Comparison of redox state of cells of tatar buckwheat morphogenic calluses and non-morphogenic calluses obtained from them

Kamalova, G. V.; Akulov, A. N.; Rumyantseva, N. I.

doi:10.1134/s0006297909060145

Cited by 8 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most important reasons for the nucleus damage appears to be connected with the high level of oxidative stress, which is a normal physiological state for the NC and its cells appear to be adapted to it. As was demonstrated earlier [21], the NC cells of F . tataricum are in a state of continuous oxidative stress, which is reflected by their higher content of hydrogen peroxide and malonic dialdehyde, low catalase activity and high activity of superoxide dismutase compared to the MC cells.…”

Section: Resultssupporting

confidence: 77%

“…The NCs, which are formed exclusively from parenchymous type cells (Fig 4A and 4B), differed from the MCs because of their friable structure, high growth rate and complete loss of the capacity for morphogenesis [21]. In the NC cells, the central vacuole occupies most of the cell and the nucleus has a near-wall position (Fig 4B).…”

Section: Resultsmentioning

confidence: 99%

“…Such changes can lead to the regeneration of polyploids, chimeric plants or even to the loss of morphogenic potential [2, 47, 48]. The calli of Tartary buckwheat that were obtained from immature embryos differed significantly from the non-morphogenic clones in terms of their morphogenetic potential, the types and structure of the cells and biochemical properties [21]. Our flow cytometry analyses revealed significant differences in the relative DNA content patterns between the distinct callus lines of F .…”

Section: Resultsmentioning

confidence: 99%

“…), which has been reported to maintain its ability to undergo somatic embryogenesis and its low chromosome variability through several years of culture [20, 21]. Tartary buckwheat (2n = 16) is a wild buckwheat species that is considered to be a weed in Europe.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

et al. 2017

View full text Add to dashboard Cite

Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the non-morphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2–3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines.

show abstract

Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Interestingly, for both JIM8 and JIM13, we found a visible increase of the signal intensity in the NC. As was demonstrated in some earlier studies, the NC is under constant oxidative stress (Kamalova et al 2009) and AGPs are known to be involved in the salt stress response (Lamport et al 2006;Olmos et al 2017). Thus, it is likely that during such stress conditions, they may act as a plasticiser to loosen the pectin network or as a cell wall stabiliser.…”

Section: Discussionmentioning

confidence: 74%

Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

Betekhtin

Piński

Milewska‐Hendel

et al. 2019

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The morphogenic callus (MC) of Fagopyrum tataricum contains a large amount of flavonoids, especially rutin, and exhibits a high level of antioxidant activity. A non-morphogenic callus (NC) may appear on the surface of the MC after two to three years of cultivation and is then subjected to a consistently high level of oxidative stress. The elucidation of the molecular background of this instability is essential for gaining a better understanding of the somaclonal variation mechanisms in tissue cultures that have different morphogenic potentials. Thus, in this study we show that continuous oxidative stress in a NC might be connected with a rapid senescence process and as a result, in the upregulation of the genes that are connected with the telomere complexity, ethylene biosynthesis and the expression of DNA methyltransferases. Moreover, we analysed the presence of the hydroxyproline-rich glycoproteins in the calli and demonstrated the differences between the MC and NC. The LM2 antibody can be useful as a marker of the cells in the MC that are embryogenically determined, while the MAC207 antibody seems to be a positive marker of a MC as its signal was absent in the NC. This study also provides the first report on the effect of trichostatin A on the DNA methyltransferases and demethylases in a MC. Key message LM2 can be used as a marker of the embryogenically determined cells. Overproduction of ethylene is present in the NC.

show abstract

Global epigenetic analysis revealed dynamic fluctuations in levels of DNA methylation and histone modifications in the calli of Fagopyrum with different capacity for morphogenesis

Tomasiak,

Sala-Cholewa,

Berg

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Buckwheat characterises with high susceptibility to in vitro tissue culture conditions, which have been researched extensively to study a plethora of processes. F. tataricum morphogenic callus (MC) is characterised by its capacity for morphogenesis for up to ten years of culture, displaying an extraordinary level of genome stability, and comprises of proembryogenic cell complexes (PECC),which are the structures resembling somatic embryos arrested on the pre-globular stage. The non-morphogenic callus (NC) that appears on the surface of MC after approximately two years of culture due to endoreduplication cycles, is characterised by aneuploidy, rapid growth rate and high level of oxidative stress. F. esculentum embryogenic callus (EC) has different morphological and histological features, remains stable for up to three years of culture, has a dense, globular structure, and is capable of forming embryoids from the masses of embryogenic cells, but does not produce a non-embryogenic clone. In this work, immunocytochemical analyses revealed dynamic epigenetic changes in Fagopyrum calli. We demonstrated that; decreased level of H3K4me2 seems to be associated with pluripotency acquisition in F. esculentum EC and F. tataricum MC; DNA hypomethylation appears to be connected with the acquisition of the embryogenic potential and PECC reinitiation in F. tataricum MC. Moreover, we observed that H4K16ac and H4K5ac exhibited the highest variability during the course of passage in NC. Elevated levels of these modifications on day zero and day six for H4K16ac and H4K5ac, respectively, seem to be connected with endoreplication peaks, the processes which are characteristic of this callus.

show abstract

Comparison of redox state of cells of tatar buckwheat morphogenic calluses and non-morphogenic calluses obtained from them

Cited by 8 publications

References 42 publications

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

Global epigenetic analysis revealed dynamic fluctuations in levels of DNA methylation and histone modifications in the calli of Fagopyrum with different capacity for morphogenesis

Contact Info

Product

Resources

About