Comparison of Electrophoretic Protein Profiles from Sheep and Goat Parotid Saliva

Lamy, Elsa; Costa, Gustavo; Silva, Fernando Capela e; Potes, J.; Coelho, Ana Varela; Sales-Baptista, Elvira

doi:10.1007/s10886-008-9442-2

Cited by 38 publications

(34 citation statements)

References 56 publications

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“…Distel and Provenza (1991) did not find TBSPs in the saliva sampled from the oral cavity of goats and Lamy et al (2008) did not find TBSPs in sheep and goat parotid saliva. Austin et al (1989) did not identify TBSPs in cattle and the tannin-binding capacity of salivary proteins for tannins did not differ between cows fed high-or low-tannin diets (Makkar and Becker 1998).…”

Section: Introductionmentioning

confidence: 85%

No Major Role for Binding by Salivary Proteins as a Defense Against Dietary Tannins in Mediterranean Goats

et al. 2010

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We investigated whether Mediterranean goats use salivary tannin-binding proteins to cope with tannin-rich forages by determining the affinity of salivary or parotid gland proteins for tannic acid or quebracho tannin. Mixed saliva, sampled from the oral cavity, or parotid gland contents were compared to the intermediate affinity protein bovine serum albumin with a competitive binding assay. Goats that consume tannin-rich browse (Damascus) and goats that tend to avoid tannins (Mamber) were sequentially fed high (Pistacia lentiscus L.), low (vetch hay), or zero (wheat hay) tannin forages. Affinity of salivary proteins for tannins did not differ between goat breeds and did not respond to presence or absence of tannins in the diet. Proteins in mixed saliva had slightly higher affinity for tannins than those in parotid saliva, but neither source contained proteins with higher affinity for tannins than bovine serum albumin. Similarly, 3 months of browsing in a tannin-rich environment had little effect on the affinity of salivary proteins for tannin in adult goats of either breed. We sampled mixed saliva from young kids before they consumed forage and after 3 months of foraging in a tannin-rich environment. Before foraging, the saliva of Mamber kids had higher affinity for tannic acid (but not quebracho tannin) than the saliva of Damascus kids, but there was no difference after 3 months of exposure to tannin-rich browse, and the affinity of the proteins was always similar to the affinity of bovine serum albumin. Our results suggest there is not a major role for salivary tannin-binding proteins in goats. Different tendencies of goat breeds to consume tannin-rich browse does not appear be related to differences in salivary tannin-binding proteins.

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Section: Introductionmentioning

confidence: 85%

No Major Role for Binding by Salivary Proteins as a Defense Against Dietary Tannins in Mediterranean Goats

et al. 2010

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“…Different ruminant feeding styles (browser vs grazer) were related to differences in salivary proteome. For example goats and sheep differ in the expression level of several parotid salivary proteins, such as one isoform of carbonic anhydrase VI and some isoforms of clusterin precursor, among others (Lamy et al 2008(Lamy et al , 2009.…”

Section: Assessing Diet Composition and Voluntary Food Intake In Montadomentioning

confidence: 99%

“…The affinity to bind tannins has been mostly associated with proline rich proteins (PRPs), which are the most well studied among the TBSPs (Shimada 2006). Lamy et al (2008Lamy et al ( , 2009 were unable to identify PRPs in parotid saliva from sheep and goat breeds, commonly present in Montado. In the case of goats, which usually ingest higher levels of tannins, comparatively to sheep, this may suggest the existence of other defense mechanisms.…”

Section: Assessing Diet Composition and Voluntary Food Intake In Montadomentioning

confidence: 99%

Assessing foraging strategies of herbivores in Mediterranean oak woodlands: a review of key issues and selected methodologies

Ferraz-de-Oliveira

Lamy

Bugalho³

et al. 2013

Agroforest Syst

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Montados are agro-silvo-pastoral ecosystems, typical of the Southwest Iberian Peninsula, of high socio-economic and conservation importance, where grazing is a dominant activity. Montados are characterized by an open tree canopy of Quercus sp. and a diverse undercover of shrubs and grasslands that constitute the plant food resources for grazing animals. Plant food resources of Montados are highly variable, both spatially and seasonally, in quantity and quality. Reliable and easy to use methods to monitor grazing are necessary to allow proper understanding of foraging strategies of grazing animals and to set sustainable grazing management. We describe the main characteristics of the plant food resources available for grazing animals, striking its variability, and revise the potential of using N-alkanes and saliva proteome methods to assess foraging strategies in Montados. In a scenario of dynamic multiple choices, we discuss the use of n-alkane methodology for the simultaneous estimation of diet composition and voluntary intake and saliva proteome as a mean of increasing the knowledge on diet adjustments.

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“…In goats, only a few studies have investigated the TBSP. Lamy et al (2008) found major differences in saliva protein within the range of 25-35 kDa and hypothesized their relation with differences on feeding behaviour comparatively to sheep. No PRPs were found either in sheep or in goats maintained on a regular tannin-free diet (Lamy et al 2009).…”

Section: Introductionmentioning

confidence: 94%

Do goats have a salivary constitutive response to tannins?

Ventura-Cordero

Sandoval-Castro

Torres-Acosta

et al. 2015

Journal of Applied Animal Research

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The effect of tannin-rich fodder (TRF) consumption on the saliva response of Criollo goats without previous browsing experience was determined. Eighteen kids were allocated into three treatments (n = 6 each): control group (CG), short-term tannin stimulus (TS) and long-term tannin stimulus (TL). Three experimental periods were used: adaptation (two weeks) in which the three treatment groups were fed Pennisetum purpureum grass and supplemented with a balanced feed. In period 1 (five weeks), TS and TL treatments were fed TRF (Lysiloma latisiliquum) with 55.5 g/kg DM condensed tannins (CT), grass and balanced feed. In period 2 (three weeks), L. latisiliquum fodder was withdrawn from the TS group. Thus, both the TS and the CG kids were only offered grass and balanced feed, while TL kids continued to receive TRF. In each period, saliva samples were collected to measure the tannin-protein interaction, salivary protein and protein turbidity index (PTI). The salivary protein was similar in all treatments and periods (P > .10). The goats' saliva reacted similarly when mixed with either tannic acid or L. latisiliquum water-acetone extract. Although the PTI tended to increase in the TL group compared to CG and TS with time, such difference was not significant. Thus, TRF intake failed to further increase the salivary response or PTI. Therefore, the saliva of goats from Yucatan, without previous browsing experience do have a constitutive response as it can block tannins irrespective of TRF stimulus. ARTICLE HISTORY

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Comparison of Electrophoretic Protein Profiles from Sheep and Goat Parotid Saliva

Cited by 38 publications

References 56 publications

No Major Role for Binding by Salivary Proteins as a Defense Against Dietary Tannins in Mediterranean Goats

No Major Role for Binding by Salivary Proteins as a Defense Against Dietary Tannins in Mediterranean Goats

Assessing foraging strategies of herbivores in Mediterranean oak woodlands: a review of key issues and selected methodologies

Do goats have a salivary constitutive response to tannins?

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