Comparison of <scp>ABO</scp> antibody titration, <scp>IgG</scp> subclasses and qualitative haemolysin test to reduce the risk of passive haemolysis associated with platelet transfusion

Bastos, Eduardo Peres; Castilho, Lilian; Bub, Carolina Bonet; Kutner, José Mauro

doi:10.1111/tme.12687

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…A recent study found a good correlation between high anti-A and anti-B IgM titres with haemolysis ( 34 ). The high titre of IgM and IgG antibodies can cause complement-mediated red cell lysis ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

“…The IgM antibodies can bind to C1q and initiate a complement cascade, leading to membrane attack, complex formation and consequent cell lysis. The IgG antibodies, particularly the IgG1 and IgG3 subclasses, can also fix complement efficiently, thus causing mild to fatal HTRs ( 34 ). A recent study by Bastos et al found that the IgG1 and IgG3 types of ABO antibodies correlate well with haemolysis ( P ≤ 0.001) ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

“…The IgG antibodies, particularly the IgG1 and IgG3 subclasses, can also fix complement efficiently, thus causing mild to fatal HTRs ( 34 ). A recent study by Bastos et al found that the IgG1 and IgG3 types of ABO antibodies correlate well with haemolysis ( P ≤ 0.001) ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

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Characteristics of ABO Antibodies in Group O Malaysian Blood Donors Contributors

Saidin,

Mohd Noor,

Mohamed Yusoff

et al. 2023

MJMS

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Background: Haemolytic transfusion reactions (HTRs) due to anti-A and anti-B antibodies in Group O blood products are rare but potentially fatal. This study aimed to identify the prevalence of high ABO antibody titre and the immunoglobulin (Ig) classes (IgM only or with IgG) and the prevalence of haemolysin antibodies in Group O blood donors. Methods: Plasma from Group O blood donors was tested by using antibody titration at room temperature. Titres ≥ 64 were considered high. The plasma was treated with 0.01 M dithiothreitol (DTT) to determine the presence of IgG antibodies and titre. IgG titres ≥ 64 were considered high. Tests for haemolysis were conducted by mixing the plasma with 3% fresh A1 and B cell suspensions and incubating at 37 °C. The haemolysis was observed macroscopically. Results: Of 311 donors, 238 (76.5%) showed high anti-A and/or anti-B antibody titres. The highest antibody titre obtained was 256. Female and younger donors (< 40 years old) had higher anti-A and anti-B titres. The anti-B titre showed an association with gender (P < 0.001), and was high in female donors (77.8%). Males aged over 50 years old were found to have low mean titre antibodies. Most donors had both IgM and IgG ABO antibodies. The prevalence of haemolysins in our population was 3.5%. Conclusion: Most of our O blood donors had a high ABO antibody titre but a low prevalence of haemolysins. Males aged over 50 years old are the best O donors for preventing HTRs, particularly when mismatch transfusion is required. We recommend a transfusion unit screen for ABO antibody titre in younger female donors (< 40 years old), to prevent the transfusion of high titre O blood products into non-O recipients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characteristics of ABO Antibodies in Group O Malaysian Blood Donors Contributors

Saidin,

Mohd Noor,

Mohamed Yusoff

et al. 2023

MJMS

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“…Given that some microorganisms can stimulate antibodies to blood group antigens, these individuals may have had other infections at the time of sampling, or the antibodies could have been induced by consuming probiotics [ 49 ]. In addition, physiological variations in anti-A and anti-B titres between individuals of the same blood type, including individuals with low- and high-titre isoagglutinins, have been described [ 19 , 20 , 49 , 50 ]. Information regarding the presence of infectious disorders other than SARS-CoV-2, probiotic use at the time of sampling and a history of an ABO-incompatible pregnancy were not evaluated either in patients or in controls.…”

Section: Discussionmentioning

confidence: 99%

Lower Levels of ABO Anti-A and Anti-B of IgM, IgG and IgA Isotypes in the Serum but Not the Saliva of COVID-19 Convalescents

Matzhold

Körmöczi

Banfi

et al. 2022

JCM

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Individuals with ABO type O, naturally possessing anti-A and anti-B antibodies in their serum, are underrepresented among patients infected with SARS-CoV-2 compared with healthy controls. The ABO antibodies might play a role in the viral transmission. Therefore, we aimed to quantify anti-A/anti-B, including their subclasses IgM, IgG and IgA, in the serum and saliva of Caucasians (n = 187) after mild COVID-19 to compare them with individuals who had never been infected with SARS-CoV-2. Two samples were collected within two months after the diagnosis (median days: 44) and two months later. ABO antibodies were determined by flow cytometry. Additionally, total IgA in saliva and antibodies specific to SARS-CoV-2 were tested by ELISA. COVID-19 convalescents had significantly lower levels of anti-A/anti-B IgM, IgG and IgA in their serum than control subjects (p < 0.001). Interestingly, no significant differences were observed in saliva. ABO antibody levels remained stable over the period considered. No relation of ABO to the level of SARS-CoV-2-specific antibodies was observed. Total IgA was lower in convalescents than in controls (p = 0.038). Whereas ABO antibodies in the saliva may not contribute to the pathogenesis of COVID-19, individual pre-existing high serum concentrations of anti-A/anti-B may have a protective effect against SARS-CoV-2 infection.

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“…We used 5% RBCs suspension for A 1 , B and O cells while some studies used 0.8% and 3% RBCs suspension for testing. Other variations include classifying the titer of hemolysins on the basis of degree of hemolysins (Bastos et al, 2020). Moreover, the gold standard method for titer determination is flow cytometry, especially in the case of ABO-incompatible transplant recipients (Warner and Nester, 2006).…”

Section: Discussionmentioning

confidence: 99%

Anti-A and Anti-B Titer Among Blood Group O Donors at a Tertiary Care Centre Lahore, Pakistan

Syyam¹,

Saqlain²,

Hareem³

et al. 2022

PJZ

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Comparison of ABO antibody titration, IgG subclasses and qualitative haemolysin test to reduce the risk of passive haemolysis associated with platelet transfusion

Cited by 7 publications

References 26 publications

Characteristics of ABO Antibodies in Group O Malaysian Blood Donors Contributors

Characteristics of ABO Antibodies in Group O Malaysian Blood Donors Contributors

Lower Levels of ABO Anti-A and Anti-B of IgM, IgG and IgA Isotypes in the Serum but Not the Saliva of COVID-19 Convalescents

Anti-A and Anti-B Titer Among Blood Group O Donors at a Tertiary Care Centre Lahore, Pakistan

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