Comparison of in vivo Human Dental Plaque pH Changes within Artificial Fissures and at Interproximal Sites

Igarashi, Koei; Lee, I K; Schachtele, C F

doi:10.1159/000261220

Cited by 43 publications

(31 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…28 The oral cavity presented very diverse microbiota due to the different anatomical sites and constant exposure to the external environment. The method of collecting saliva without using chewing gum or paraffin tablets gave better results than in previous studies, [34][35][36][37][38] suggesting that the technique used in this study is more sensitive and causes fewer alterations to indigenous flora. Results from the back of the tongue were similar to those of other studies.…”

Section: Discussionmentioning

confidence: 70%

“…Results from the back of the tongue were similar to those of other studies. [35][36][37][38] Periodontal Gracey curettes were preffered for sampling from the supra-and subgingival biofilm. Our results were From a microbiological point of view, the esophagus has always been considered to be a saliva secretion passageway with transitory microbiota similar to the mouth and of little interest in research.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Digestive Tract Microbiota in Healthy Volunteers

Zilberstein

Quintanilha

Santos

et al. 2007

Clinics

139

View full text Add to dashboard Cite

PURPOSE:The aim of this study was to standardize the methods of sample collection of mucus from the digestive tract and to determine the microbiota in healthy volunteers from Brazil, collecting samples from the mouth, esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum. METHODS: Microbiota of selected healthy volunteers from the oral cavity (n=10), the esophagus (n=10), the upper digestive tract (n=20), and the lower digestive tract (n=24) were evaluated through distinct collection methods. Collection methods took into account the different sites, using basic scraping and swabbing techniques, stimulated saliva from the oral cavity, irrigationaspiration with sterile catheters especially designed for the esophagus, a probe especially designed for upper digestive tract, and a special catheter for the lower digestive tract. RESULTS: (i) Mixed microbiota were identified in the oral cavity, predominantly Gram-positive aerobic and anaerobic cocci; (ii) transitional flora mainly in the esophagus; (iii) Veillonella sp, Lactobacillus sp, and Clostridium sp in the stomach and duodenum; (iv) in the jejunum and upper ileum, we observed Bacteroides sp, Proteus sp, and Staphylococcus sp, in addition to Veillonella sp; (v) in the colon, the presence of "nonpathogenic" anaerobic bacteria Veillonella sp (average 10 5 UFC) indicates the existence of a low oxidation-reduction potential environment, which suggests the possibility of adoption of these bacteria as biological markers of total digestive tract health. CONCLUSIONS: The collection methods were efficient in obtaining adequate samples from each segment of the total digestive tract to reveal the normal microbiota. These procedures are safe and easily reproducible for microbiological studies.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Digestive Tract Microbiota in Healthy Volunteers

Zilberstein

Quintanilha

Santos

et al. 2007

Clinics

139

View full text Add to dashboard Cite

show abstract

“…Thus, the ISFET pH electrode enabled us to measure in vivo acid production by human dental plaque (Igarashi et al, 1981;Chida et al, 1986), and artificial fissure plaque (Igarashi et al, 1989(Igarashi et al, , 1990. In addition, the ISFET pH electrode is small enough to assess pH at the interface between parasites and biomaterials, and is suitable for the development of a new apparatus to assess pH at the interface in vitro.…”

mentioning

confidence: 99%

Evaluation of pH at the Bacteria–Dental Cement Interface

et al. 2011

Self Cite

View full text Add to dashboard Cite

Physiochemical assessment of the parasite-biomaterial interface is essential in the development of new biomaterials. The purpose of this study was to develop a method to evaluate pH at the bacteriadental cement interface and to demonstrate physiochemical interaction at the interface. The experimental apparatus with a well (4.0 mm in diameter and 2.0 mm deep) was made of polymethyl methacrylate with dental cement or polymethyl methacrylate (control) at the bottom. Three representative dental cements (glass-ionomer, zinc phosphate, and zinc oxide-eugenol cements) were used. Each specimen was immersed in 2 mM potassium phosphate buffer for 10 min, 24 hrs, 1 wk, or 4 wks. The well was packed with Streptococcus mutans NCTC 10449, and a miniature pH electrode was placed at the interface between bacterial cells and dental cement. The pH was monitored after the addition of 1% glucose, and the fluoride contained in the cells was quantified. Glass-ionomer cement inhibited the bacteria-induced pH fall significantly compared with polymethyl methacrylate (control) at the interface (10 min, 5.16 ± 0.19 vs. 4.50 ± 0.07; 24 hrs, 5.20 ± 0.07 vs. 4.59 ± 0.11; 1 wk, 5.34 ± 0.14 vs. 4.57 ± 0.11; and 4 wks, 4.95 ± 0.27 vs. 4.40 ± 0.14), probably due to the fluoride released from the cement. This method could be useful for the assessment of pH at the parasite-biomaterial interface.

show abstract

“…In this study, a series of sucrose pulses, followed by rinsing with artificial saliva, was used to model the situation which occurs in vivo during the consumption of sucrose-rich drinks (11,12), an approach which has been previously used to expose dental plaque to a cariogenic challenge (8,9). Sucrose availability affects the composition and structure of oral biofilms (17,19).…”

mentioning

confidence: 99%