Comparison of Ligation-Mediated PCR Methods in Differentiation of<i>Mycobacterium tuberculosis</i>Strains

Żaczek, Anna; Brzostek, Anna; Wojtasik, Arkadiusz; Sajduda, Anna; Dziadek, Jarosław

doi:10.1155/2014/782071

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…In the present study, Mtb clinical isolates were compared by means of two LM-PCR methods: FLiP (Reisig, 2005) and FLAP (Zaczek et al, 2014a;2014b) and the obtained results were verified by the "gold" epidemiological standard IS6110-RFLP hybridization technique.…”

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confidence: 97%

“…Subsequently, isolates were characterized by IS6110-RFLP typing using internationally standardized protocol (van Embden et al, 1993). The FLAP method was performed as described previously (Zacz ek et al, 2014a;2014b) and the FLiP analysis was performed as originally described by Reisig (Reisig et al, 2005). The fingerprint patterns obtained by these three methods were compared visually with one another and strains were considered identical if their DNA profiles, obtained by all three methods, were the same.…”

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confidence: 99%

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Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

Żaczek

Szwaja²,

Skiba³

et al. 2015

Pol J Microbiol

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The diversity of Mycobacterium tuberculosis clinical isolates, collected from a single hospital, was analyzed by ligation-mediated PCR techniques: FLiP and FLAP, and hybridization technique, IS6110-RFLP. The isolated strains were divided in terms of location (3 towns of Podkarpackie voivodeship differing in population size) and relationship (8 members of 4 families, each represented by 2 patients). Within each family identical DNA profiles, as well as drug resistance patterns were identified indicating a great chance of transmission of strains within the same family. Identical, or very similar patterns were also shared by strains isolated from unrelated patients living in a very small town (1 200 inhabitants) or hospitalized in the same place and time.

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confidence: 97%

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confidence: 99%

Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

Żaczek

Szwaja²,

Skiba³

et al. 2015

Pol J Microbiol

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Advances in diagnosis of Tuberculosis: an update into molecular diagnosis of Mycobacterium tuberculosis

Acharya²,

et al. 2020

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Analyses of the Polymorphisms in E. coli Strains Associated with Heat-Shock Proteins Hsp 55 Isolated from Bird Feathers

Cybulska¹,

Mahdi-Oraibi²,

Miskiewicz³

et al. 2018

Application of Genetics and Genomics in Poultry Science

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The bird feathers are often colonized by pathogenic microorganisms including mainly bacteria of the E. coli species. There is a grooving evidence that due to colonization of the pathogenic bacteria after slaughter material may lead to different zoonosis diseases that endanger human health. Poultry diseases are a very important issue both economically and epidemiologically in each country. Currently, in practice, EU postmortem monitoring programs are often used to eliminate breeding poultry infected with different pathogenic microorganisms, e.g., E. coli by introducing mandatory bird vaccination. The article describes the combination of genetic and genomic methods that were used in the analysis of species specificity of strains and their genomes, including specific pathogenic bacteria in bird feathers. The aim of the study was (i) to investigate DNA polymorphisms of the obtained bacterial strains isolated from avian feathers (ii) obtaining recombinant Hsp55 protein and defining its role as a potential component of vaccines used in poultry diseases. For the detection and analysis of DNA polymorphisms, we have optimized a new innovative method based on the deficiencies of three molecular techniques, AFLP, PCR-MP, and PCR MP. This new method can be a useful tool used in the genotyping of bacterial E. coli serotypes present on avian feathers after the slaughter process. It also allows to effectively identify a number of early stages of infectious diseases from heterogeneous avian research material. Amplification of polymorphic regions was achieved by using a lower denaturation temperature of the primers and a reduction in the number of cycles in the classical PCR, which simplifies the procedure, preserving the quality and reproducibility of the obtained results. Research of recombinant Hsp55 protein has allowed us to determine the optimal conditions for its production by the classical methods used in proteomic analysis.

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Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains

Cited by 3 publications

References 16 publications

Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

Advances in diagnosis of Tuberculosis: an update into molecular diagnosis of Mycobacterium tuberculosis

Analyses of the Polymorphisms in E. coli Strains Associated with Heat-Shock Proteins Hsp 55 Isolated from Bird Feathers

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