Comparison of neomycin production from Streptomyces fradiae cultivation using soybean oil as the sole carbon source in an air-lift bioreactor and a stirred-tank reactor

Ohta, Noriyasu; Park, Yong Soo; Yahiro, Kazutoyo; Okabe, Mitsuyasu

doi:10.1016/0922-338x(95)91259-8

Cited by 29 publications

(21 citation statements)

References 10 publications

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“…Mycelium under a low agitation rate showed hyphal growth, branched, twined, and formed pellets (Ohta et al, 1995). However, mycelia length and twined mycelia under a high agitation rate became shorter and fewer, respectively, than those under a low agitation rate (Ohta et al, 1995). In this research, when 30 g/L of support was used, the viscosity increased threefold over that with 15 g/L of support (see Fig.…”

Section: Discussionmentioning

confidence: 81%

“…Moreover, an increase of the viscosity in the culture broth causes morphological changes of the mycelium. Mycelium under a low agitation rate showed hyphal growth, branched, twined, and formed pellets (Ohta et al, 1995). However, mycelia length and twined mycelia under a high agitation rate became shorter and fewer, respectively, than those under a low agitation rate (Ohta et al, 1995).…”

Section: Discussionmentioning

confidence: 98%

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Application of mineral support on cephamycin C production in culture using soybean oil as the sole carbon source

Mitsuru

Park

Kosakai

et al. 1997

Biotechnol. Bioeng.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 98%

Application of mineral support on cephamycin C production in culture using soybean oil as the sole carbon source

Mitsuru

Park

Kosakai

et al. 1997

Biotechnol. Bioeng.

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“…Rapeseed oil and soybean oil are two commonly used oils for antibiotic fermentations and, when used as the sole carbon source in a batch process, these oils are typically added at between 2% and 6% (v/v) (Choi et al, 1996;Ohta et al, 1995;Park et al, 1994). Because the overall rate of oil mass transfer in the fermentor and/or the limitation in the rate of lipid utilization is strongly affected by the drop size/interfacial area, it is generally considered necessary to disperse the oil into relatively small drops (Large et al, 1998;Mitsuru et al, 1997;Ohta et al, 1995). However, even if oil is well dispersed, large amounts of residual oil at the end of fermentation are frequently found, which is a major disadvantage (Large et al, 1998).…”

Section: Fermentation Aspectsmentioning

confidence: 99%

Oil/water and pre‐emulsified oil/water (PIT) dispersions in a stirred vessel: Implications for fermentations

Erler

Nienow²,

Pacek³

2003

Biotech & Bioengineering

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This study examines dispersions of rapeseed oil (RSO) in water by mechanical agitation under conditions mimicking those found in certain antibiotic fermentations; for example, in the presence of air, antifoam, and finely divided CaCO(3) particles. A problem with residual oil has been reported for such fermentations, and it has been suggested that the use of pre-emulsified oil can reduce this problem. Hence, the dispersion of a pre-emulsified oil produced by the "phase inversion temperature (PIT) method" has been evaluated. In both cases, the volume fraction of oil was 2%. For the RSO systems, a relatively high agitation speed was required to disperse the oil, especially in the presence of the particles and, when the agitation was stopped, separation occurred rapidly. The Sauter mean drop diameters depended on the system, being at an average energy dissipation rate of approximately 0.9 W kg(-1), 180 microm for RSO/water, 130 microm for RSO/water(antifoam)/air, 580 microm for RSO/water/CaCO(3), and 850 microm for RSO/water(antifoam)/air/CaCO(3). For the same four systems, the PIT emulsion, once dispersed, was very stable and the drop size was essentially independent of the operating conditions, with a Sauter mean diameter of approximately 0.3 microm. The implications of these findings for fermentations in which oil is used as a carbon source are assessed.

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“…Some authors have reported optimum results when oil was added frequently during fermentation as a sole carbon source (Gibson and Coughlin, 2002;Jones and Porter, 1998;Ohta et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Lipids and oils are considered essential components of many fermentation media since they have defoaming properties and serve as a supplemental nutrient source for growth and also maintenance of the microbial cells (Ohta et al, 1995). Some authors have reported optimum results when oil was added frequently during fermentation as a sole carbon source (Gibson and Coughlin, 2002;Jones and Porter, 1998;Ohta et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of pullulan by two strains of A. pullulans with different concentrations of soybean oil in sucrose solution in batch fermentations

Sena

Costelli

Gibson

et al. 2006

Braz. J. Chem. Eng.

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-Aureobasidium pullulans is a microorganism that produces pullulan (homopolysaccharide) extracellularly through a fermentation process with sugars (maltose, d-xylose, sucrose and starch) as its carbon source. Pullulan is a linear polysaccharide of D-glycopyranose containing (1 → 4)-α and (1 → 6)-α linkages at a 2:1 ratio, is highly soluble in water and has various applications in the food, packaging, film and pharmaceutical industries. Lipids, primarily oils, having antifoaming properties as well as nutritional particularities, are considered an essential additional carbon source for the growth of microorganisms, especially fungi. These nutrient sources are very important for the maintenance of microorganism cells. In fact, these positive effects are only achieved when the right source is added at both the right time and the right dosage into the broth of the fermentation process. In this research on pullulan production with the strains NRRL Y-6220 and NRRL Y-2311-1, it was found that the latter strain achieved better results for undesirable pigment formation, pullulan titer, time of maximum production (96 hours) and biomass yields than strain NRRL Y-6220, which also showed suitable results for biomass yields and cell morphology. However, the dark pigmentation of the strain NRRL Y-6220, formed through the process, makes its application unacceptable for foods and pharmaceuticals. Strain NRRL Y-2311-1 was shown to be a promising potential industrial microorganism, whose applications should be studied more in depth.

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Comparison of neomycin production from Streptomyces fradiae cultivation using soybean oil as the sole carbon source in an air-lift bioreactor and a stirred-tank reactor

Cited by 29 publications

References 10 publications

Application of mineral support on cephamycin C production in culture using soybean oil as the sole carbon source

Application of mineral support on cephamycin C production in culture using soybean oil as the sole carbon source

Oil/water and pre‐emulsified oil/water (PIT) dispersions in a stirred vessel: Implications for fermentations

Enhanced production of pullulan by two strains of A. pullulans with different concentrations of soybean oil in sucrose solution in batch fermentations

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