Comparison of Gut Microbiota Diversity and Predicted Functions Between Healthy and Diseased Captive Rana dybowskii

Tong, Qing; Cui, Li-Yong; Du, Xiaodong; Hu, Zongfu; Jia, Bingrui; Xiao, Jianhua; Wang, Hongbin; Zhang, Jiantao

doi:10.3389/fmicb.2020.02096

Cited by 35 publications

(27 citation statements)

References 59 publications

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“…Moreover, captive lemurs from the DLC and NCZ in the U.S. had some of the most diverse gut consortia, equaling the greatest diversity seen in wild lemurs (e.g., in BEZ lemurs). These results add to the mounting evidence 61,82,83 that alpha diversity alone, without the context of host ecology and other microbiome data, should not be used to extrapolate about the health state of gut consortia or the host’s environment.…”

Section: Discussionmentioning

confidence: 60%

Gut microbiota of ring-tailed lemurs (Lemur catta) vary across natural and captive populations and correlate with environmental microbiota

Bornbusch

et al. 2021

Preprint

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Background: Inter-population variation in host-associated microbiota reflects differences in the hosts' environments, but this characterization is typically based on studies comparing few populations. The diversity of natural habitats and captivity conditions occupied by any given host species has not been captured in these comparisons. Moreover, intraspecific variation in gut microbiota, generally attributed to diet, may also stem from differential acquisition of environmental microbes - an understudied mechanism by which host microbiomes are directly shaped by environmental microbes. To more comprehensively characterize gut microbiota in an ecologically flexible host, the ring-tailed lemur (Lemur catta; n = 209), while also investigating the role of environmental acquisition, we used 16S rRNA sequencing of lemur gut and soil microbiota sampled from up to 13 settings, eight in the wilderness of Madagascar and five in captivity in Madagascar or the U.S. Based on matched fecal and soil samples, we used source-sink ecological theory to examine covariation between the two types of consortia. Results: The diversity of lemur gut microbes varied markedly within and between settings. Microbial diversity was not consistently greater in wild than in captive lemurs, indicating that this metric is not necessarily an indicator of host habitat or condition. Variation in microbial composition was inconsistent with either a single, representative gut community for wild conspecifics or a universal 'signal of captivity' that homogenizes the gut consortia of captive animals. Despite the similar, commercial diets of captive lemurs on both continents, lemurs within Madagascar were compositionally most similar, suggesting that non-dietary factors govern some of the variability. In particular, soil microbial communities were most distinct between the two continents, and there was significant and context-specific covariation between lemur gut and soil microbiota. Conclusions: As one of the broadest, single-species investigations of primate microbiota, our study highlights that gut consortia are sensitive to multiple scales of environmental differences. This finding begs a reevaluation of the simple 'captive vs. wild' dichotomy. Beyond the important implications for animal care, health, and conservation, our finding that environmental acquisition may mediate aspects of host-associated consortia further expands the framework for how host-associated and environmental microbes interact across different microbial landscapes.

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Section: Discussionmentioning

confidence: 60%

Gut microbiota of ring-tailed lemurs (Lemur catta) vary across natural and captive populations and correlate with environmental microbiota

Bornbusch

et al. 2021

Preprint

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“…6). However, studies have shown remarkable differences in the gut microbiota between healthy and ill animals (e.g., R. dybowskii), and a signi cant positive correlation exists between the community and functional composition, indicating that the composition of the gut bacterial community in diseased animals has lower functional redundancy [14,18]. The degree of dysbiosis caused by antibiotics and diarrhoea in the gut microbiota may differ.…”

Section: The Effect Of An Antimicrobial Bath On the Function Of The Gmentioning

confidence: 99%

“…Diarrhoea is also probably followed by the disappearance of functional redundancy, which may be related to the severity and progression of dysbiosis [17]. The functional redundancy of the same species may differ among developing statuses [18]. For example, gut redundancy in infants may largely surpass that in adults [17].…”

Section: The Effect Of An Antimicrobial Bath On the Function Of The Gmentioning

confidence: 99%

“…Functional redundancy heavily affects the consequences of orbits caused by differences in age, diets, and diseases [17]. Gut microbiotas signi cantly differ between normal and diseased animals (e.g., frogs and shrimps), and the community is signi cantly and positively correlated with the functional composition [14,18]. Disease may also be companied by the disappearance of functional excess, which is likely related to the intensity and advancement of dysbiosis [17].…”

Section: Introductionmentioning

confidence: 99%

“…Rana dybowskii (brown frog) is a major aquaculture species with medical and nutritional values in China [18]. The current stagnation of the frog culture industry is mainly caused by diseases, primarily bacterial diseases [12].…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Tong

Cui

Jia

et al. 2021

Preprint

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Background: Amphibians frequently receive an antibiotic bath after feedlot placement to control bacterial diseases. The potential collateral effect of these antibiotics on the frog microbiota is largely unknown. Antibiotics are frequently employed to examine the role of the gut microbiota. Existing research relies mainly on oral antibiotics, but knowledge regarding the effects of antibiotics on the gut microbiota through a bath or local antimicrobial therapies is limited. Results: The gut microbiota of gentamicin, recovered, and control Rana dybowskii groups were compared by Illumina high-throughput sequencing, and the functional profiles were analysed using a phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt). Furthermore, the relationship between gut microbiota structures and forecast function compositions was determined. The results showed that the alpha diversity indices were significantly reduced by the gentamicin bath, which significantly changed the composition of the gut microbiota. After 7 days, the gut microbiota was still similar to that during the gentamicin bath. Forty-four indicator species were selected at the genus level, namely, 42 species indicating the control group and 2 species indicating the gentamicin and recovery groups. Potential pathogenic bacteria belonging to Aeromonas, Citrobacter, and Chryseobacterium significantly decreased after the gentamicin bath. The community similarity assays did not show an obvious discrepancy in the functional composition between the gentamicin and control frogs, indicating that the functions of the gut bacterial community were highly redundant. Conclusions: The gentamicin bath significantly reduced the alpha diversity of the gut microbiota of R. dybowskii. Gentamicin significantly changed the structure of the gut microbiota, and the gut microbiotas exhibited weak resilience and did not totally recover after seven days. The gentamicin bath did not change the functional composition of the gut microbiota of R. dybowskii, and there was no significant correlation between the composition of the gut microbiota and the functional composition, illustrating the high intestinal functional redundancy of the frog gut bacterial community. This work offers basic data for upcoming research, including the establishment of the amphibian gut microbiota and local antibiotic administration, and has important implications for aquaculture management and amphibian conservation.

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The succession of gut microbiota in the concave‐eared torrent frog (Odorrana tormota) throughout developmental history

Shi

Deng

et al. 2023

Ecology and Evolution

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Comparison of Gut Microbiota Diversity and Predicted Functions Between Healthy and Diseased Captive Rana dybowskii

Cited by 35 publications

References 59 publications

Gut microbiota of ring-tailed lemurs (Lemur catta) vary across natural and captive populations and correlate with environmental microbiota

Gut microbiota of ring-tailed lemurs (Lemur catta) vary across natural and captive populations and correlate with environmental microbiota

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

The succession of gut microbiota in the concave‐eared torrent frog (Odorrana tormota) throughout developmental history

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