2009
DOI: 10.1016/j.jviromet.2009.03.005
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of immunoassay and real-time PCR methods for the detection of Jembrana disease virus infection in Bali cattle

Abstract: 1A sensitive diagnostic assay for the detection of infections with the bovine 2 lentivirus Jembrana disease virus (JDV) is required in Indonesia to control the 3 spread of Jembrana disease. Immunoassays are used routinely but are 4 compromised by cross-reactive epitopes in the capsid (CA) protein of JDV 5 and the genetically related bovine immunodeficiency virus (BIV). JDV gag-6 specific primers were tested in a real-time PCR assay to detect proviral DNA 7 in peripheral blood mononuclear cells from 165 cattle … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
3
0
1

Year Published

2010
2010
2020
2020

Publication Types

Select...
4
1
1

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(4 citation statements)
references
References 37 publications
0
3
0
1
Order By: Relevance
“…Differentiating BIV infections is feasibly attained by the use of a specific monoclonal antibody, only recognizing BIV capside protein epitope that is absent in JDV (Zheng et al, 2001;Lu et al, 2002). Though relatively insensitive, ELISA provides an economical and feasible method for monitoring the virus in the absence of more sensitive methods (Barboni et al, 2001;Lewis et al, 2009).…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Differentiating BIV infections is feasibly attained by the use of a specific monoclonal antibody, only recognizing BIV capside protein epitope that is absent in JDV (Zheng et al, 2001;Lu et al, 2002). Though relatively insensitive, ELISA provides an economical and feasible method for monitoring the virus in the absence of more sensitive methods (Barboni et al, 2001;Lewis et al, 2009).…”
Section: Resultsmentioning
confidence: 99%
“…JDV antigen detection using ELISA was found to be more sensitive than Western blot (Barboni et al, 2001;Lewis et al, 2009). In addition, latest achievement method in immunodiagnosis is gain by JDVp26 capture ELISA that facilitated monitoring and detection of circulating viral antigen during the acute phase of the disease.…”
Section: Future Directionmentioning
confidence: 99%
See 1 more Smart Citation
“…PCR dilakukan dalam total volume reaksi 25µl yang terdiri dari 8,5 µl Nuclease Free Water, 12,5 µl green master mix, 1 µl Forward primer (JDV 1) dengan sekuen 5'GCAGCGGAGGTGGCAATTTTGATAGG A 3', 1 µl Reverse primer (JDV 3) dengan sekuen 5' CGGCGTGGTGGTCCACCCCATG 3' (Chadwick et al, 1995) (Hartaningsih, 2002). Sel yang menjadi target infeksi dari virus penyakit Jembrana adalah sel limfosit, sehingga untuk mendeteksi keberadaan DNA provirus virus penyakit Jembrana dapat dilakukan dengan mengekstraksi DNA dari PBMC (Tenaya dkk., 2003 (Chadwick et al, 1995, Desport et al, 2007, Lewis et al, 2009. Pita DNA dengan panjang yang sama diperoleh pada kontrol positif pada penelitian ini.…”
Section: Bahan Dan Metodeunclassified