Comparison of plasma pharmacokinetics and bioequivalence of ceftiofur sodium in cattle after a single intramuscular or subcutaneous injection

Abstract

Comparison of plasma pharmacokinetics and bioequivalence of ceftiofur sodium in cattle after a single intramuscular or subcutaneous injection. J. vet. Pharmacol. Therap. 23,[273][274][275][276][277][278][279][280] Ceftiofur sodium, a broad-spectrum cephalosporin, is active against gram-positive and gram-negative pathogens of veterinary importance. This study was designed to compare the bioequivalence of the sodium salt in cattle after a single intramuscular (i.m.) or subcutaneous dose (s.c.) of 2.2 mg ceftiofur equivalents/kg body weight. The criteria used to evaluate bioequivalence were (1) the area under the curve from time of injection to the limit of quantitation (LOQ) of the assay (AUC 0-LOQ ), and (2) time concentrations remained above 0.2 mg/mL (t \ 0.2 ).Twelve crossbred beef cattle were enrolled in a three-period, two-treatment crossover trial, with a minimum 2-week washout period between doses of 2.2 mg ceftiofur equivalents/kg. Blood samples were collected serially for up to 72 h post-injection. Plasma samples were then analyzed using a validated assay that measures ceftiofur, and all desfuroylceftiofur-related metabolites, by high-performance liquid chromatography (HPLC) as the stable derivative, desfuroylceftiofur acetamide.A maximum plasma concentration (C max ) of 13.99 3.55 mg/mL was observed from 0.67−2.0 h after i.m. administration, whereas a C max of 13.69 3.85 mg/mL was observed from 0.67 -3.0 h after s.c. administration. The AUC 0-LOQ was 108 9 35.0 mg·h/mL after i.m. dosing, compared with 1059 29.8 mg·h/mL after s.c. dosing. The pre-established criterion for equivalence of the AUC 0-LOQ for the i.m. and s.c. routes of administration was satisfied. The t \ 0.2 was 49.2 98.55 h after i.m. administration, compared with 47.09 9.40 h after s.c. administration. The pre-established criterion for equivalence of the t \ 0.2 for i.m. and s.c. administration was satisfied.The equivalence of AUC 0-LOQ and t \ 0.2 for i.m. and s.c. administration of 2.2 mg ceftiofur equivalents (CE)/kg doses of ceftiofur sodium suggest similar therapeutic efficacy and systemic safety for the two routes of administration.(Paper

Pharmacokinetics of ceftiofur after single intravenous and intramuscular administration in camels (Camelus dromedarius)

“…, 2002). In this study, the peak plasma concentration ( C max ) was 10.34 ± 1.24 μ g/mL, this result is lower than that reported by Brown et al. , 2000 in cattle 13.9 μ g/mL, Brown et al.…”

Pharmacokinetics of intravenous ceftiofur sodium and concentration in body fluids of foals

“…, 1990). The LOQ of this assay has ranged between 0.15 and 0.5 μg/mL (Brown et al. , 2000; Pille et al.…”

Ceftiofur Derivatives in Serum, Uterine Tissues, Cotyledons, and Lochia after Fetal Membrane Retention

“…Pharmacokinetics of ceftiofur have been described for several animals, including cattle (Brown et al, 2000;Okker et al, 2002), pigs (Brown et al, 1999), mares (Jonker, 1997), sheep (Craigmill et al, 1997), goats (Courtin et al, 1997), and llama and alpaca (Drew et al, 2004). In most reports, however, pharmacokinetics were determined for ceftiofur in plasma.…”

“…Although early postpartum use of ceftiofur has been described in several studies, there is only limited information about the pharmacokinetics of ceftiofur in bovine uterine tissue. Pharmacokinetics and bioequivalence of ceftiofur in plasma have been compared for intramuscular and subcutaneous administration (Brown et al, 2000). Previous authors suggested similar therapeutic efficacy for the 2 routes of administration.…”