Comparison of laccase production levels in Pichia pastoris and Cryptococcus sp. S-2

Nishibori, Nahoko; Masaki, Kazuo; Tsuchioka, Hiroaki; Fujii, Tsutomu; Iefuji, Haruyuki

doi:10.1016/j.jbiosc.2012.10.025

Cited by 18 publications

(11 citation statements)

References 25 publications

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“…Summaries of heterologously expressed laccases can be found in recent publications (Kittl et al, 2012; Mate and Alcalde, 2015; Antošovǎ and Sychrová, 2016; Ergün and Çalık, 2016). Occasionally, high recombinant laccase activity is obtained (Hong et al, 2002, 2007; Nishibori et al, 2013), but many recombinant laccases are expressed at levels below 10 U/mL, which can be even lower than that in the native strain (Yang et al, 2016b). An appropriate host is needed for heterologous expression of laccases, but “the best host” remains elusive (Piscitelli et al, 2010; Rivera-Hoyos et al, 2013).…”

Section: Classical and Molecular Breeding For Enhancing Laccase Produmentioning

confidence: 99%

“…The expression advantage is likely due to similar codon usage and GC content of Cryptococcus sp. S-2 with those of T. versicolor and G. graminis (Nishibori et al, 2013). Nonetheless, different codon preferences of the expression host and the gene source fails to explain the variability in production yields between laccase genes derived from the same organism (Piscitelli et al, 2010).…”

Section: Classical and Molecular Breeding For Enhancing Laccase Produmentioning

confidence: 99%

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Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas. There have been over two dozen reviews on laccases since 2010 covering various aspects of this group of versatile enzymes, from their occurrence, biochemical properties, and expression to immobilization and applications. This review is not intended to be all-encompassing; instead, we highlighted some of the latest developments in basic and applied laccase research with an emphasis on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics. Pharmaceuticals are a broad class of emerging organic contaminants that are recalcitrant and prevalent. The recent surge in the relevant literature justifies a short review on the topic. Since low laccase yields in natural and genetically modified hosts constitute a bottleneck to industrial-scale applications, we also accentuated a genus of laccase-producing white-rot fungi, Cerrena, and included a discussion with regards to regulation of laccase expression.

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Section: Classical and Molecular Breeding For Enhancing Laccase Produmentioning

confidence: 99%

Section: Classical and Molecular Breeding For Enhancing Laccase Produmentioning

confidence: 99%

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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“…However, both of our native and codon-optimized constructs of AnBX were predicted to contain similar mRNA secondary structures by Mfold Web Server (http:// mfold.rna.albany.edu/?q=mfold). On the other hand, Nishibori et al [40] reported that using the host with similar GC content to the target protein could improve protein expression level. In our case, the GC content of the AnBX gene was found to be 57.68%, which could be too high for a high expression level in P. pastoris, whose GC content was 41.90% [41].…”

Section: Discussionmentioning

confidence: 99%

Cloning, expression and characterization of β-xylosidase from Aspergillus niger ASKU28

Choengpanya

Arthornthurasuk

Wattana-Amorn

et al. 2015

Protein Expression and Purification

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“…22 So far, recombinant laccases have been mostly produced in Saccharomyces cerevisiae [23][24][25] and Pichia pastoris [26][27][28] but the use of Kluyveromyces lactis, 29 Yarrowia lipolytica, 30 Pichia methalonica, 31 and Cryptococcus sp. 32 has also been described.…”

Section: Introductionmentioning

confidence: 98%

“…Other frequently used hosts for laccase production are yeasts, due to their rapid growth, efficient secretion machinery, and a large set of tools for genetic engineering . So far, recombinant laccases have been mostly produced in Saccharomyces cerevisiae and Pichia pastoris but the use of Kluyveromyces lactis , Yarrowia lipolytica , Pichia methalonica , and Cryptococcus sp . has also been described.…”

Section: Introductionmentioning

confidence: 99%

Efficient secretion of three fungal laccases from Saccharomyces cerevisiae and their potential for decolorization of textile industry effluent—A comparative study

Antošová

Herkommerová

Pichová

et al. 2017

Biotechnology Progress

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Laccases are enzymes with a broad range of biotechnological applications and have, for example, the ability to oxidize many xenobiotics including synthetic dyes. In order to obtain an efficient laccase for the decolorization of dyes which spoil wastewater from the textile industry, genes encoding three various laccase enzymes were expressed in Saccharomyces cerevisiae. The expression of laccases from ascomycete Myceliophthora thermophila (MtL), and two basidiomycetes Trametes versicolor (TvL) and Trametes trogii (TtL) was optimized via selection of plasmids, promoters, media composition, and cultivation conditions. For the first time, the activity of the three secreted laccases was directly compared with the use of various substrates, including different dyes and a wastewater sample. A strong constitutive ADH1 promoter, minimal growth medium, optimized combination of copper and organic nitrogen source, and low cultivation temperature were shown to significantly increase the yields and relative activities of secreted laccases. Heterologous expression of three fungal laccases was successfully achieved in S. cerevisiae being the highest for MtL and the lowest for TvL. MtL, and particularly TtL, showed the decolorization capacity. This is the first report which compared decolorization of synthetic dyes and wastewater by several recombinant laccases and suggested MtL and TtL to be applicable in the ecofriendly enzymatic treatment of colored industry effluent. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:69-80, 2018.

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Comparison of laccase production levels in Pichia pastoris and Cryptococcus sp. S-2

Cited by 18 publications

References 25 publications

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Cloning, expression and characterization of β-xylosidase from Aspergillus niger ASKU28

Efficient secretion of three fungal laccases from Saccharomyces cerevisiae and their potential for decolorization of textile industry effluent—A comparative study

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