Comparison of Physicochemical Properties of Nano‐ and Microsized Crystals in the Urine of Calcium Oxalate Stone Patients and Control Subjects

Gao, Jie; Xue, Jun-Fa; Xu, Meng; Gui, Bao‐Song; Wang, Fengxin; Ouyang, Jing

doi:10.1155/2014/790473

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…On the other hand, random distribution of the uric acid crystallites observed in the CARS images suggests that they adhered to the already formed stones in the urinary tract. Solubility of uric acid in water is low, and, subsequently, uric acid microcrystallites may be present in urine of even healthy humans . During formation of stones in urinary tract, the crystallites might be incorporated into the stones' structure.…”

Section: Resultsmentioning

confidence: 99%

Microstructure of urinary stones as studied by means of multimodal nonlinear optical imaging

Pučetaitė

Tamošaitytė

Galli

et al. 2016

J Raman Spectroscopy

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Tracing aetiology and pathogenesis of urinary stone disease is of great importance in order to prescribe appropriate treatment and prevent recurrences. For this purpose, morphological examination combined with determination of chemical composition of urinary stones is fundamental. In this work, we have evaluated the potential of multimodal nonlinear optical imaging for investigation of (micro)structure and chemical composition of human urinary stones. The method provides high‐resolution multimodal images of the cross‐sectioned stones without any labelling or some other pretreatment of the samples. We have shown that various constituents of urinary stones can be well discriminated in the multimodal images according to their optical signals. In addition, small structures (1–5 µm in size) were observed in the cross‐sections of urinary stones of various types. These structures were identified as crystallites of uric acid. They could either act as an active element during formation of urinary stones or, more probably, be accidentally incorporated into their structure, as it is suggested by random distribution of the crystallites. The results of this work show that multimodal nonlinear optical imaging can provide relevant information about growth processes of urinary stones and deliver useful insights in aetiology and pathogenesis of urolithiasis. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Microstructure of urinary stones as studied by means of multimodal nonlinear optical imaging

Pučetaitė

Tamošaitytė

Galli

et al. 2016

J Raman Spectroscopy

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“…Crystal size may also be an important factor in cell injury, as recent studies have shown that CaOx nanocrystals cause more cytotoxic effects than microcrystals on renal epithelial cells. 25 Furthermore, nano-scaled crystallites isolated from CaOx stone formers have been shown to more 27 Thus, the ability to accurately detect nanocrystals in the urine could be a useful tool for identifying factors associated with crystal agglomeration and crystal toxicity, and for understanding and managing kidney stone disease. Few studies have detected nanocrystals in the urine using a nanoparticle analyzer and nano-flow cytometer.…”

Section: Discussionmentioning

confidence: 99%

“…Few studies have detected nanocrystals in the urine using a nanoparticle analyzer and nano-flow cytometer. 14,27,28 These methods required labeling crystals with fluorescent probes and dyes for visualization under the microscope or flow cytometry. 28,50 Their findings suggested nanocrystals could be important to understand stone pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Previous investigations have used a range of instruments including a nanoscale flow cytometer, a Zetasizer Nano-ZS with an X-ray diffractometer, and fluorescently labeled bisphosphonate probes to evaluate urinary nanocrystals. 27,28 Limitations of these approaches include the inability to quantify the number of nanocrystals present, the use of nonspecific fluorescent probes, and the need for extensive sample preparation. The purpose of this study was to determine whether (i) urinary nanocrystals can be detected and quantified by NTA (a high-resolution imaging technology), (ii) early void urine samples contain calcium nanocrystals, and (iii) a dietary oxalate load increases urinary nanocrystal formation in healthy subjects.…”

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confidence: 99%

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Dietary Oxalate Induces Urinary Nanocrystals in Humans

Kumar

Patel

Thomas

et al. 2020

Kidney International Reports

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Introduction: Crystalluria is thought to be associated with kidney stone formation and can occur when urine becomes supersaturated with calcium, oxalate, and phosphate. The principal method used to identify urinary crystals is microscopy, with or without a polarized light source. This method can detect crystals above 1 mm in diameter (microcrystals). However, analyses of calcium oxalate kidney stones have indicated that crystallite components in these calculi are 50-100 nm in diameter. Recent studies have suggested that nanocrystals (<200 nm) elicit more injury to renal cells compared to microcrystals. The purpose of this study was to determine whether (i) urinary nanocrystals can be detected and quantified by nanoparticle tracking analysis (NTA, a high-resolution imaging technology), (ii) early-void urine samples from healthy subjects contain calcium nanocrystals, and (iii) a dietary oxalate load increases urinary nanocrystal formation. Methods: Healthy subjects consumed a controlled low-oxalate diet for 3 days before a dietary oxalate load. Urinary crystals were isolated by centrifugation and assessed using NTA before and 5 hours after the oxalate load. The morphology and chemical composition of crystals was assessed using electron microscopy, Fourier-transform infrared spectroscopy (FTIR), and ion chromatography-mass spectrometry (IC-MS). Results: Urinary calcium oxalate nanocrystals were detected in pre-load samples and increased substantially following the oxalate load. Conclusion: These findings indicate that NTA can quantify urinary nanocrystals and that meals rich in oxalate can promote nanocrystalluria. NTA should provide valuable insight about the role of nanocrystals in kidney stone formation.

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“…Что касается витамина С, то его употребление в сутки с продуктами и лекарственными средствами не должно превышать 150-200 мг, поскольку при избыточном употреблении этого витамина растет образование ЩК [35,43,44].…”

Section: диета при обменных нефропатияхunclassified

Metabolic infant nephropathies: a systematic review

Бурлуцкая

Kovalenko

Статова

2021

Kuban. nauсh. med. vestnik

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Background. Metabolic diseases are growing, also in paediatric practice. Metabolic nephropathy is becoming more prevalent comprising 27–64% total paediatric nephrological cases, according to various published evidence. In this concern, the correct diagnosis, dietary and drug therapy acquire particular importance.Objectives. An overview of current methods for laboratory and instrumental diagnosis and treatment of metabolic nephropathies in children.Methods. National and foreign literature sources were mined in the RSCI, VAK, Scopus, Web of Science, PubMed, Cochrane Library and eLibrary databases at a search depth limited to seven years. The query keywords were: metabolic nephropathy [обменная нефропатия], children [дети], calcium oxalate crystalluria [оксалатно-кальциевая кристаллурия], therapy [терапия]. Content and descriptive analyses were used as research tools.Results. The review surveyed 74 literature sources, with 50 selected for further analysis.Discussion. Modern views on metabolic nephropathies are highlighted in terms of classification, clinical progression, methods of diagnosis, treatment and prevention. A current trend in these diseases is earlier-age morbidity. The most studied metabolic nephropathy is calcium oxalate crystalluria. The article also discusses the role of Oxalobacter formigenes bacteria in secondary oxalate nephropathy.Conclusion. Therapies for metabolic nephropathies as polyaetiologic diseases are multicomponent to target both a causal factor and lithogenesis-contributing mechanisms. Current treatment approaches to metabolic nephropathies in children are highlighted in the light of published knowledge.

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Comparison of Physicochemical Properties of Nano‐ and Microsized Crystals in the Urine of Calcium Oxalate Stone Patients and Control Subjects

Cited by 7 publications

References 29 publications

Microstructure of urinary stones as studied by means of multimodal nonlinear optical imaging

Microstructure of urinary stones as studied by means of multimodal nonlinear optical imaging

Dietary Oxalate Induces Urinary Nanocrystals in Humans

Metabolic infant nephropathies: a systematic review

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