Comparison of modulation of sheep, mouse and buffalo lymphocyte responses by Fasciola hepatica and Fasciola gigantica excretory-secretory products

Zhang, Wanlong; Moreau, Emmanuelle; Peigné, F.; Huang, Weiyi; Chauvin, Alain

doi:10.1007/s00436-005-1306-x

Cited by 33 publications

(19 citation statements)

References 21 publications

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“…This finding is indicative of a persistent immune suppression. All the above findings are in agreement with the findings of other studies of different host species that have included infected rats but that have been performed at earlier stages of infection [19][20][21][22][23][24][25][26]. The reason for this unresponsiveness is not yet known.…”

Section: Discussionsupporting

confidence: 82%

Immune Suppression in Advanced Chronic Fascioliasis: An Experimental Study in a Rat Model

et al. 2007

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Today, fascioliasis is considered to be an important human disease caused by 2 liver fluke species, Fasciola hepatica and Fasciola gigantica (Fasciolidae), infecting the liver of a wide range of mammals [1] that show a marked variability in their immune response against infection [2]. There are several geographic regions that have been reported to be areas of hypoendemicity, mesoendemicity, and hyperendemicity for fascioliasis in

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Section: Discussionsupporting

confidence: 82%

Immune Suppression in Advanced Chronic Fascioliasis: An Experimental Study in a Rat Model

et al. 2007

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“…The proliferation of cells was estimated by 5-bromo 2Ј-deoxyuridine (BrdU) incorporation using a colorimetric kit (Roche Diagnostics, Germany). The stimulation index (SI) was calculated as a ratio of the mean optical density at 450 nm of stimulated cultures to that of unstimulated cultures (60).…”

Section: Methodsmentioning

confidence: 99%

Role for Nitric Oxide in Hookworm-Associated Immune Suppression

Dondji¹,

Bungiro²,

Harrison³

et al. 2008

Infect Immun

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Hookworm infection is a major cause of anemia and malnutrition in resource-poor countries. Human and animal studies suggest that infection with these intestinal nematodes is associated with impaired cellular immunity, characterized by reduced lymphocyte proliferation in response to both parasite and heterologous antigens. We report here data from studies aimed at defining mechanisms through which hookworms modulate the host cellular immune response. Splenocytes and mesenteric lymph node (MLN) cells from hamsters infected with Ancylostoma ceylanicum showed minimal proliferation in response to mitogen at days 20 and 30 postinfection (p.i.), with partial recovery noted at day 70 p.i. The proliferative capacity of enriched splenocyte T-cell preparations from infected animals following stimulation with hookworm antigens was partially restored in the presence of antigen-presenting cells from uninfected hamsters. Analysis by fluorescence-activated cell sorting revealed that hookworm infection is associated with reduced percentages of both CD4؉ and surface immunoglobulin G-positive lymphocytes in the spleen and MLN cells. Splenocytes from infected hamsters also secreted more nitric oxide (NO) in culture than did those from naïve animals. Inhibition of NO secretion was associated with partial restoration of the proliferative capacity of splenocytes from infected animals in response to concanavalin A, suggesting a role for NO in mediating this effect. Together, these data demonstrate that hookworm infection is associated with impaired function of antigen-presenting cells and depletion of important lymphocyte subpopulations and also suggests a role for NO in parasite-induced immunosuppression.It is estimated that more than 700 million people in resourcepoor countries are infected with hookworms, bloodfeeding intestinal nematodes that cause anemia and malnutrition (8,14). Together with Ascaris lumbricoides and Trichuris trichiura, the hookworms Ancylostoma duodenale and Necator americanus comprise the group of soil-transmitted nematodes that are now recognized as a major cause of global morbidity (2, 56). Significant clinical features of hookworm infection in humans include irondeficiency anemia, hypoproteinemia, and growth delay (13, 53). Although control strategies relying on targeted delivery of benzimidazole antihelminthics are generally effective at eliminating adult worms, reinfection occurs quickly and frequent treatments may be necessary for sustained improvement in the health of at-risk populations (50,52).Although sterile immunity does not appear to develop following natural infection, data from human and animal studies confirm that hookworms elicit humoral and cellular immune responses in mammalian hosts (18). Although the nature of this response has yet to be elucidated fully, infection appears to be associated with a mixed Th1/Th2 host cytokine profile (22,49). It has also been reported that hookworm infection is associated with suppression of host cellular responses to hookworm-specific and heterologous antige...

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“…Menurut Nambi et al (2005), respons humoral dan seluler kerbau (Bubalus bubalis) meningkat signifikan dua minggu setelah diimunisasi dengan 400 μg ekskretori/sekretori Fasciola gigantica (F. gigantica). Sifat antigenik ekskretori/sekretori cacing telah dibuktikan oleh peneliti terdahulu (Vervelde et al, 2003;Paz-Silva et al, 2003;Paz-Silva et al, 2004;Zhang et al, 2005;dan Yadav et al, 2005). Aktivitas antigenik ekskretori/sekretori Fasciola hepatica (F. hepatica) dan F. gigantica dibuktikan pula oleh dan Paz-Silva et al (2004) dan Zhang et al (2005).…”

Section: Hasil Dan Pembahasanunclassified

“…Sifat antigenik ekskretori/sekretori cacing telah dibuktikan oleh peneliti terdahulu (Vervelde et al, 2003;Paz-Silva et al, 2003;Paz-Silva et al, 2004;Zhang et al, 2005;dan Yadav et al, 2005). Aktivitas antigenik ekskretori/sekretori Fasciola hepatica (F. hepatica) dan F. gigantica dibuktikan pula oleh dan Paz-Silva et al (2004) dan Zhang et al (2005). Aktivitas tersebut dapat menimbulkan efek imunomodulator dan memicu proliferasi limfosit domba, tikus, dan kerbau.…”

Section: Hasil Dan Pembahasanunclassified

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli

et al. 2013

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ABSTRAKPenelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P<0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli. ____________________________________________________________________________________________________________________ Kata kunci: Ascaridia galli, ekskretori/sekretori, antibodi, ELISA ABSTRACT

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Comparison of modulation of sheep, mouse and buffalo lymphocyte responses by Fasciola hepatica and Fasciola gigantica excretory-secretory products

Cited by 33 publications

References 21 publications

Immune Suppression in Advanced Chronic Fascioliasis: An Experimental Study in a Rat Model

Immune Suppression in Advanced Chronic Fascioliasis: An Experimental Study in a Rat Model

Role for Nitric Oxide in Hookworm-Associated Immune Suppression

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli

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