Comparison of specific endophytic bacterial communities in different developmental stages of Passiflora incarnata using culture‐dependent and culture‐independent analysis

Abstract

Plants and endophytic microorganisms have coevolved unique relationships over many generations. Plants show a specific physiological status in each developmental stage, which may determine the occurrence and dominance of specific endophytic populations with a predetermined ecological role. This study aimed to compare and determine the structure and composition of cultivable and uncultivable bacterial endophytic communities in vegetative and reproductive stages (RS) of Passiflora incarnata. To that end, the endophytic communities were assessed by plating and Illumina‐based 16S rRNA gene amplicon sequencing. Two hundred and four cultivable bacterial strains were successfully isolated. From the plant’s RS, the isolated strains were identified mainly as belonging to the genera Sphingomonas, Curtobacterium, and Methylobacterium, whereas Bacillus was the dominant genus isolated from the vegetative stage (VS). From a total of 133,399 sequences obtained from Illumina‐based sequencing, a subset of 25,092 was classified in operational taxonomy units (OTUs). Four hundred and sixteen OTUs were obtained from the VS and 66 from the RS. In the VS, the most abundant families were Pseudoalteromonadaceae and Alicyclobacillaceae, while in the RS, Enterobacteriaceae and Bacillaceae were the most abundant families. The exclusive abundance of specific bacterial populations for each developmental stage suggests that plants may modulate bacterial endophytic community structure in response to different physiological statuses occurring at the different plant developmental stages.

Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

“…However, measuring the spatiotemporal dynamics of PGPR in the environment remains challenging [18,19]. Various culture-dependent and culture-independent methods have been employed to track and quantify bio-inoculants in the soil [20,21]. However, culture-dependent methods are only successful under sterile conditions, and less than 1% of the soil microbial diversity is recovered with such methods [21,22].…”

“…Various culture-dependent and culture-independent methods have been employed to track and quantify bio-inoculants in the soil [20,21]. However, culture-dependent methods are only successful under sterile conditions, and less than 1% of the soil microbial diversity is recovered with such methods [21,22]. On the other hand, culture-independent methods, such as reporter nucleic acid-based, gene-based, and immunological methods, are capable of detecting less abundant, slow-growing, and unculturable bacteria [8][9][10].…”

Endophytic Bacterial Microbiome Diversity in Early Developmental Stage Plant Tissues of Wheat Varieties

“…and Corynebacterium sp. were found sporadically in the endophytic community of the in vitro cultured wheat varieties, but all of them were reported to be a part of endomicrobiome [45][46][47][48].…”

Multiple Plant Growth-Promotion Traits in Endophytic Bacteria Retrieved in the Vegetative Stage From Passionflower

“…Fifty-eight endophytic bacteria, provided by the Microbial Resources Division of the Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas, were characterized in this study. These bacteria were isolated from leaf tissues in the vegetative stage of P. incarnata by Goulart et al (2019) . The passionflower leaves were collected in January 2015 from the Centroflora Group agricultural fields located at Botucatu, São Paulo, Brazil.…”

“…They are mainly located in the extracellular fluids and, in some cases, inside the cells ( Compant et al, 2010 ), where they may interact with each other and with the host to assemble a specific community in distinct compartments of the plant. The structure and composition of endophytic communities are determined by environment and plant-associated factors, such as the plant genotype, the developmental stage, phenology, and edaphic properties ( Seghers et al, 2004 ; Van Overbeek and Van Elsas et al, 2008 ; de Silva et al, 2016 ; Goulart et al, 2019 ).…”