Comparison of salivary hemoglobin measurements for periodontitis screening

Abstract

Salivary hemoglobin (Hb) for screening of periodontitis is approved under the pharmaceutical affairs law of Japan. Two reagents are commercially available for the modified fecal occult blood test: Saliva Hemo Plus and OC-AUTO S Latex Reagent. We simultaneously measured split specimens from 561 samples by using these two methods and compared the differences and agreement between both methods. Moreover, saliva samples were collected from 10 subjects at five time points during the day for analysis of circadian variations and fluctuation. The Pearson's correlation coefficient for these two reagents was 0.794. The Bland-Altman plot of differences in salivary Hb levels measured by the two reagents indicated that the difference included fixed errors (0.55 μg/mL). On analysis of circadian variations, no statistically significant differences were observed using the Friedman test. However, fixed errors were observed between wake-up time and before dinner and before lunch and before dinner, and no random errors were observed by Bland-Altman analysis. In conclusion, the salivary Hb levels measured using OC-AUTO S Latex Reagent were lower than those measured using Saliva Hemo Plus, along with a tendency for higher levels in the morning. Thus, when performing salivary tests these observations must be considered.

Effect of tablets containing lactoferrin and lactoperoxidase on gingival health in adults: A randomized, double‐blind, placebo‐controlled clinical trial

“…Thirteen subjects were excluded from the analysis of efficacy because they did not attend the 12‐week visit for personal reasons, resulting in lack of data. The baseline salivary hemoglobin test indicated gingival bleeding in some subjects, and the principal investigator judged that 25 subjects with salivary hemoglobin levels exceeding the reported cutoff value (2.0 μg/mL) were inappropriate for accurate analysis of the effects of the intervention in healthy persons. The remaining 109 healthy subjects were included in the efficacy analysis as the per‐protocol set.…”

“…The saliva was immediately transferred into a diluent solution with preserving agents and kept at 4°C. Salivary hemoglobin levels were measured using an OC Hemodia Auto III (Eiken Chemical), and the cutoff point for periodontitis was set at 2.0 μg/mL 16 …”

Oral health of older residents in care and community dwellers: nursing implications

“…In particular, the salivary haemoglobin test requires little training for examiners and minimal calibration (Okada et al. ), and it costs 10 USD per sample (Nomura et al. ).…”

“…), and it was approved as a diagnostic method for periodontal screening in Japan (Okada et al. ). However, the model combining salivary haemoglobin levels and other factors, such as age and self‐reported problems, showed stronger predictive power for the prevalence of active periodontal disease than the one using only salivary haemoglobin (Maeng et al.…”

“…; Okada et al. ). Because it has been shown that blood can leak into saliva as a result of periodontal disease, the presence of haemoglobin contamination in saliva is more common in individuals with periodontal disease (Kamodyová et al.…”