Comparison of rapid card agglutination test with the complement fixation test for diagnosis of Anaplasma marginale infection in Cattle in Colombia

Todorovic, R. A.; Long, R.F.; McCallon, B.R.

doi:10.1016/0378-1135(77)90006-2

Cited by 10 publications

(9 citation statements)

References 4 publications

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“…Serologic diagnosis of infected cattle is required to identify, isolate, and treat the animals to clear the infection and to prevent transmission to susceptible cattle (21,24). Current diagnostic tests used to detect infected cattle are complement fixation, capillary tube agglutination, rapid card agglutination, and indirect immunofluorescence assays (8, 14,17,18,31). The antigen in all presently used tests is a crude mixture of A. marginale and erythrocyte membranes (10,11).…”

mentioning

confidence: 99%

Detection of an Anaplasma marginale common surface protein present in all stages of infection

Palmer¹,

Barbet²,

Kuttler³

et al. 1986

J Clin Microbiol

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The establishment and maintenance of anaplasmosis-free cattle herds is impaired due to the lack of a rapid, sensitive, and specific serologic test to detect persistently infected cattle which serve as carriers for the organism. To develop an improved diagnostic test for anaplasmosis we screened Anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. Seronegative cattle were infected with either the Florida, Virginia, or North Texas isolate of A. marginale and monitored for infection by daily examination of Wright-stained blood smears for parasitized erythrocytes. Sera from cattle at different stages of infection, from acute through persistent, were used to immunoprecipitate A. marginale proteins that were metabolically radiolabeled with [35S]methionine or surface radiolabeled with 1251. Multiple A. marginale proteins were recognized by using sera either undiluted or gt 1:10; however, only four or five proteins were sufficiently antigenic to elicit antibody reactive with a 1:1,000 serum dilution. A single protein with an apparent molecular mass of 86 kilodaltons was consistently recognized at all stages of infection regardless of the isolate used to infect the cattle. This protein was demonstrated to be on the surface of the A. marginale initial body and to be water soluble. We propose use of this 86-kilodalton protein to develop an improved serologic test for diagnosis of bovine anaplasmosis. Anaplasmosis is a hemoparasitic disease of cattle endemic in half of the livestock production regions worldwide (20).

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mentioning

confidence: 99%

Detection of an Anaplasma marginale common surface protein present in all stages of infection

Palmer¹,

Barbet²,

Kuttler³

et al. 1986

J Clin Microbiol

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“…A definite statement about the occurrence and distribution of this disease can be made only through sero-epidemiologic examination of a representative cross-section of the whole cattle population. This is proven also by numerous treatises from countries involved in similar problems, such as Australia, Canada, the USA, South Africa, and Colombia (BOULANGER et a]., 1966(BOULANGER et a]., , 1971KUTTLER et a]., 1969;LONG et al, 1974;MERRIMAN et al, 1962;PAULL et a]., 1980;POTGIETER, 1979;ROGERS, 1971;TODOROVIC et al, 1977;UTTERBACK et al, 1972;ZAUGG and KUTTLER, 1985).…”

Section: Us Copyright Clearance Center Code Statementmentioning

confidence: 89%

“…Before the conclusive evaluation of the incidence of anaplasmosis in Austria, the phenomenon of fluctuation must be considered, where the CFT titers of subclinically infected animals may vary greatly within a short range of time (HECK et al, 1962;TODOROVIC et al, 1977). The amount of reactors in the Austrian cattle population must be assessed rather higher than in this paper because antibody titers of carriers of anaplasmosis can be detected by means of CFT only within the time period of 98 to 325 days p. i., which makes false-negative reactions more likely (AMERAULT and Roby, 1971;BARRY et al, 1986;GONZALES et al, 1978;ROGERS, 1971;TODOROVIC et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence Survey for Anaplasma marginale‐Infection of Austrian Cattle

Baumgartner

Schlerka

Fumicz

et al. 1992

Journal of Veterinary Medicine, Series B

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Summary A serologic survey study of 5,076 Austrian cattle farming herds was carried out in the period of December 1988 till March 1990. One animal was randomly selected from each herd and the antibody titer against Anaplasma marginale in blood serum samples was evaluated by means of the complement fixation test. The number of these tested blood samples was 3.6% of 140,081 cattle herd farms of Austria. 109 (2.1 %) of the tested animals showed positive titers (1: 10) against Anaplasma marginale, in relation to the 140,081 cattle herds 0.08%. 4,786 (94.3%) blood serum samples were sero‐negative, 188 (3.8 %) reacted anticomplementary. The highest number of antibody‐positive animals of 8 tested Austrian districts could be found in Carinthia (46 = 5.7%). In Burgenland all tested sera turned out to be negative. Concerning the distribution of sero‐positive animals in Austria it can be stated that a decrease of positive reactors from southern to northern region is evident. A connection between the occurrence of anaplasmosis in Italy, Yugoslavia, Switzerland and Hungary, is postulated as a result of the different systems of keeping cattle in the provinces and the regional increase of tick invasion. Possibly an intensive animal transportation is of importance due to the introduction of the disease mentioned before. The results obtained show that anaplasmosis does occur in different areas of Austria. For control of this disease in Austria it is proposed that all imported cattle should be tested serologically for antibodies against Anaplasma marginale. Other diseases in connection with anemia should be excluded by clinical, serological, blood‐, as well as pathological examinations.

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“…Other investigators have noted a lack of clinical disease in Anaplasma-infected North American wild ruminants despite constant parasitemias in splenectomized individuals (Renshaw et al, 1979). However, unlike black-tailed deer (Odocoileus hemionus columbianus) which lost their antibody titers within 16 wk after the peak of parasitemia (Christensen et al, 1958;Osebold et al, 1959), bison maintained CF titers at least 15 mo later, the same was observed with cattle infected with A. marginale (Todorovic et al, 1977). This immunological similarity may be due to the closer taxonomic relationship bison have with cattle than do the cervids.…”

Section: Discussionmentioning

confidence: 99%

Experimental Anaplasmosis in American Bison: Persistence of Infections of Anaplasma Marginale and Non-Susceptibility to A. Ovis

Zaugg

1986

Journal of Wildlife Diseases

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Blood collected 314 and 496 days after experimentally infecting splenectomized and spleen-intact American bison (Bison bison) with Anaplasma marginale was infective for splenectomized bovine steers. The pathogenesis was identical to that seen in bovine studies using bovine blood inoculations. A splenectomized bison remained normal clinically, hematologically and serologically for 10 mo after repeated inoculation of ovine blood infected with A. ovis.

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Comparison of rapid card agglutination test with the complement fixation test for diagnosis of Anaplasma marginale infection in Cattle in Colombia

Cited by 10 publications

References 4 publications

Detection of an Anaplasma marginale common surface protein present in all stages of infection

Detection of an Anaplasma marginale common surface protein present in all stages of infection

Seroprevalence Survey for Anaplasma marginale‐Infection of Austrian Cattle

Experimental Anaplasmosis in American Bison: Persistence of Infections of Anaplasma Marginale and Non-Susceptibility to A. Ovis

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