfaces. Therefore, PDL cells can adhere to sterile root surfaces.

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“…Common periodontal regenerative materials find limited applications when dealing with large bone defects such as a one-wall bone defect. The prognosis for this condition is quite poor owing to the difficulties faced in stabilizing the graft material until regeneration starts [5,13]. An earlier study on M. nemestrina and a human mandible showed the first evidence of improved bone regeneration when using chitosan as a scaffold [4,9].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

et al. 2018

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Methods:The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively. Conclusion:Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.

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Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model

Cited by 273 publications

References 36 publications

Application of the cell sheet technique in tissue engineering

Application of the cell sheet technique in tissue engineering

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

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